Device for the culturing of microorganisms and associated process

Patent metadata

Field	Value
Publication number	US-2016333299-A1
Application number	US-201515112988-A
Country	US
Kind code	A1
Filing date	Jan 22, 2015
Priority date	Jan 20, 2014
Publication date	Nov 17, 2016
Grant date	—

Abstract

A device for the culturing and/or isolation and/or detection and/or identification and/or counting of at least one target microorganism in a sample liable to contain it, which includes a support and a nutrient medium; the support including: a hydrophilic fibrous substrate, at least one porous layer in contact with one of the faces of the fibrous substrate, including a pigment or a mixture of pigments and at least one binder, the pigment having a size of less than 5 μm and the amount of the pigment or the mixture of pigments being between 50 and 97% by dry weight, with respect to the dry weight of the porous layer; the nutrient medium being included in the fibrous substrate. Also relates to the use and to the process for the manufacture of the device.

First claim

1 . A device for the culturing and/or isolation and/or detection and/or identification and/or counting of at least one target microorganism in a sample liable to contain it, wherein it comprises a support and a nutrient medium;
said support comprising:
a hydrophilic fibrous substrate,
at least one porous layer in contact with one of the faces of the fibrous substrate, comprising a pigment or a mixture of pigments and at least one binder, said pigment having a size of less than 5 μm and the amount of said pigment or said mixture of pigments being between 50 and 97% by dry weight, with respect to the dry weight of the porous layer;
said nutrient medium being included in the fibrous substrate.

2 . The device as claimed in claim 1 , wherein the porous layer is integral with one of the faces of the fibrous substrate.

3 . The device as claimed in claim 1 , wherein the pigments are inorganic pigments chosen from the following pigments: kaolin, talc, titanium dioxide and calcium carbonate.

4 . The device as claimed in claim 1 , wherein the binder is chosen from the following binders: styrene-butadiene latex, styrene-acrylic latex and carboxymethyl cellulose.

5 . The device as claimed in claim 1 , wherein the amount of binder is between 3 and 25% by dry weight, with respect to the dry weight of the porous layer.

6 . The device as claimed in claim 1 , comprising:
a first porous layer, in contact with the fibrous substrate, containing titanium dioxide, and a second external porous layer comprising kaolin.

7 . The device as claimed in claim 1 , wherein the fibrous substrate comprises cellulose fibers.

8 . The device as claimed in claim 1 , wherein the nutrient medium is a dehydrated medium.

9 . The device as claimed in claim 1 , wherein the porous layer comprises a crosslinking agent.

10 . The device as claimed in claim 9 , wherein the crosslinking agent is chosen from the following crosslinking agents: isocyanate and melamine-formaldehyde.

11 . A process for the manufacture of a support comprising a stage of deposition by coating, on one of the faces of a hydrophilic fibrous substrate, of a porous layer comprising a pigment or a mixture of pigments and at least one binder, said pigment having a size of less than 5 μm and the amount of said pigment or of the mixture of pigments being between 50 and 97% by dry weight, with respect to the dry weight of the porous layer.

12 . The process for the manufacture of a device as claimed in claim 11 , comprising a stage, prior or subsequent to the coating stage, of bringing the hydrophilic fibrous substrate into contact with a nutrient medium.

13 . A product obtained by the process as claimed in claim 11 .

14 . A support intended for the growth of microorganisms comprising:
a hydrophilic fibrous substrate; at least one porous layer deposited on one of the faces of the fibrous substrate, comprising a pigment or a mixture of pigments and at least one binder, said pigment having a size of less than 5 μm and the amount of said pigment or of the mixture of pigments being between 50 and 97% by dry weight, with respect to the dry weight of the porous layer.

15 . (canceled)

16 . A process for the culturing and/or isolation and/or detection and/or identification and/or counting of at least one target microorganism, in a sample liable to contain it, said process comprising the following stages:
a) providing a device as claimed in claim 1 , b) depositing a predetermined volume of the sample on the porous layer, c) incubating the device for a predetermined time and at a predetermined temperature which make possible the growth and the appearance of colonies of at least one target microorganism, d) detecting and/or identifying and/or counting the colonies formed; said process also comprising at least one stage of rehydration of the culture medium with a predetermined volume of liquid, before or simultaneously with stage b) and/or c).

17 . The process as claimed in claim 16 , wherein a device, in which the porous layer comprises a crosslinking agent, is provided and that, after the deposition of the sample, the microorganisms are isolated using an isolation means.

18 . The process as claimed in claim 16 , wherein the identification takes place by spectral analysis.

Assignees

• Biomerieux Sa
• Arjo Wiggins Fine Papers Ltd

Inventors

• Montet Marie-Pierre
• Rozand Christine
• Depres Gael
• Vau Jean-Marie
• Baumlin Jean-Marie

Classifications

• C12M25/14

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• B05D1/28

  performed by transfer from the surfaces of elements carrying the liquid or other fluent material, e.g. brushes, pads, rollers · CPC title

• C12M23/20 Primary

  Material Coatings (immunocoatings C12M25/00) · CPC title