Methods of grading carcinomas

Patent metadata

Field	Value
Publication number	US-2016356779-A1
Application number	US-201515117472-A
Country	US
Kind code	A1
Filing date	Feb 9, 2015
Priority date	Feb 10, 2014
Publication date	Dec 8, 2016
Grant date	—

Abstract

Methods for grading carcinomas are provided as well as kits therefor. A method is provided for grading a carcinoma, or suspected carcinoma, comprising obtaining a sample of the carcinoma or suspected carcinoma and contacting the sample with one or more reagents so as to identify LacNAc or Gal.beta.1,4GlcNAc expression on cell surfaces thereof, quantifying the identified LacNAc or Gal.beta.1,4GlcNAc expression, and comparing quantified LacNAc or Gal.beta.1,4GlcNAc expression to one or more predetermined control values, and assigning a grade to the suspected carcinoma based on the quantified LacNAc or Gal.beta.1,4GlcNAc expression being in excess of, or less than, the one or more predetermined control values. Also provided is a method for identifying a sample from a subject as a carcinoma sample. Also provided is a kit for grading carcinoma samples comprising a LacNAc-specific or Gal-1,4GlcNAc-specific glycosylation enzyme and a synthetically-labeled sugar molecule and written instructions for use thereof.

First claim

1 . A method for grading a carcinoma, or a suspected carcinoma, comprising obtaining a sample of the carcinoma or suspected carcinoma and contacting the sample with one or more reagents so as to identify LacNAc or Galβ1,4GlcNAc expression on cell surfaces thereof, quantifying the identified LacNAc or Galβ1,4GlcNAc expression, and comparing quantified LacNAc or Galβ1,4GlcNAc expression to one or more predetermined control values, and assigning a grade to the suspected carcinoma based on the quantified LacNAc or Galβ1,4GlcNAc expression being in excess of, or less than, the one or more predetermined control values.

2 - 3 . (canceled)

4 . The method of claim 1 , wherein the carcinoma is an adenocarcinoma.

5 . The method of claim 1 , wherein the carcinoma is a lung, gastric or breast carcinoma.

6 . The method of claim 1 , wherein LacNAc or Galβ1,4GlcNAc expression on a cell surface is identified by a method comprising contacting the cell with a LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme and a synthetically-labeled sugar molecule so as to permit incorporation of the synthetically-labeled sugar molecule into the LacNAc or Galβ1,4GlcNAc expressed on a cell surface, and then detecting the presence of the synthetically-labeled sugar molecule so as to determine LacNAc or Galβ1,4GlcNAc expression on the cell surface.

7 . The method of claim 6 , wherein quantifying the identified LacNAc or Galβ1,4GlcNAc expression comprises quantifying the amount of synthetically-labeled sugar molecules so as to thereby quantify the level of expression of LacNAc or Galβ1,4GlcNAc on the cell surface.

8 . The method of claim 6 , wherein the LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme is an isolated α1,3 fucosyltransferase.

9 . The method of claim 6 , wherein the LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme has the same amino acid sequence as, or is, a recombinant H. pylori α1,3 fucosyltransferase.

10 . The method of claim 6 , wherein the synthetically-labeled sugar molecule comprises a fucose molecule.

11 . The method of claim 6 , wherein the synthetically-labeled sugar molecule comprises a C6-azide-labeled fucose molecule or a C6-alkyne-labeled fucose molecule.

12 . The method of claim 6 , further comprising tagging the synthetically-labeled sugar molecule with a probe molecule by ligand-assisted CuAAC (azide-alkyne cycloaddition).

13 . The method of claim 12 , further comprising contacting with BTTES28, BTTPS33 or BTTAA29.

14 . The method of claim 1 , wherein the sample is a tissue sample.

15 . The method of claim 1 , wherein the sample is obtained from a subject, and wherein the subject is a human subject.

16 - 28 . (canceled)

29 . A method of treating a hematologic disease that is treatable with human cord blood cells in a subject, comprising administering to the subject an amount of human cord blood cells that have been contacted with a recombinant LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme and a fucose, such that the fucose has been incorporated into the LacNAc or Galβ1,4GlcNAc expressed on one or more cell surfaces of the human cord blood cells, effective to treat the hematologic disease.

30 . The method of claim 29 , wherein the human cord blood cells treated with the recombinant LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme and a fucose have a higher level of cell-surface fucosylation than untreated human cord blood cells.

31 . The method of claim 29 , further comprising contacting human cord blood cells with a recombinant LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme and a fucose, such that the fucose is incorporated into the LacNAc or Galβ1,4GlcNAc expressed on one or more cell surfaces of the human cord blood cells, prior to administering the amount of human cord blood cells that have been contacted with a recombinant LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme and a fucose.

32 . A method of treating a cancer in a subject, comprising administering to the subject an amount of human dendritic cells that have been contacted with a recombinant LacNAc-specific or Galβ1,4GlcNAc-specific glycosylation enzyme and a fucose, such that the fucose has been incorporated into the LacNAc or Galβ1,4GlcNAc expressed on one or more cell surfaces of the human dendritic cells, effective to treat the cancer.

33 - 36 . (canceled)

37 . The method of claim 29 , wherein the fucose is synthetically-labeled.

38 - 43 . (canceled)

Assignees

• Albert Einstein College Of Medicene Inc
• Albert Einstein College Medicine Inc

Inventors

• Wu Peng
• Rouhanifard Sara
• Zheng Tianqing

Classifications

• G01N33/57525

  of the liver or pancreas · CPC title

• G01N33/57515

  of the breast · CPC title

• G01N33/5753

  of the stomach or small intestine · CPC title

• G01N33/5752

  of the lungs · CPC title

• G01N33/5759 Primary

  involving compounds localised on the membrane of tumour or cancer cells · CPC title