Magnetic particle separating device, and method of separating and purifying nucleic acid or protein using same

Patent metadata

Field	Value
Publication number	US-2016368001-A1
Application number	US-201415103102-A
Country	US
Kind code	A1
Filing date	Dec 5, 2014
Priority date	Dec 9, 2013
Publication date	Dec 22, 2016
Grant date	—

Abstract

The present invention relates to a magnetic particle separating device, and a method of separating and purifying nucleic acid or protein using the same. The device comprises: induction magnets ( 100 ); an induction magnet fixing part ( 200 ) having induction magnet fixing holes ( 210 ) for fixing the induction magnets ( 100 ); and a body ( 300 ) in which entry holes ( 310 ) are formed into which tubes (T) are inserted. Thus, the application and removal of a magnetic field to and from the body is made very convenient, so that the device can be very advantageously used in the separation and purification of nucleic acid or protein.

First claim

1 . A magnet particle separating device comprising:
a plurality of induction magnets ( 100 ) with magnetic fields for attracting magnetic particles; an induction magnet fixing part ( 200 ) having induction magnet fixing holes ( 210 ) into which the induction magnets ( 100 ) are insulted for fixing the induction magnets ( 100 ); and a body ( 300 ) with which the induction magnet fixing part ( 200 ) is combined, the body ( 300 ) having entry holes ( 310 ) along a height direction such that the entry holes ( 310 ) correspond to array position of the induction magnets ( 100 ), and the body ( 300 ) having a compartment wall ( 320 ) for comparting one ( 310 a ) of the entry holes ( 310 ) and an adjacent entry hole ( 310 b ).

2 . The magnet particle separating device of claim 1 , wherein the compartment wall ( 320 ) comprises:
an insertion hole ( 321 ) formed along a longitudinal direction of the compartment wall ( 320 ), and a tube-fixing member ( 322 ) inserted into the insertion hole ( 321 ) and compressing outer surface of a tube (T) to be inserted into the entry hole ( 310 ) to fix the tube T to the body ( 300 ).

3 . The magnet particle separating device of claim 1 , wherein the body ( 300 ) comprises:
a groove portion ( 360 ) penetrating the compartment wall ( 320 ) along a longitudinal direction of the compartment wall ( 320 ) and formed at inside wall ( 312 ) for forming the entry hole ( 310 ); and a tube-fixing member ( 322 ) inserted into the groove portion ( 360 ) and compressing outer surface of a tube (T) to be inserted into the entry hole ( 310 ) to fix the tube T to the body ( 300 ).

4 . The magnet particle separating device of claim 1 , wherein a depth (D) of the entry hole ( 310 ) is greater than a length (L) of the tube (T) such that a bottom surface (BS) of the tube (T) is spaced apart from a bottom surface ( 311 ) of the entry hole ( 310 ) by a distance (t) when the tube (T) is inserted into the entry hole ( 310 ).

5 . The magnet particle separating device of claim 1 , wherein an end portion ( 211 ) of the induction magnet fixing holes ( 210 ) protrudes for fixing the induction magnet ( 100 ) when the induction magnet is inserted into the induction magnet fixing holes ( 210 ).

6 . The magnet particle separating device of claim 1 , wherein the induction magnet fixing part ( 200 ) has a first combination magnet fixing hole ( 220 ) formed at edge portion of a surface on which the induction magnet fixing holes ( 210 ) are formed, the first combination magnet fixing hole ( 220 ) receiving a first combination magnet ( 230 ) for a combination with the body ( 300 ), and
wherein the body ( 300 ) has a second combination fixing hole ( 330 ) formed at position corresponding to the first combination magnet fixing hole ( 220 ), the second combination fixing hole ( 330 ) receiving a second combination magnet ( 340 ) for a combination with the induction magnet fixing part ( 200 ) through a magnetic force.

7 . The magnet particle separating device of claim 1 , wherein a first end of the induction magnets ( 100 ) is fixed to the induction magnet fixing holes ( 210 ) of the induction magnet fixing part ( 200 ), and a second end opposite to the first end protrudes upward, and
the entry hole ( 310 ) has an upper portion and a lower portion smaller than the upper portion in a cross-sectional area, for providing a lower portion of the body ( 300 ) with a space for the induction magnets ( 100 ) to be disposed between the compartment wall ( 320 ) and an adjacent compartment wall ( 320 a ).

8 . The magnet particle separating device of claim 7 , further comprising a body position fixing part ( 400 ) disposed between the body ( 300 ) and the induction magnet fixing part ( 200 ), the body position fixing part ( 400 ) comprising:
a compressing member ( 410 ) for compressing an outer surface of the body ( 300 ) for the body ( 300 ) to be fixed to the induction magnet fixing part ( 200 ) to which the induction magnets ( 100 ) are fixed; and a receiving portion ( 420 ) receiving and fixing the compression member ( 410 ).

9 . A method of separating and purifying nucleic acid or protein using the magnet particle separating device of claim 1 , the method comprising:
mixing magnetic particles with gene material to provide a mixture to the tube (T) in order to separate nucleic acid by using magnetic particles (S 100 ); fixing the tube to the body ( 300 ) (S 200 ); combining the induction magnet fixing part ( 200 ) with the induction magnets ( 100 ) fixed thereto with the body ( 300 ) (S 300 ); attracting magnetic particles combined with the gene material toward the induction magnets ( 100 ) through magnetic fields generated by the induction magnets ( 100 ) (S 400 ); removing residue solution except for the magnetic particles combined with the gene material from the from the tube (T) (S 500 ); and separating the induction magnet fixing part ( 200 ) from the body ( 300 ) to remove magnetic fields applied to the body ( 300 ) (S 600 ).

10 . The method of claim 9 , further comprising:
providing cleaning solution or separation solution to the tube (T) for separate the nucleic acid or protein from the magnetic particles in the tube (T) (S 700 ) after removing magnetic fields (S 600 ).

Assignees

• Bioneer Corp

Inventors

• Park Han Oh
• Kim Jong Kab

Classifications

• B01L9/06

  Test-tube stands; Test-tube holders · CPC title

• G01N1/405

  by adsorption or absorption · CPC title

• B03C1/0332

  using permanent magnets · CPC title

• B03C1/01

  by addition of magnetic adjuvants · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title