Pertussis antibodies and uses thereof

Patent metadata

Field	Value
Publication number	US-2016304588-A1
Application number	US-201614990619-A
Country	US
Kind code	A1
Filing date	Jan 7, 2016
Priority date	Sep 17, 2010
Publication date	Oct 20, 2016
Grant date	—

Abstract

Compositions and methods are provided that are useful to treat respiratory diseases such as whooping cough. Further, compositions and methods of immunizing are provided.

First claim

1 .- 65 . (canceled)

66 . A humanized 1B7 antibody comprising a humanized light chain variable region and a humanized heavy chain variable region,
wherein said humanized light chain variable region comprises:
(i) a mouse CDR L1 as set forth in SEQ ID NO:3, a mouse CDR L2 as set forth in SEQ ID NO:4, a mouse CDR L5 as set forth in SEQ ID NO:5 and
(ii) a tyrosine at a position corresponding to Kabat position 71, a serine at a position corresponding to Kabat position 10, a leucine at a position corresponding to Kabat position 11, a glycine at a position corresponding to Kabat position 16, an arginine at a position corresponding to Kabat position 18, a glycine at a position corresponding to Kabat position 41, a lysine at a position corresponding to Kabat position 42, or a leucine at a position corresponding to Kabat position 47; and
wherein said humanized heavy chain variable region comprises:
(iii) a mouse CDR H1 as set forth in SEQ ID NO:6, a mouse CDR H2 as set forth in SEQ ID NO:7, and a mouse CDR H3 as set forth in SEQ ID NO:8 and
(iv) a valine at a position corresponding to Kabat position 71, an arginine at a position corresponding to Kabat position 38, an alanine at a position corresponding to Kabat position 40, or a valine at a position corresponding to Kabat position 109.

67 . The humanized 1B7 antibody of claim 66 , wherein said humanized light chain variable region comprises a tyrosine at a position corresponding to Kabat position 71 and said humanized heavy chain variable region comprises a valine at a position corresponding to Kabat position 71.

68 . The humanized 1B7 antibody of claim 66 , wherein said antibody is a Fab′ fragment.

69 . The humanized 1B7 antibody of claim 66 , wherein said antibody comprises a human constant region.

70 . The humanized 1B7 antibody of claim 66 , wherein said antibody is an IgG.

71 . The humanized 1B7 antibody of claim 66 , wherein said antibody is a single chain antibody (scFv).

72 . The humanized 1B7 antibody of claim 66 , wherein said antibody is capable of binding a pertussis toxin protein with an equilibrium dissociation constant (K D ) less than 10 nM.

73 . The humanized 1B7 antibody of claim 72 , wherein said pertussis toxin protein is a truncated pertussis toxin protein.

74 . The humanized 1B7 antibody of claim 73 , wherein said truncated pertussis toxin protein comprises the amino acid sequence corresponding to amino acid residues 1 to 220 of SEQ ID NO:9.

75 . The humanized 1B7 antibody of claim 66 , wherein said humanized antibody is an antibody which competes with an antibody that is capable of binding a pertussis toxin protein comprising the amino acid sequence corresponding to amino acid residues 1 to 220 of SEQ ID NO:9.

76 . A humanized antibody which binds the same epitope as the antibody set forth in claim 66 .

77 . A pharmaceutical composition comprising a therapeutically effective amount of a humanized antibody as set forth in claim 66 .

78 . An isolated nucleic acid encoding a humanized antibody as set forth in claim 66 .

79 . A method of treating whooping cough in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a humanized antibody as set forth in claim 66 and a pharmaceutically acceptable excipient, thereby treating whooping cough in the subject.

Assignees

• Univ Texas

Inventors

• Maynard Jennifer A
• Sutherland Jamie

Classifications

• C07K2317/622

  Single chain antibody (scFv) · CPC title

• C07K2317/94

  Stability, e.g. half-life, pH, temperature or enzyme-resistance · CPC title

• C07K16/1225 Primary

  from Bordetella (G) · CPC title

• C07K2317/24

  containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered · CPC title

• C07K2317/55

  Fab or Fab' · CPC title