Methods for creating bilayers for use with nanopore sensors
US-12077817-B2 · Sep 3, 2024 · US
US2016349246A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016349246-A1 |
| Application number | US-201415035094-A |
| Country | US |
| Kind code | A1 |
| Filing date | Nov 6, 2014 |
| Priority date | Nov 6, 2013 |
| Publication date | Dec 1, 2016 |
| Grant date | — |
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To provide a method for separating a vesicle having a lipid bilayer membrane, which has excellent efficiency of removing impurities and is unsuch asly to cause disruption of the vesicle having a lipid bilayer membrane. A method for separating the vesicle having the lipid bilayer membrane including: a complex forming step for forming a complex of the vesicle and the solid phase carrier by bringing a biological sample including a vesicle having a lipid bilayer membrane into contact with a solid phase carrier to which a ligand for recognizing a surface antigen present on a surface of the vesicle and is bound, a washing step for washing the complex, wherein at least any of the complex forming step and the washing step is performed in the presence of a nonionic surfactant.
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1 : A method for separating a vesicle having a lipid bilayer membrane, the method comprising: forming a complex of a vesicle and a solid phase carrier by bringing a biological sample comprising the vesicle having the lipid bilayer membrane into contact with the solid phase carrier to which a ligand for recognizing a surface antigen present on a surface of the vesicle and is bound, and washing the complex, wherein the forming and/or the washing is performed in the presence of a nonionic surf…
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