Methods for preparing a skin graft without culturing or use of biologics
US-9517082-B2 · Dec 13, 2016 · US
US2016367358A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016367358-A1 |
| Application number | US-201514746631-A |
| Country | US |
| Kind code | A1 |
| Filing date | Jun 22, 2015 |
| Priority date | Jun 22, 2015 |
| Publication date | Dec 22, 2016 |
| Grant date | — |
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A method of reproducing a biological object includes 3D-imaging the object and generating a mold using a 3D printer; obtaining cells from a donor site on the object; preparing a live cell suspension using the cells from the donor site; forming a scaffold in the mold with collagen and seeding the scaffold with live cells, growing the cells in the mold and curing the collagen; and surgically implanting the object into a living subject, where the cells continue to live in the living subject.
Opening claim text (preview).
What is claimed is: 1 . A method of reproducing a biological object, comprising: 3D-imaging the object and generating a mold using a 3D printer; obtaining cells from a donor site on the object; preparing a live cell suspension using the cells from the donor site; forming a scaffold in the mold with collagen and seeding the scaffold with live cells, growing the cells in the mold and curing the collagen; and surgically implanting the object into a living subject, where the cells continue to live in the living subject. 2 . The method of claim 1 , comprising auto-grafting a three-dimensional irregularly-shaped object. 3 . The method of claim 1 , comprising printing a quantity of harvested skin cells. 4 . The method of claim 1 , comprising forming a photocrosslinkable interpenetrating polymeric network (IPN) of collagen and hyaluronic acid (HA). 5 . The method of claim 1 , comprising printing a bone fragment. 6 . The method of claim 1 , comprising printing materials with nerve tissues. 7 . The method of claim 1 , comprising printing materials with brain cells, lung cells, pancreatic cells or stem cells. 8 . The method of claim 1 , comprising injecting bio-materials using a syringe in the pen or spraying a mist of the bio-materials using a MEMS actuator. 9 . The method of claim 1 , comprising printing a skin, a nose or ear portion. 10 . The method of claim 1 , comprising transmitting the target area image to a remote medical professional for modification prior to printing. 11 . A method of healing a skin wound includes imaging the skin wound using an imager including a supply cartridge; obtaining dermal cells from a donor site; preparing a live cell suspension using the dermal cells from the donor site and insert the supply cartridge with the live cell suspension into the pen; 3D printing onto the wound region an acellular dermal matrix (ADM) scaffold with collagen and seeded with live cells processed from the autologous graft; and securing with sutures and covering with a suitable bandage. 12 . The method of claim 11 , comprising auto-grafting a three-dimensional irregularly-shaped object. 13 . The method of claim 11 , comprising printing a quantity of harvested skin cells. 14 . The method of claim 11 , comprising forming a photocrosslinkable interpenetrating polymeric network (IPN) of collagen and hyaluronic acid (HA). 15 . The method of claim 11 , comprising surgically implanting the object into a living subject, where the cells continue to live in the living subject. 16 . The method of claim 11 , comprising printing materials with nerve tissues. 17 . The method of claim 11 , comprising printing materials with stem cells. 18 . The method of claim 11 , comprising injecting bio-materials using a syringe in the pen or spraying a mist of the bio-materials using a MEMS actuator. 19 . The method of claim 1 , comprising printing a nose or ear portion. 20 . The method of claim 11 , comprising transmitting the target area image to a remote medical professional.
using three dimensional printing [3DP] · CPC title
Designing or making customized prostheses · CPC title
with macromolecular fillers · CPC title
Collagen · CPC title
involving reference images or patches · CPC title
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