Combined thermal devices for thermal cycling

Patent metadata

Field	Value
Publication number	US-2016354785-A1
Application number	US-201615056475-A
Country	US
Kind code	A1
Filing date	Feb 29, 2016
Priority date	Jun 30, 2006
Publication date	Dec 8, 2016
Grant date	—

Abstract

The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.

First claim

1 . (canceled)

2 . A nucleic acid amplification system comprising: a microfluidic device comprising at least one microfluidic channel, means to flow a fluid sample through the at least one microfluidic channel, a first heating device, a second heating device, a first temperature sensing device, a second temperature sensing device, and a controller, wherein the first and second heating devices have different temperature resolutions.

3 . The system of claim 2 , wherein the fluid flow means causes a pressure differential.

4 . The system of claim 3 , wherein the fluid flow means is a pump.

5 . The system of claim 2 , wherein the first heat source is a contact heat source.

6 . The system of claim 5 , wherein the first heat source is selected from the group comprising: a Peltier device, a resistive heater or a chemical heater.

7 . The system of claim 2 , wherein the second heat source is a non-contact heat source.

8 . The system of claim 7 , wherein the second heat source is a electromagnetic heat source.

9 . The system of claim 8 , wherein the second heat source is selected from the group comprising an infra-red source, a tungsten filament bulb and laser.

10 . The system of claim 2 , wherein the controller is configured to independently adjust the first and second heating device.

11 . The system of claim 10 , wherein the controller is configured to increase heat from the second heating device when decreasing heat from the first heating device.

12 . The system of claim 2 , wherein the first heating device is thermostatically controlled by the controller based on an output of the first temperature sensing device, which temperature sensing device is operable to sense a temperature of the sample.

Assignees

• Canon Us Life Sciences Inc

Inventors

• Dale Gregory A
• Zeng Shulin
• Hasson Kenton C

Classifications

• B01L2200/143

  Quality control, feedback systems · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title

• B01L7/525 Primary

  with physical movement of samples between temperature zones · CPC title

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title