De-Beading

Patent metadata

Field	Value
Publication number	US-2016296943-A1
Application number	US-201615093597-A
Country	US
Kind code	A1
Filing date	Apr 7, 2016
Priority date	Apr 7, 2015
Publication date	Oct 13, 2016
Grant date	—

Abstract

Embodiments relate to systems and methods for removing beads from fluid that may include cells as well as the beads. Embodiments may utilize one or more: separation chambers, flow cells, magnets, and/or magnetic fields to remove beads from a fluid stream.

First claim

What is claimed is:

1 . A system for removing magnetic beads from a fluid stream comprising cells, the system comprising:
a fluid pathway for flowing the fluid stream, wherein the fluid pathway comprises an indirect pathway in a bag; a magnetic field adjacent the bag; and a pump for moving fluid through the fluid pathway.

2 . The system of claim 1 , wherein the magnetic field is generated by a plurality of magnets.

3 . The system of claim 1 , wherein the indirect pathway comprises a channel in a serpentine bag.

4 . The system of claim 1 , wherein the bag comprises barriers around which the fluid stream flows.

5 . The system of claim 1 , wherein the magnetic beads comprise iron and are attracted to a wall of the bag by the magnetic field.

6 . The system of claim 5 , wherein the magnetic field is strong enough to maintain the magnetic beads against the wall.

7 . The system of claim 6 , wherein the indirect pathway of the bag is long enough to allow the magnetic field to remove at least about ninety percent of the magnetic beads from the fluid stream.

8 . The system of claim 7 , wherein the pump moves fluid into the bag at a rate greater than about 10 ml/min.

9 . The system of claim 8 , wherein the outlet of the bag is in fluid communication with a chamber for concentrating the cells in the fluid stream after the magnetic beads are removed from the fluid stream.

10 . The system of claim 9 , wherein the chamber removes liquid and additional magnetic beads from the fluid stream.

11 . A method of removing magnetic beads from a fluid stream comprising cells, the method comprising:
moving the fluid stream through a fluid pathway comprising an indirect pathway in a bag, wherein the moving comprises pumping the fluid stream; subjecting the fluid stream to a magnetic field while in the bag; and removing at least about eighty five percent of the magnetic beads from the fluid stream while the stream flows through the bag.

12 . The method of claim 11 , wherein the magnetic field is generated by a plurality of magnets.

13 . The method of claim 11 , wherein the plurality of magnets comprise an array with alternating poles.

14 . The method of claim 11 , wherein the indirect path comprises a channel in a serpentine bag.

15 . The method of claim 11 , wherein the magnetic beads comprise iron and are attracted to a wall of the serpentine bag by the magnetic field.

16 . The method of claim 15 , wherein the magnetic field is strong enough to maintain the magnetic beads against the wall.

17 . The method of claim 16 , wherein the indirect path of the bag is long enough to allow the magnetic field to remove at least about ninety percent of the magnetic beads from the fluid stream.

18 . The method of claim 17 , moving the fluid into the bag at a rate of greater than about 20 ml/min.

19 . The method of claim 18 , moving the fluid stream after the magnetic beads are removed from the outlet of the bag to a chamber for concentrating the cells.

20 . The method of claim 19 , removing liquid and additional magnetic beads from the fluid stream using the chamber.

Assignees

• Terumo Bct Inc

Inventors

• Ladtkow Tanner J
• Ellingboe Bruce
• Scholler Jennifer D
• Pittinger John J

Classifications

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• A61M1/3693

  using separation based on different densities of components, e.g. centrifuging · CPC title

• B03C1/02 Primary

  acting directly on the substance being separated · CPC title

• A61M1/3692 Primary

  Washing or rinsing blood or blood constituents · CPC title