Heterodimeric antibodies that bind cd3 and tumor antigens

Patent metadata

Field	Value
Publication number	US-2016355608-A1
Application number	US-201615141350-A
Country	US
Kind code	A1
Filing date	Apr 28, 2016
Priority date	Nov 26, 2014
Publication date	Dec 8, 2016
Grant date	—

Abstract

The present invention is directed to novel heterodimeric antibodies.

First claim

1 .- 50 . (canceled)

51 . A heterodimeric antibody comprising:
a) a first monomer comprising:
i) a first Fc domain;
ii) an anti-CD3 scFv comprising a scFv variable light domain, an scFv linker and a scFv variable heavy domain; wherein said scFv is covalently attached to the N-terminus of said Fc domain using a domain linker;
b) a second monomer comprising a heavy chain comprising:
i) a heavy variable domain; and
ii) a heavy chain constant domain comprising a second Fc domain; and
c) a light chain comprising a variable light domain and a variable light constant domain; wherein said anti-CD3 scFv is selected from the group consisting of anti-CD3 H1.32_L1.47 (SEQ ID NO:18), anti-CD3 H1.89_L1.47 (SEQ ID NO:27), anti-CD3 H1.90_L1.47 (SEQ ID NO:36) and anti-CD3 H1.33_L1.47 (SEQ ID NO:45), and said heavy variable domain and said light variable domain bind a TTA.

52 . A heterodimeric antibody according to claim 51 wherein said TTA is selected from the group consisting of CD19, CD20, CD38 and CD123.

53 .- 72 . (canceled)

73 . A nucleic acid composition comprising:
a) a first nucleic acid encoding said first monomer of claim 51 ; b) a second nucleic acid encoding said second monomer of claim 51 ; and c) a third nucleic acid encoding said light chain of claim 51 .

74 . An expression vector composition comprising:
a) a first expression vector comprising a first nucleic acid encoding said first monomer of claim 51 ; b) a second expression vector comprising a second nucleic acid encoding said second monomer of claim 51 ; and c) a third expression vector comprising a third nucleic acid encoding said light chain of claim 51 .

75 . A host cell comprising said expression vector composition of claim 74 .

Assignees

• Xencor Inc

Inventors

• Bernett Matthew J
• Moore Gregory
• Desjarlais John
• Chu Seung
• Rashid Rumana
• Muchhal Umesh
• Lee Sung-Hyung

Classifications

• C07K2317/73

  Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation · CPC title

• C07K2317/565

  Complementarity determining region [CDR] · CPC title

• C07K2317/522

  CH1 domain · CPC title

• C07K16/30

  from tumour cells · CPC title

• C07K2317/31

  multispecific · CPC title