Electrode system for measuring an analyte concentration under in-vivo conditions

Patent metadata

Field	Value
Publication number	US-2016296151-A1
Application number	US-201615185158-A
Country	US
Kind code	A1
Filing date	Jun 17, 2016
Priority date	Sep 11, 2008
Publication date	Oct 13, 2016
Grant date	—

Abstract

Electrode systems are disclosed for measuring the concentration of an analyte under in-vivo conditions, where such systems include a counter electrode having an electrical conductor, a working electrode having an electrical conductor on which an enzyme layer containing immobilized enzyme molecules for catalytic conversion of the analyte is arranged, and a diffusion barrier that slows the diffusion of the analyte from body fluid surrounding the electrode system to enzyme molecules. The enzyme layer is in the form of multiple fields that are arranged on the conductor of the working electrode at a distance from each other.

First claim

What is claimed is:

1 . An electrode system for measuring an analyte concentration under in-vivo conditions, the system comprising:
a counter electrode having an electrical conductor; a working electrode having an electrical conductor on which an enzyme layer containing immobilized enzyme molecules for catalytic conversion of the analyte is arranged, wherein the enzyme layer is in the form of multiple fields that are arranged on the conductor of the working electrode at a pre-determined distance from each other; and a diffusion barrier that slows the diffusion of the analyte from body fluid surrounding the electrode system to the enzyme molecules.

2 . The electrode system of claim 1 , further comprising a substrate wherein the electrical conductor of the working electrode and the electrical conductor of the counter electrode are arranged on the substrate.

3 . The electrode system of claim 1 , wherein the multiple fields are arranged in one or more rows or series.

4 . The electrode system of claim 3 , wherein the multiple fields are arranged at a pre-determined distance from one another on the electrical conductor of the working electrode so that the multiple fields are separate from each other.

5 . The electrode system of claim 1 , wherein between the fields of the enzyme layer, the conductor of the working electrode is covered by an insulation layer.

6 . The electrode system of claim 1 , wherein at least two of the fields of the enzyme layer are at least 3 mm distant from each other.

7 . The electrode system of claim 1 , wherein a distance of at least 0.3 mm exists between neighboring fields of the enzyme layer.

8 . The electrode system of claim 1 , wherein the fields of the enzyme layer each extend less than 2 mm in two directions that are perpendicular to each other.

9 . The electrode system of claim 1 , wherein the diffusion barrier is a layer covering the enzyme layer.

10 . The electrode system of claim 1 , wherein the diffusion barrier is a mixture of at least two different acrylates.

11 . The electrode system of claim 10 , wherein at least one of the acrylates is a copolymer.

12 . The electrode system of claim 11 , wherein the enzyme interacts with a catalytic redox mediator contained in the enzyme layer and reduces or prevents an oxygen dependence of the catalytic conversion of the analyte.

13 . The electrode system of claim 12 , wherein the catalytic redox mediator converts hydrogen peroxide.

14 . The electrode system of claim 13 , wherein the catalytic redox mediator effects a direct electron transfer.

15 . The electrode system of claim 1 , wherein the enzyme layer is covered by a spacer.

16 . The electrode system of claim 1 , wherein the spacer covers the working electrode and the counter electrode in the form of a continuous layer.

17 . The electrode system of claim 1 , wherein the conductor of the working electrode narrows between the enzyme layer fields and the conductor of the counter electrode has a contour that follows the course of the conductor of the working electrode.

18 . A sensor comprising:
an electrode system comprising:
a counter electrode having an electrical conductor;
a working electrode having an electrical conductor on which an enzyme layer containing immobilized enzyme molecules for catalytic conversion of the analyte is arranged, wherein the enzyme layer is in the form of multiple fields that are arranged on the conductor of the working electrode at a pre-determined distance from each other;
a potentiostat connected to the electrode system, and an amplifier for amplifying measuring signals of the electrode system.

19 . The sensor of claim 18 , wherein the working and counter electrodes of the electrode system are arranged on a substrate that carries the potentiostat or are attached on a circuit board that carries the potentiostat.

20 . An electrode system for measuring an analyte concentration under in-vivo conditions, the system comprising:
a counter-electrode having an electrical conductor; a working electrode having an electrical conductor on which an enzyme layer containing immobilized enzyme molecules for catalytic conversion of the analyte is arranged, wherein the enzyme layer is provided over a distance of at least 3 mm in the form of multiple fields that are arranged on the conductor of the working electrode at a pre-determined distance from each other; and a diffusion barrier that slows the diffusion of the analyte from body fluid surrounding the electrode system to the enzyme molecules.

Assignees

• Roche Diabetes Care Inc

Inventors

• Rieger Ewald
• Mischler Reinhold
• Quarder Ortrud
• Staib Arnulf
• Kamacke Ulrike
• Dillen Ralph

Classifications

• A61B5/7207

  of noise induced by motion artifacts · CPC title

• A61B5/14865 Primary

  invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors · CPC title

• A61B5/14532

  for measuring glucose, e.g. by tissue impedance measurement · CPC title

• C12Q1/006

  for glucose · CPC title

• C12Q1/001

  Enzyme electrodes · CPC title