Method for separating and concentrating target substance using novel cationic graft polymer

Patent metadata

Field	Value
Publication number	US-2016367979-A1
Application number	US-201515120820-A
Country	US
Kind code	A1
Filing date	Feb 27, 2015
Priority date	Mar 24, 2014
Publication date	Dec 22, 2016
Grant date	—

Abstract

The purpose of the present invention is to provide a method for separating and concentrating a target substance as an alternative to existing cationic polymers, and a kit for implementing this method.

First claim

1 . A method for separating and concentrating a target substance using a cationic graft polymer, comprising:
(1) step of bringing the cationic graft polymer into contact with a sample containing the target substance to make the target substance bound to the cationic graft polymer under a basic condition; (2) step of separating the bound complex of the cationic graft polymer and the target biomolecule; and (3) step of eluting the target substance from the bound complex, wherein the cationic graft polymer is a polyamine graft polymer obtained by polymerizing a polyamine derivative obtained by a reaction between (a) a polymer compound having at least one amino group and (b) a compound having at least one epoxy group, and (c) an ethylenically unsaturated monomer.

2 . The method according to claim 1 , further comprising adding a divalent or trivalent metal ion in step (1).

3 . The method according to claim 1 , further comprising a step for washing the bound complex in step (2).

4 . The method according to claim 1 , wherein the cationic graft polymer is fixed to a solid phase surface.

5 . The method according to claim 1 , comprising a step for aggregating a cationic graft polymer which is not bound to the target substance in step (3).

6 . A kit used for the method according to claim 1 , comprising a cationic graft polymer.

7 . The kit according to claim 6 , further comprising a divalent or trivalent metal ion salt.

8 . The kit according to claim 6 , further comprising a reaction solution containing a basic solution and a divalent or trivalent metal ion salt.

9 . The kit according to claim 6 , further comprising a washing solution.

10 . The kit according to claim 6 , further comprising a dispersion solution containing an acidic solution or a chelating agent.

11 . The kit according to claim 6 , further comprising an aggregation solution.

Assignees

• Nitto Boseki Co Ltd

Inventors

• ASHIZAWA Kazuho
• Noda Kenta
• Watanabe Koji
• Teruuchi Yoko
• Bunya Masaru

Classifications

• C12N7/00

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• B01D15/3804

  Affinity chromatography · CPC title

• C12M47/12

  Purification (C12M47/04 takes precedence) · CPC title

• C12N1/02 Primary

  Separating microorganisms from their culture media · CPC title

• B01J20/261

  obtained by reactions only involving carbon to carbon unsaturated bonds (macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F) · CPC title