Structured illumination microscope, structured illumination method, and program
US-2016320600-A1 · Nov 3, 2016 · US
US2016349496A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016349496-A1 |
| Application number | US-201615164052-A |
| Country | US |
| Kind code | A1 |
| Filing date | May 25, 2016 |
| Priority date | May 27, 2015 |
| Publication date | Dec 1, 2016 |
| Grant date | — |
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A control apparatus includes a lens, an SLM presenting a modulation pattern on a modulation plane and outputting modulated light L 2 for forming light spots P 1 and P 2 on a pupil plane of the lens, an imaging device imaging a fringe pattern image formed on a focal plane of the lens and generating image data Da indicating the fringe pattern image, a calculation unit calculating at least one kind of parameter among an intensity amplitude, a phase shift amount, and an intensity average from the image data Da, an analysis unit obtaining a deviation in relative positions of an optical axis of the lens and a reference coordinate of the modulation plane based on the parameter, and a changing unit changing an origin position of the reference coordinate so that the deviation in the relative positions is decreased.
Opening claim text (preview).
What is claimed is: 1 . A control apparatus for a spatial light modulator, comprising: a lens; a spatial light modulator including a modulation plane, in which a plurality of modulation pixels are arrayed two-dimensionally, and configured to modulate light based on a first modulation pattern and output first modulated light for forming a first light spot and a second light spot on a pupil plane of the lens; an image sensor including an imaging plane, in which a plurality of photoelectric conversion pixels are arrayed two-dimensionally, and configured to capture an image of a first fringe pattern image formed on a focal plane of the lens by the first modulated light on the imaging plane and generate first image data indicating a light intensity distribution of the first fringe pattern image; and a processor electrically coupled to the image sensor and the spatial light modulator, and the processor configured to: calculate at least one kind of first parameter among an intensity amplitude, a phase shift amount, and an intensity average based on the first image data, obtain a deviation in relative positions of an optical axis of the lens and a reference coordinate of the modulation plane based on the first parameter, and control an origin position of the reference coordinate on the modulation plane so that the deviation in the relative positions is decreased. 2 . The control apparatus according to claim 1 , further comprising a storage configured to store a data table indicating a known correlation between the deviation in the relative positions of the optical axis of the lens and the reference coordinate of the modulation plane and the at least one kind of parameter among the intensity amplitude, the phase shift amount, and the intensity average, wherein the processor compares the first parameter with the data table for calculating the deviation in the relative positions. 3 . The control apparatus according to claim 1 , wherein the spatial light modulator modulates light based on a second modulation pattern and further outputs second modulated light for forming a third light spot and a fourth light spot, the image sensor captures an image of a second fringe pattern image formed on the focal plane of the lens by the second modulated light on the imaging plane and further generates second image data indicating a light intensity distribution of the second fringe pattern image, and the processor calculates at least one kind of second parameter among an intensity amplitude, a phase shift amount, and an intensity average based on the second image data, and obtains the deviation in the relative positions based on the first parameter and the second parameter. 4 . The control apparatus according to claim 3 , further comprising a storage configured to store a data table indicating a known correlation between the deviation in the relative positions of the optical axis of the lens and the reference coordinate of the modulation plane and the at least one kind of parameter among the intensity amplitude, the phase shift amount, and the intensity average, wherein the processor compares the first parameter and the second parameter with the data table for calculating the deviation in the relative positions. 5 . The control apparatus according to claim 3 , wherein a position of one of the first light spot and the second light spot is identical to a position of one of the third light spot and the fourth light spot. 6 . A control method for a spatial light modulator, comprising: modulating light, by a spatial light modulator, based on a first modulation pattern; outputting first modulated light for forming a first light spot and a second light spot on a pupil plane of a lens from the spatial light modulator; capturing an image of a first fringe pattern image formed on a focal plane of the lens by the first modulated light on an imaging plane of an image sensor; generating first image data indicating a light intensity distribution of the first fringe pattern image; calculating at least one kind of first parameter among an intensity amplitude, a phase shift amount, and an intensity average from the first image data; obtaining a deviation in relative positions of an optical axis of the lens and a reference coordinate of the modulation plane based on the first parameter; and controlling an origin position of the reference coordinate on the modulation plane so that the deviation in relative positions is decreased. 7 . The control method according to claim 6 , wherein in the obtaining, a data table indicating a known correlation between the deviation in the relative positions of the optical axis of the lens and the reference coordinate of the modulation plane and the at least one kind of parameter among the intensity amplitude, the phase shift amount, and the intensity average is compared with the first parameter for calculating the deviation in the relative positions. 8 . The control method according to claim 6 , further comprising: modulating light, by the spatial light modulator, based on a second modulation pattern; outputting second modulated light for forming a third light spot and a fourth light spot on the pupil plane of the lens from the spatial light modulator; capturing an image of a second fringe pattern image formed on the focal plane of the lens by the second modulated light on the imaging plane of the image sensor; generating second image data indicating a light intensity distribution of the second fringe pattern image; and calculating at least one kind of second parameter among an intensity amplitude, a phase shift amount, and an intensity average based on the second image data, wherein in the obtaining, the deviation in the relative positions is obtained based on the first parameter and the second parameter. 9 . The control method according to claim 8 , wherein in the obtaining, a data table indicating a known correlation between the deviation in the relative positions of the optical axis of the lens and the reference coordinate of the modulation plane and the at least one kind of parameter among the intensity amplitude, the phase shift amount, and the intensity average is compared with the first parameter and the second parameter for calculating the deviation in the relative positions. 10 . The control method according to claim 8 , wherein a position of one of the first light spot and the second light spot is identical to a position of one of the third light spot and the fourth light spot.
Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title
Operation of devices; Circuit arrangements, not otherwise provided for in this subclass · CPC title
Means for illuminating specimens · CPC title
for controlling the phase of light (G02B26/08 takes precedence {, measuring optical phase difference G01J9/00}) · CPC title
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