Selectively targeted antimicrobial peptides and the use thereof

Patent metadata

Field	Value
Publication number	US-2016331804-A1
Application number	US-201615163334-A
Country	US
Kind code	A1
Filing date	May 24, 2016
Priority date	Sep 6, 2006
Publication date	Nov 17, 2016
Grant date	—

Abstract

The present invention relates to targeting peptides capable of specifically binding to microbial organisms (e.g., P. aeruginosa or S. mutans ), antimicrobial peptides having antimicrobial activities, and specifically/selectively targeted antimicrobial peptides (STAMPs). In addition, the present invention provides methods of selectively killing or inhibiting microbial organisms by using the peptides or compositions provided by the present invention.

First claim

1 - 27 . (canceled)

28 . A method of reducing the ratio of living Streptococcus mutans to total streptococci in a biofilm, said method comprising contacting said S. mutans with an amount of a construct effective to kill an S. mutans , wherein said construct comprises a targeting peptide that binds S. mutans attached to an antimicrobial peptide, wherein the amino acid sequence of said targeting peptide consists of a fragment of the competence stimulating peptide (CSP) ranging in length from 8 to 20 amino acids.

29 . The method of claim 28 , wherein said fragment comprises the amino acid sequence TFFRLFNR (SEQ ID NO:5).

30 . The method of claim 28 , wherein said fragment comprises the amino acid sequence TFFRLFNRSFTQALGK.

31 . The method of claim 28 , wherein said fragment consists of the amino acid sequence TFFRLFNRSFTQALGK.

32 . The method according to any one of claims 28 - 31 , wherein the amino acid sequence of said antimicrobial peptide comprises a novispirin amino acid sequence or a fragment thereof having antimicrobial activity.

33 . The method of claim 32 , wherein the amino acid sequence of said antimicrobial peptide comprises the sequence KNLRIIRKGIHIIKKY (SEQ ID NO:3).

34 . The method of claim 32 , wherein the amino acid sequence of said antimicrobial peptide consists of the sequence KNLRIIRKGIHIIKKY (SEQ ID NO:3).

35 . The method of claim 32 , wherein the targeting peptide is attached to the antimicrobial peptide by a linker peptide.

36 . The method of claim 35 , wherein the targeting peptide is attached to the antimicrobial peptide by a linker peptide, where the amino acid sequence of said linker peptide is selected from the group consisting of GGG (SEQ ID NO:17), AAA (SEQ ID NO:18), SAT (SEQ ID NO:19), ASA (SEQ ID NO:20), SGG (SEQ ID NO: 21), PYP (SEQ ID NO:22), SGS (SEQ ID NO:23), GGS (SEQ ID NO:24), SPS(SEQ ID NO:25), PSGSP (SEQ ID NO:26), PSPSP (SEQ ID NO:27), and GGSGGS (SEQ ID NO:28).

37 . The method of claim 32 , wherein the amino acid sequence of said construct comprises the sequence TFFRLFNRSFTQALGKGGGKNLRIIRKGIHIIKKY.

38 . The method of claim 32 , wherein the amino acid sequence of said construct consists of the sequence TFFRLFNRSFTQALGKGGGKNLRIIRKGIHIIKKY.

39 . The method of claim 38 , wherein the carboxyl terminus of said antimicrobial peptide is amidated.

40 . The method of claim 38 , wherein said biofilm is on an oral soft tissue or on teeth.

41 . The method of claim 32 , wherein the carboxyl terminus of said antimicrobial peptide is amidated.

42 . The method of claim 40 , wherein said biofilm is on an oral soft tissue or on teeth.

Assignees

• Univ California
• C3 Jian Inc

Inventors

• Eckert Randal H
• Yarbrough Daniel K
• Shi Wenyuan
• Anderson Maxwell H
• Qi Fengxia
• He Jian
• Mchardy Ian H

Classifications

• C07K14/001

  by chemical synthesis · CPC title

• A61K47/65

  Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• C07K2319/01

  containing a localisation/targetting motif · CPC title

• C07K14/315

  from Streptococcus (G), e.g. Enterococci · CPC title