Composition for use as an assay reagent

Patent metadata

Field	Value
Publication number	US-2016376464-A1
Application number	US-201615259768-A
Country	US
Kind code	A1
Filing date	Sep 8, 2016
Priority date	Jun 14, 2010
Publication date	Dec 29, 2016
Grant date	—

Abstract

A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.

First claim

1 .- 5 . (canceled)

6 . A copolymer of the formula:

wherein:
A is O or NR 1 wherein R 1 is H or alkyl of from 1 to 6 carbon atoms;
n is 1 to 10;
G′ is CHO; CH(OR 8 ) 2 wherein R 8 is alkyl of from 1 to 6 carbon atoms; a member of a specific binding pair;

D′ is
(i)

wherein: w is 2-4; or
(ii) —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′

wherein:
X is O or NR 2 wherein R 2 is H or alkyl of from 1 to 6 carbon atoms;
Y is a polyether group of the formula —(CH 2 O) m — wherein m is 2 to 1500, or >N ⊕ (R 3 R 4 ) wherein R 3 and R 4 are independently H or alkyl of from 1 to 6 carbon atoms;
p is 0 to 10, being at least 1 when Y is >N ⊕ (R 3 R 4 );
q is 0 or 1;
r is 0 to 10, being at least 1 when Y is >N ⊕ (R 3 R 4 ); and
Z′ is SO 3 − ; or alkyl of from 1 to 6 carbon atoms;
R 5 , R 6 and R 7 are independently H or alkyl of from 1 to 6 carbon atoms; and
x and y are independently 1 to about 1000.

7 . The copolymer according to claim 6 wherein:
A is NR 1 wherein R 1 is H or alkyl of from 1 to 6 carbon atoms;
n is 1 to 10;
G′ is CHO; CH(OR 8 ) 2 wherein R 8 is alkyl of from 1 to 6 carbon atoms; or a member of a specific binding pair;
D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
X is O;
Y is >N ⊕ (R 3 R 4 ) wherein R 3 and R 4 are independently H or alkyl of from 1 to 6 carbon atoms;
p is 1 to 10;
q is 1;
r is 1 to 10;
Z′ is SO 3 − ;

R 5 , R 6 and R 7 are independently H or alkyl of from 1 to 6 carbon atoms; and
x and y are independently 1 to 1000.

8 . The copolymer according to claim 7 wherein:
A is NH;
n is 1;
G′ is CHO; CH(OR 8 ) 2 wherein R 8 is methyl; or a member of a specific binding pair;
D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
X is O;
Y is >N ⊕ (R 3 R 4 ) wherein R 3 and R 4 are both methyl;
p is 2;
q is 1;
r is 3;
Z′ is SO 3 − ;

R 5 and R 6 are independently H or methyl and R 7 is H; and
x and y are independently 1 to 1000.

9 . The copolymer according to claim 6 wherein:
A is NR 1 wherein R 1 is H or alkyl of from 1 to 6 carbon atoms;
n is 1 to 10;
G′ is CHO; CH(OR 8 ) 2 wherein R 8 is alkyl of from 1 to 6 carbon atoms; or a member of a specific binding pair;
D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
X is O;
Y is a polyether group of the formula —(CH 2 O) m — wherein m is 2 to 1500;
p is 0;
q is 1;
r is 0;

Z′ is methyl;

R 5 , R 6 and R 7 are independently H or alkyl of from 1 to 6 carbon atoms; and
x and y are independently 1 to 1000.

10 . The copolymer according to claim 9 wherein:
A is NH;
n is 1;
G′ is CHO; CH(OR 8 ) 2 wherein R 8 is methyl; or a member of a specific binding pair;
D′ is —C(O)—X—(CH 2 ) p —(Y) q —(CH 2 ) r —Z′ wherein:
X is O;
Y is a polyether group of the formula —(CH 2 O) m — wherein m is 1100 or 300;
p is 0;
q is 1;
r is 0;
Z′ is methyl;

R 5 and R 6 are independently H or methyl and R 7 is H; and
x and y are independently 1 to 1000.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Lele Bhalchandra
• Singh Pratap
• Bartz Raphael
• Kellogg Jason A

Classifications

• G01N33/582

  with fluorescent label · CPC title

• C08F220/26

  Esters containing oxygen in addition to the carboxy oxygen · CPC title

• C09D139/06

  Homopolymers or copolymers of N-vinyl-pyrrolidones · CPC title

• C08F126/10

  N-Vinyl-pyrrolidone · CPC title

• C08F220/06

  Acrylic acid; Methacrylic acid; Metal salts or ammonium salts thereof · CPC title