Plants with increased fruit size

Patent metadata

Field	Value
Publication number	US-2016333368-A1
Application number	US-201615222667-A
Country	US
Kind code	A1
Filing date	Jul 28, 2016
Priority date	May 28, 2010
Publication date	Nov 17, 2016
Grant date	—

Abstract

The present invention relates to the field of transgenic and non-transgenic plants with novel phenotypes. Provided are Solanum lycopersicum Auxin Response Factor 9 (SlARF9) proteins and nucleic acid sequences encoding these, which are useful in conferring novel phenotypes to plants, especially increased fruit size.

First claim

1 . A non-transgenic plant comprising an Auxin Response Factor 9 (slarf9) allele in its genome, whereby an slarf9 allele is an allele which encodes a protein comprising at least 60% amino acid sequence identity to SEQ ID NO: 2, characterized in that said slarf9 allele comprises one or more mutations in its nucleotide sequence and whereby, as a result of said one or more mutations, the plant comprising said mutant allele in its genome produces significantly larger fruits compared to the plant comprising a wild type SlARF9 allele in its genome.

2 . The plant according to claim 1 , characterized in that said one or more mutations confer loss-of-function or reduced-function onto the encoded SlARF9 protein.

3 . The plant according to claim 1 or 2 , wherein said mutant slarf9 allele is present in homozygous form.

4 . The plant according to any one of the preceding claims, wherein said plant is of the family Solanaceae, of the genus Solanum , or of the species Solanum lycopersicum.

5 . The plant according to any one of the preceding claims, wherein average fruit size is at least 110% of the fruit size of the plant comprising the wild type SlARF9 allele.

6 . The plant according to any one of the preceding claims, wherein the plant is a hybrid plant.

7 . A fruit or seed or a part of a plant according to any one of the preceding claims and comprising in its genome said mutant slarf9 allele.

8 . Use of a nucleic acid sequence encoding an SlARF9 protein for the generation of transgenic or non-transgenic plants which produce large fruits, characterized in that the SlARF9 protein comprises at least 60% amino acid sequence identity to SEQ ID NO: 2.

9 . Use of a nucleic acid sequence encoding an SlARF9 protein for increasing fruit size in plants characterized in that the SlARF9 protein comprises at least 60% amino acid sequence identity to SEQ ID NO: 2.

10 . A transgenic plant comprising integrated in its genome a chimeric gene, wherein said chimeric gene comprises a promoter active in plant cells operably linked to a nucleic acid sequence comprising a sense and/or antisense sequence of a SlARF9 gene which upon transcription silences endogenous SlARF9 gene expression and wherein said plant produces larger fruit compared to a plant lacking said chimeric gene.

11 . The plant according to claim 10 , wherein said endogenous SlARF9 gene encodes a protein comprising at least 60% amino acid sequence identity to SEQ ID NO: 2.

12 . The plant according to claim 10 or 11 , wherein said transcription regulatory sequence is selected from the group consisting of: a constitutive promoter, an inducible promoter, a tissue-specific promoter and a developmentally regulated promoter.

13 . The plant according to any one of claims 10 - 12 , wherein the plant is selected from a genus of the group consisting of: Solanum, Cucumis, Citrullus, Capsicum, Malus, Zea, Oryza, Triticum, Hordeum, Avena and Sorghum.

14 . A seed or a fruit or part of a plant according to any one of claims 10 - 13 comprising said chimeric gene.

15 . A chimeric gene comprising a constitutive-, tissue specific-, inducible- or developmentally regulated promoter active in plant cells, operably linked to a nucleic acid sequence comprising a sense and/or antisense fragment of a SlARF9 gene which upon transcription silences endogenous SlARF9 gene, characterized in that said endogenous SlARF9 gene comprises at least 70% sequence identity to SEQ ID NO: 1 or SEQ ID NO:3 from nucleotide 2005 to 5879.

16 . A non-transgenic tomato plant, or seeds, progeny or tomato fruits thereof, said plant being obtainable by TILLING, comprising a mutant slarf9 allele in its genome, characterized that said mutant allele encodes an SlARF9 protein having reduced-function or loss-of-function compared to the wild type SlARF9 protein.

Assignees

• Nunhems Bv

Inventors

• Vriezen Hendrik Willem
• Mariani Celestina
• De Jong Maaike

Classifications

• A01H5/08

  Fruits · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8222

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8294

  Auxins · CPC title