Hollow silica nanospheres and methods of making same

Patent metadata

Field	Value
Publication number	US-2016346404-A1
Application number	US-201514981748-A
Country	US
Kind code	A1
Filing date	Dec 28, 2015
Priority date	Aug 14, 2007
Publication date	Dec 1, 2016
Grant date	—

Abstract

The disclosure provide hollow nanospheres and methods of making and using the same. The methods and compositions of the disclosure are useful for drug delivery and gene transfer.

First claim

1 . A method comprising:
(a) depositing a silica-shell precursor on a polyamino acid or carboxylate functionalized template particle under neutral condition to give core-shell spheres; (b) removing the template particle by calcination or organic solvent to provide a hollow silica sphere.

2 . The method of claim 1 , wherein the silica-shell precursor is synthesized by hydrolyzing a silicon-containing compound.

3 . (canceled)

4 . The method of claim 2 , wherein the template comprises an iron or magnetic metal.

5 . The method of claim 1 , wherein the silica-shell precursor comprises a silica-iron ethoxide.

6 . The method of claim 1 , wherein the calcination comprises heating to about 450° C.

7 . The method of claim 1 , wherein the template particle comprises amine-functionalized polystyrene beads.

8 . The method of claim 1 , wherein the precursor of silica shell is deposited on the surface of an amine or carboxylate functionalized polystyrene or latex bead.

9 . The method of claim 1 , wherein the size of the template particle is from about 10 nm to 1 μm.

10 . The method of claim 2 , wherein the silicon-containing compound is selected from the group consisting of tetraalkoxysilanes, trialkoxysilanes, dialkoxysilanes, tetrapropoxysilane, tetraethoxysilane, tetramethoxysilane and any combination thereof.

11 . The method of claim 2 , wherein the silicon-containing compound is selected from the group consisting of tetrapropoxysilane, tetraethoxysilane, tetramethoxysilane and any combination thereof.

12 . The method of claim 2 , wherein the silicon-containing compound is hydrolyzed in an acidic solution.

13 . The method of claim 12 , wherein the acidic solution comprises hydrochloric acid, sulfuric acid, nitric acid or any combination thereof.

14 . The method of claim 12 , wherein the silicon-containing compound is hydrolyzed in an aqueous solution comprising about 0.01M hydrochloric acid.

15 . (canceled)

16 . (canceled)

17 . The method of claim 1 , wherein the polyamino acids comprise monopolymers of amino acids with primary amine groups on the backbone in solid or aqueous solution.

18 . The method of claim 1 , wherein the polyamino acids are about 0.1% v/w aqueous solution of poly-L-lysine, poly-L-arginine and polyornithine.

19 . The method of claim 8 , wherein a silica shell deposits on a surface of polystyrene beads comprising a polyamino acids.

20 - 23 . (canceled)

24 . The method of claim 7 , wherein the polystyrene template is removed by heating in air at about 400-900° C. for 3-6 hours.

25 . (canceled)

26 . The method of claim 7 , wherein the polystyrene template is removed by washing the template-shell spheres in organic solvents selected from the group consisting of toluene, dichloromethane, chloroform, tetrahydrofuran, dimethylformamide, and any combination thereof.

27 . (canceled)

28 . A hollow sphere, comprising:
core-shell spheres fabricated by depositing a silica-shell precursor on a polyamino acid or carboxylate functionalized template particle under neutral condition; wherein the template particle are removed by calcination or organic solvent to provide the hollow sphere.

29 . A hollow sphere of claim 28 , comprising a surface amino group or adsorbed polyamines as carriers of an oligonucleotide or polynucleotide.

30 - 48 . (canceled)

Assignees

• Univ California

Inventors

• Trogler William C
• Esener Sadik C
• Messmer Davorka
• Lind Johan Ulrik
• Mitchell Kristina K P
• Yang Jian

Classifications

• A61K2039/6093

  Synthetic polymers, e.g. polyethyleneglycol [PEG], Polymers or copolymers of (D) glutamate and (D) lysine · CPC title

• C12N15/87

  Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation · CPC title

• A61K47/6923 Primary

  the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb · CPC title

• A61K9/0019

  Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00) · CPC title

• A61P35/00

  Antineoplastic agents · CPC title