Glycosaminoglycans

Patent metadata

Field	Value
Publication number	US-2016375099-A1
Application number	US-201615262895-A
Country	US
Kind code	A1
Filing date	Sep 12, 2016
Priority date	Sep 11, 2008
Publication date	Dec 29, 2016
Grant date	—

Abstract

Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

First claim

1 . A method of treatment of injury or disease, the method comprising administering, to an individual in need thereof, a therapeutically effective amount of an isolated or substantially purified HS/BMP2, wherein HS/BMP2 is heparan sulphate which is capable of specific binding to SEQ ID NO:1 or 6, and to BMP2, and which is obtainable by a method comprising:
(i) providing a solid support having polypeptide molecules adhered to the support, wherein the polypeptide consists of the amino acid sequence QAKHKQRKRLKSSCKRHP [SEQ ID NO:1] or QAKHKQRKRLKSSCKRH [SEQ ID NO:6]; (ii) contacting the polypeptide molecules with a heparan sulphate fraction obtained from mammalian tissue or extracellular matrix such that polypeptide-heparan sulphate complexes are allowed to form; (iii) partitioning polypeptide-heparan sulphate complexes from the remainder of the mixture; (iv) dissociating the heparan sulphate from the polypeptide-heparan sulphate complexes by disrupting the polypeptide-heparan sulphate complexes; and (v) collecting the dissociated heparan sulphate; wherein the method comprises the repair and/or regeneration of bone.

2 . The method of claim 1 wherein the method is for the treatment of bone defect or bone fracture.

3 . The method of claim 2 wherein the method is a treatment of a fracture of a long bone, short bone, flat bone, irregular bone or sesamoid bone.

4 . The method of claim 2 wherein the method is a treatment of a fracture or defect of a skeletal bone, bone of the cranio-facial region, bone of the axial skeleton, appendicular bone or bone of the pelvic skeleton.

5 . The method of claim 4 wherein the method is a treatment of a fracture or defect of a bone of the head, neck, face, jaw, nose or cheek.

6 . The method of claim 1 wherein the method is for the repair or regeneration of bone during dental or facial or cranial surgery, or a spinal disc fusion treatment.

7 . The method of claim 2 wherein the bone defect or bone fracture is a critical or non-union bone defect regeneration, compression fracture, compacted fracture, spiral fracture, complete and incomplete fractures, transverse, linear and oblique fractures or comminuted fractures.

8 . The method of claim 1 wherein the method is for the treatment of osteoporosis or osteoarthritis.

9 . The method of claim 1 , wherein the method involves administering the HS/BMP2 directly to the site of injury or disease in order to stimulate the growth, proliferation and/or differentiation of new tissue to effect a repair of the injury or to cure or alleviate the disease condition.

10 . The method of claim 2 , wherein the method involves injecting HS/BMP2 to the tissue surrounding the fracture.

11 . The method of claim 1 wherein the method involves surgically implanting a biocompatible implant or prosthesis comprising HS/BMP2.

12 . The method of claim 1 , wherein the method further comprises administering mesenchymal stem cells to the patient.

13 . The method of claim 1 , wherein the method further comprises administering BMP2 to the patient.

14 . The composition according to claim 1 which binds to SEQ ID NO:1 with a K D of less than 1 μM.

Assignees

• Agency Science Tech & Res

Inventors

• Cool Simon Mckenzie
• Nurcombe Victor
• Dombrowski Christian

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P19/00

  Drugs for skeletal disorders · CPC title

• A61P17/02

  for treating wounds, ulcers, burns, scars, keloids, or the like · CPC title

• A61P19/08

  for bone diseases, e.g. rachitism, Paget's disease · CPC title

• A61L2300/252

  Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines · CPC title