Alleles modifying brassica plant total saturated fatty acid content

Patent metadata

Field	Value
Publication number	US-2016309672-A1
Application number	US-201415037887-A
Country	US
Kind code	A1
Filing date	Nov 21, 2014
Priority date	Nov 21, 2013
Publication date	Oct 27, 2016
Grant date	—

Abstract

The present disclosure sets forth alleles at two genetic loci whose presence reduces the saturated fatty acid content of Brassica seeds. Methods for producing plants containing those alleles, and which produce seeds having low saturated fatty acid content, are also described.

First claim

What is claimed is:

1 - 28 . (canceled)

29 . A Brassica plant that is non-transgenic or a Brassica plant that is free of transgenes other than those for herbicide tolerance, or part thereof, comprising a nucleic acid sequence having greater than 90%, 95%, 97.5%, 98%, 99%, 99.9%, 99.99%, or 99.999% identity, or having 100% identity to all of, comprising all of, or a part comprising greater than 20, 30, 40, 50 or 60 contiguous nucleotides of, the genomic sequences between the chromosome N1 (QTL1) SNP markers at positions 20772548 and 22780181, 20843387 and 21080816, or 20874571 and 20979545 of the B. napus Salomon line ATCC deposit designation PTA-11453; said plant further comprising mutant alleles at two or more, three or more, or four or more, different fatty acyl ACP thioesterase B (FATB) loci, wherein each said mutant allele results in the production of a FATB polypeptide having reduced thioesterase activity relative to a corresponding wild-type FATB polypeptide.

30 . The Brassica plant, or a part thereof, of claim 29 , wherein at least one of said mutant alleles comprises a nucleic acid encoding a truncated FATB polypeptide.

31 . The Brassica plant, or a part thereof, of claim 29 , wherein at least one of said mutant alleles comprises a nucleic acid encoding a FATB polypeptide having a deletion of a helix/4-stranded sheet (4HBT) domain or a portion thereof.

32 . The Brassica plant, or a part thereof, of claim 29 , wherein at least one of said mutant alleles comprises a nucleic acid encoding a FATB polypeptide having a non-conservative substitution of a residue affecting substrate specificity.

33 . The Brassica plant, or a part thereof, of claim 29 , wherein at least one of said mutant alleles comprises a nucleic acid encoding a FATB polypeptide having a non-conservative substitution of a residue affecting catalytic activity.

34 . The Brassica plant, or a part thereof, of claim 29 , wherein said plant produces seeds yielding an oil having a total saturates content of about 2.5% to 5.5%.

35 . The Brassica plant, or a part thereof, of claim 34 , said oil further having an oleic acid content of about 78% to 80%, a linoleic acid content of about 8% to 10%, and an α-linolenic acid content of about 2% to 4%.

36 . The Brassica plant, or a part thereof, of claim 29 , wherein said plant produces seeds yielding an oil having a palmitic acid content of about 1.5% to 3.5%.

37 . The Brassica plant, or a part thereof, of claim 29 , wherein said plant produces seeds yielding an oil having a stearic acid content of about 0.5% to 2.5%.

38 . The Brassica plant, or a part thereof of claim 29 , said plant further comprising a mutant allele at a delta-12 fatty acid desaturase (FAD2) locus, said mutant allele comprising a nucleic acid encoding a FAD2 polypeptide having a lysine substituted for glutamic acid in a His-Glu-Cys-Gly-His motif.

39 . The Brassica plant, or a part thereof, of claim 38 , said plant further comprising a mutant allele at a different FAD2 locus, said mutant allele comprising a nucleic acid encoding a FAD2 polypeptide having a glutamic acid substituted for glycine in the DRDYGILNKV motif or a histidine substituted for leucine in a KYLNNP motif.

40 . The Brassica plant, or a part thereof, of claim 29 , said plant further comprising a mutant allele at a FAD2 locus, said mutant allele comprising a nucleic acid encoding a FAD2 polypeptide having a glutamic acid substituted for glycine in the DRDYGILNKV motif or a histidine substituted for leucine in a KYLNNP motif.

41 - 92 . (canceled)

Assignees

• Cargill Inc

Inventors

• Fletcher Richard
• Herrmann David
• Zheng Honggang

Classifications

• C12Y301/02014

  Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N9/16

  acting on ester bonds (3.1) · CPC title

• C12Y114/19003

  Linoleoyl-CoA desaturase (1.14.19.3) · CPC title

• A01H5/10 Primary

  Seeds · CPC title