Methods of Storing Information Using Nucleic Acids

Patent metadata

Field	Value
Publication number	US-2016358055-A1
Application number	US-201615175430-A
Country	US
Kind code	A1
Filing date	Jun 7, 2016
Priority date	Jul 19, 2012
Publication date	Dec 8, 2016
Grant date	—

Abstract

The present invention relates to methods of storing data using one or more nucleic acids.

First claim

1 .- 16 . (canceled)

17 . A method of storing text or an image using nucleotides comprising
converting the text or the image into a plurality of bit sequences of a bit stream, converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding, wherein the base is a member of a base pair selected from the group consisting of

synthesizing the plurality of corresponding oligonucleotide sequences using a template-independent polymerase, and
storing the synthesized plurality of corresponding oligonucleotide sequences.

18 . The method of claim 17 wherein the oligonucleotide sequences include one or more or all of a data block sequence, an address sequence specifying the location of the data block in the bit stream, or flanking common sequences at each end of the oligonucleotide for amplification and sequencing.

19 . The method of claim 17 wherein the template-independent polymerase is terminal deoxynucleotidyl transferase or poly(A) polymerase.

20 . The method of claim 17 wherein the oligonucleotide sequences are stored on a solid or semi-solid support.

21 . The method of claim 17 wherein the oligonucleotide sequences are stored on a support selected from the group consisting of a slide, bead, chip, particle, strand, gel, sheet, tubing, sphere, container, capillary, pad, slice, film, and plate.

22 . The method of claim 17 wherein the A or C encodes for a first bit and G or T encodes for a second bit.

23 . The method of claim 17 wherein the A or C encodes for zero and G or T encodes for 1.

Assignees

• Harvard College

Inventors

• Church George M

Classifications

• G06K19/00

  Record carriers for use with machines and with at least a part designed to carry digital markings · CPC title

• G06N3/123

  DNA computing · CPC title

• G06K19/022 Primary

  Processes or apparatus therefor · CPC title

• G16B30/00 Primary

  ICT specially adapted for sequence analysis involving nucleotides or amino acids · CPC title

• G06F19/22

  Physics · mapped topic