Method for promoting generation of stem cell-derived exosome by using thrombin

Patent metadata

Field	Value
Publication number	US-2016333317-A1
Application number	US-201415103726-A
Country	US
Kind code	A1
Filing date	Dec 12, 2014
Priority date	Dec 12, 2013
Publication date	Nov 17, 2016
Grant date	—

Abstract

The present invention relates to a method of promoting generation of exosomes from stem cell by using thrombin. The method according to the present invention has superior effects of promoting generation of stem cell-derived exosomes and thus exosomes can be more efficiently obtained thereby compared to conventionally known methods. In addition, the method can be useful for related research.

First claim

1 . A method of promoting generation of an exosome from a stem cell, the method comprising:
culturing a stem cell in a medium comprising thrombin.

2 . The method according to claim 1 , wherein the stem cell is an embryonic stem cell or an adult stem cell.

3 . The method according to claim 2 , wherein the adult stem cell comprises one or more adult stem cell types selected from the group consisting of mesenchymal stem cell, human tissue-derived mesenchymal stromal cell, human tissue-derived mesenchymal stem cell, multipotent stem cell, and amniotic epithelial cell.

4 . The method according to claim 3 , wherein the mesenchymal stem cells are derived from one or more tissues selected from the group consisting of umbilical umbilical cord, umbilical cord blood, bone marrow, fat, muscle, nerve, skin, amnion, and placenta.

5 . The method according to claim 1 , wherein the medium comprises one or more selected from the group consisting of Dulbecco's Modified Eagle's Medium (DMEM), Minimal Essential Medium (MEM), Basal Medium Eagle (BME), RPMI 1640, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-10 (DMEM/F-10), Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 (DMEM/F-12), α-Minimal essential Medium (α-MEM), Glasgow's Minimal Essential Medium (G-MEM), Isocove's Modified Dulbecco's Medium (IMDM), and KnockOut DMEM.

6 . The method according to claim 1 wherein the medium comprises the thrombin at a concentration of 1 to 1000 U/ml.

7 . The method according to claim 1 , wherein the culturing is performed for 12 to 48 hours.

8 . (canceled)

9 . A method of increasing expression of a growth factor in an exosome derived from a stem cell, the method comprising:
culturing a stem cell in a medium comprising thrombin.

10 . The method according to claim 9 , wherein the growth factor comprises one or more selected from the group consisting of brain-derived neurotropic factor (BDNF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), nerve growth factor (NGF), and vascular endothelial growth factor (VEGF).

11 . A method for promoting generation of exosomes from a stem cell, comprising:
providing a medium composition comprising thrombin for exosome generation.

Assignees

• Samsung Life Public Welfare Foundation

Inventors

• Chang Yun Sil
• Park Won Soon
• Sung Dong Kyung
• Ahn So Yoon

Classifications

• C12N5/00 Primary

  Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) · CPC title

• A61K35/51

  Umbilical cord; Umbilical cord blood; Umbilical stem cells · CPC title

• A61K35/545

  Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells · CPC title

• C12N5/0665 Primary

  Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood · CPC title

• C12N5/0696

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title