Porous membrane having immobilized enzyme, porous membrane composite including the same, and preparation method thereof

Patent metadata

Field	Value
Publication number	US-2016348090-A1
Application number	US-201615158590-A
Country	US
Kind code	A1
Filing date	May 19, 2016
Priority date	Apr 10, 2013
Publication date	Dec 1, 2016
Grant date	—

Abstract

Disclosed herein is a porous membrane having an immobilized enzyme wherein the enzyme is immobilized within pores which are three-dimensionally connected to each other. The porous membrane having the immobilized enzyme is three-dimensionally crosslinked in a molecular level wherein nanopores of 5 to 100 nm are interconnected, so that the immobilized enzyme may be in contact with a reactant in all directions, and the reaction solution may be easily diffused, thereby proceeding with the catalytic reaction fast and conveniently without deterioration of material transport.

First claim

What is claimed is:

1 . A preparation method of a porous membrane having an immobilized enzyme, comprising (B) passing a solution containing the enzyme through the porous membrane, wherein the porous membrane is three-dimensionally interconnected by pores having a size of 5 to 100 nm; A framework of the porous membrane is a three-dimensional network formed by polymerization of a monomer having 2 to 4 amino groups and a monomer having 2 to 4 isocyanate groups, acyl halide groups or ester groups, mixing with a polymer to form phase-separated structure, and removing the polymer with a solvent.

2 . The method of claim 1 , wherein the passing of the solution is carried out in a manner selected from the group consisting of dead-end flow, cross flow filtration, and a combination thereof.

3 . The method of claim 1 , further comprising (A) obtaining the porous membrane before (B), wherein (A) includes the following:
(a-1) polymerizing a first monomer and a second monomer each having 2 to 4 functional groups to obtain an organic sol; (a-2) adding a polymer solution to the organic sol to obtain a mixed solution; (a-3) applying the mixed solution on a substrate then curing it to obtain a porous membrane, and (a-4) passing solvent through the porous membrane or precipitating the microporous membrane in solvent to remove polymer; the functional group of the first monomer is an amino group, the functional group of the second monomer is an isocyanate group, an acyl halide group or an ester group, and at least one of the first monomer and the second monomer has 4 functional groups.

4 . The method of claim 3 , wherein the polymer solution includes a polymer selected from the group consisting of polyethyleneglycol, polysulfone, polyethersulfone, polyacrylonitrile, polyimide, polyetherimide, polybenzimidazole, polymethylmethacrylate, polystyrene, polyetheretherketone and polyvinylidenefluoride.

Assignees

• Gwangju Inst Science & Tech

Inventors

• Park Ji-Woong
• Han Sun-Young
• Bae Jae-Sung

Classifications

• C12P7/649

  Biodiesel, i.e. fatty acid alkyl esters · CPC title

• C12N11/04 Primary

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• C12N11/08

  the carrier being a synthetic polymer · CPC title

• B01D71/643

  Polyether-imides · CPC title

• C12N11/091

  Phenol resins; Amino resins · CPC title