Microscale bioprocessing system and method for protein manufacturing

Patent metadata

Field	Value
Publication number	US-2016348048-A1
Application number	US-201615167743-A
Country	US
Kind code	A1
Filing date	May 27, 2016
Priority date	Mar 8, 2011
Publication date	Dec 1, 2016
Grant date	—

Abstract

A bioprocessing system for protein manufacturing is provided that is compact, integrated and suited for on-demand production and delivery of therapeutic proteins to patients. The system can also be used for efficient on-demand production of any type of protein.

First claim

What is claimed is:

1 . A bioprocessing system, comprising:
a reactor for protein expression; a membrane chromatography component for receiving and purifying protein output by the reactor; and a diafiltration component for receiving purified protein from the membrane chromatography and for further purifying the purified protein.

2 . The system of claim 1 , wherein the reactor is adapted for cell-free protein expression.

3 . The system of claim 2 , wherein the reactor has a capacity of approximately 20 ml or less and the membrane chromatography component has a capacity of less than approximately 5 ml.

4 . The system of claim 1 , wherein the diafiltration component comprises:
a product section for receiving purified protein from the membrane chromatography component; and a buffer section for receiving a buffer solution.

5 . The system of claim 4 , wherein the diafiltration component comprises:
a first substrate; a second substrate; and a diafiltration membrane positioned between the first and second substrates.

6 . The system of claim 5 , wherein the product section comprises a serpentine-shaped channel formed on the first substrate and the buffer section comprises a serpentine-shaped channel formed on the second substrate, wherein the first and second serpentine-shaped channels substantially overlap each other.

7 . The system of claim 4 , wherein the diafiltration component comprises a flow cell.

8 . The system of claim 7 , wherein the product section comprises tubing positioned within the flow cell and the diafiltration membrane comprises the tubing material.

9 . The system of claim 1 , wherein the reactor comprises at least one sensor for monitoring reaction parameters.

10 . The system of claim 9 , wherein the at least one sensor monitors at least one of conductivity, temperature, pH, oxygen and CO 2 .

Assignees

• Univ Maryland

Inventors

• Rao Govind
• Kostov Yordan
• Tolosa Leah
• Lam Hung
• Ge Xudong
• Frey Douglas
• Singh Virendra

Classifications

• C12M21/14 Primary

  for producing enzymes · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C07K1/36

  by a combination of two or more processes of different types · CPC title

• B01D15/3847

  Multimodal interactions · CPC title

• C12M43/00

  Combinations of bioreactors or fermenters with other apparatus · CPC title