Mesendoderm and mesoderm cell populations

Patent metadata

Field	Value
Publication number	US-2016348069-A1
Application number	US-201615237079-A
Country	US
Kind code	A1
Filing date	Aug 15, 2016
Priority date	May 17, 2002
Publication date	Dec 1, 2016
Grant date	—

Abstract

The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. In accordance with the present invention, a selectable marker gene has been recombinantly targeted to the brachyury locus to allow the isolation and characterization of cell populations that comprise brachyury positive mesendoderm and mesoderm cells. The cell populations of the invention are useful for generating cells for cell replacement therapy.

First claim

1 .- 28 . (canceled)

29 . A method for generating mammalian cells comprising culturing embryonic stem cells in the absence of serum and in the presence of at least 30 ng/ml of activin for two to ten days to induce differentiation of embryonic stem cells to mesendoderm cells that express brachyury, isolating said mesendoderm cells that express brachyury, culturing said isolated cells to obtain endoderm cells that do not express brachyury, and culturing the endoderm cells under conditions effective for the differentiation of endoderm into liver cells or pancreatic cells.

30 . (canceled)

31 . The method of claim 29 , wherein the embryonic stem cells are cultured in the presence of 100 ng/ml of the activin.

Assignees

• Icahn School Med Mount Sinai

Inventors

• Keller Gordon
• Kouskoff Valerie
• Kubo Atsushi
• Fehling Hans J

Classifications

• A61P3/10

  for hyperglycaemia, e.g. antidiabetics · CPC title

• A61P7/06

  Antianaemics · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P9/04

  Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title