Composition, method for preparing sugar chain sample, and method for analyzing sugar chain

Patent metadata

Field	Value
Publication number	US-2016327568-A1
Application number	US-201515109309-A
Country	US
Kind code	A1
Filing date	Jan 13, 2015
Priority date	Jan 13, 2015
Publication date	Nov 10, 2016
Grant date	—

Abstract

A composition is provided, including: polymer particles having a functional group for capturing a sugar chain, wherein the rate of change of mass calculated by Equation (F1) below is more than 90% and less than 112%.
Rate of change (%)= A/B ×100  (F1)

In Equation (F1), A represents the mass of the composition after incubation for 4 hours under an environment of a relative humidity of 50% and a temperature of 25° C., and B represents the mass of the composition before the incubation.

First claim

1 . A composition, comprising:
polymer particles having a functional group which captures a sugar chain, wherein the composition has a rate of change of mass in a range of more than 90% to less than 112%, and the rate of change of mass is represented by Equation (F1), rate=A/B×100, where A represents a mass of the composition after incubation for 4 hours under an environment of a relative humidity of 50% and a temperature of 25° C., and B represents a mass of the composition before the incubation.

2 . The composition according to claim 1 , wherein the composition has a rate of change of average particle diameter in a range of 90% to 120%, where the rate of change of average particle diameter is represented by Equation (F2), rate=C/D×100, where C represents an average particle diameter of the polymer particles in the composition, measured by a laser diffraction/scattering particle size analyzer after heat-retaining treatment of the composition at 60° C. for 3 days under a drying condition and suspending the composition in water, and D represents an average particle diameter of the polymer particles in the composition, measured by a laser diffraction/scattering particle size analyzer before the heat-retaining treatment of the composition and suspending the composition in water.

3 . The composition according to claim 1 , further comprising:
a surfactant.

4 . The composition according to claim 3 , wherein the polymer particles are coated with the surfactant.

5 . The composition according to claim 3 , wherein the surfactant is a sorbitan fatty acid ester or an anionic surfactant.

6 . The composition according to claim 1 , wherein an average particle diameter of the polymer particles is in a range of 0.05 μm to 1000 μm.

7 . The composition according to claim 1 , wherein the functional group is a hydrazide group or an aminooxy group.

8 . The composition according to claim 1 , wherein each of the polymer particles has a crosslinked polymer structure represented by Formula (1)

where each of R 1 and R 2 represents a hydrocarbon chain of 1 to 20 carbon atoms which is optionally interrupted by —O—, —S—, —NH—, —CO—, or —CONH—; each of R 3 , R 4 , and R 5 represents H, CH 3 , or a hydrocarbon chain of 2 to 5 carbon atoms; and m:n=99:1 to 70:30 is satisfied.

9 . The composition according to claim 8 , wherein the crosslinked polymer structure is represented by Formula (2)

where m:n=99:1 to 70:30 is satisfied.

10 . A method for preparing a sugar chain sample, comprising:
mixing a sample comprising a sugar chain with the composition according to claim 1 to bond the sugar chain to the polymer particles in the composition; washing the polymer particles bonded to the sugar chain; and liberating the sugar chain from the polymer particles bonded to the sugar chain.

11 . A method for analyzing a sugar chain in a sample, comprising:
mixing a sample comprising a sugar chain with the composition according to claim 1 to bond the sugar chain to the polymer particles in the composition; washing the polymer particles bonded to the sugar chain; liberating the sugar chain from the polymer particles bonded to the sugar chain; and analyzing the liberated sugar chain.

12 . The composition according to claim 2 , further comprising:
a surfactant.

13 . The composition according to claim 12 , wherein the polymer particles are coated with the surfactant.

14 . The composition according to claim 4 , wherein the surfactant is a sorbitan fatty acid ester or an anionic surfactant.

15 . The composition according to claim 2 , wherein an average particle diameter of the polymer particles is in a range of 0.05 μm to 1000 μm.

16 . The composition according to claim 2 , wherein the functional group is a hydrazide group or an aminooxy group.

17 . The composition according to claim 2 , wherein each of the polymer particles has a crosslinked polymer structure represented by Formula (1)

where each of R 1 and R 2 represents a hydrocarbon chain of 1 to 20 carbon atoms which is optionally interrupted by —O—, —S—, —NH—, —CO—, or —CONH—; each of R 3 , R 4 , and R 5 represents H, CH 3 , or a hydrocarbon chain of 2 to 5 carbon atoms; and m:n=99:1 to 70:30 is satisfied.

18 . The composition according to claim 17 , wherein the crosslinked polymer structure is represented by Formula (2)

where m:n=99:1 to 70:30 is satisfied.

19 . The composition according to claim 3 , wherein an average particle diameter of the polymer particles is in a range of 0.05 μm to 1000 μm.

20 . The composition according to claim 3 , wherein the functional group is a hydrazide group or an aminooxy group.

Assignees

• Sumitomo Bakelite Co

Inventors

• Matsumoto Takayuki

Classifications

• B01J20/321 Primary

  consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds · CPC title

• B01J20/264

  derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers · CPC title

• B01J20/3293

  Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers · CPC title

• B01J20/3248

  the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such · CPC title

• G01N1/34

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title