Conductive hydrogels for affinity sensing

Patent metadata

Field	Value
Publication number	US-2016291001-A1
Application number	US-201615186089-A
Country	US
Kind code	A1
Filing date	Jun 17, 2016
Priority date	Dec 19, 2013
Publication date	Oct 6, 2016
Grant date	—

Abstract

The present invention provides a sensor for detection of disease markers, and methods for detecting a disease marker, using a conductive hydrogel modified with a biorecognition element.

First claim

What is claimed is:

1 . A sensor comprising:
a substrate; at least one electrode contacting the substrate; a nanoporous membrane covering the electrode; and a biorecognition element selected from the group consisting of a peptide, an antibody, an enzyme, and an aptamer, wherein the biorecognition element is covalently bound to the electrode, or covalently bound to a PEDOT random copolymer embedded within the nanoporous membrane, the PEDOT random copolymer having a structure according to Formula I:

wherein each R is independently selected from the group consisting of —OH and the biorecognition element, wherein at least one R is the biorecognition element, and
x and y are independently an integer of from about 1 to about 1000, wherein the sum of x and y is an integer of from about 2 to about 1000.

2 . The sensor of claim 1 , wherein the electrode is a micropatterned gold electrode.

3 . The sensor of claim 1 , wherein the sensor comprises a center working electrode, a surrounding counter electrode, and a reference electrode.

4 . The sensor of claim 1 , wherein the biorecognition element is an antibody.

5 . The sensor of claim 1 , wherein the biorecognition element is an aptamer.

6 . The sensor of claim 1 , wherein the biorecognition element is covalently bound to the PEDOT random copolymer embedded within the nanoporous membrane, wherein the nanoporous membrane comprises a PEG hydrogel having PEG chains with a molecular weight of from about 1000 Da to about 10,000 Da.

7 . The sensor of claim 6 , wherein the PEG hydrogel comprises PEG chains having a molecular weight of about 6000 Da.

8 . The sensor of claim 6 , wherein the PEG hydrogel is covalently bound to the substrate.

9 . The sensor of claim 6 , wherein the ratio of x to y is from about 10:1 to about 1:10.

10 . The sensor of claim 1 , wherein the nanoporous membrane is an aluminum oxide membrane.

11 . The sensor of claim 10 , wherein the biorecognition element is covalently linked to the electrode to form a self-assembled monolayer of the biorecognition element on the electrode.

12 . The sensor of claim 11 , wherein the biorecognition element is an aptamer having a redox reporting moiety and a thiol moiety, wherein the thiol moiety is covalently bound to a gold working electrode.

13 . A method for detecting a disease marker in a biological sample comprising contacting a sensor according to claim 1 with the biological sample and detecting the binding of the disease marker to a biorecognition element, thereby detecting the disease marker.

14 . The method of claim 13 , wherein detecting the binding of the disease marker to the biorecognition element comprises measuring the peak reduction current of the PEDOT random copolymer.

15 . The method of claim 13 , wherein the binding of the disease marker to the biorecognition element is detected using square wave voltammetry.

16 . The method of claim 13 , wherein the disease marker is indicative of an infection by tuberculosis or hepatitis C.

17 . A conductive hydrogel comprising:
a covalently cross-linked poly(ethylene glycol) (PEG) hydrogel; and a poly(3,4-ethylenedioxythiophene) (PEDOT) random copolymer embedded within the PEG hydrogel, the PEDOT random copolymer having a structure according to Formula I:

wherein each R is independently selected from the group consisting of —OH and a biorecognition element selected from the group consisting of a peptide, an antibody, an enzyme, and an aptamer, wherein at least one R is the biorecognition element, and
x and y are independently an integer of from about 1 to about 1000, wherein the sum of x and y is an integer of from about 2 to about 1000.

18 . The conductive hydrogel of claim 17 , wherein the PEG hydrogel comprises PEG chains having molecular weights of from about 1000 Da to about 10,000 Da.

19 . The conductive hydrogel of claim 17 , wherein the ratio of x to y is from about 10:1 to about 1:10.

20 . The conductive hydrogel of claim 19 , wherein the covalently cross-linked poly(ethylene glycol) hydrogel is prepared from PEG-diacrylate.

Assignees

• Univ California

Inventors

• Revzin Alexander
• Matharu Zimple
• You Jungmok
• Kwon Lydia
• Liu Ying
• Shin Dong-Sik

Classifications

• G01N33/545

  Synthetic resin · CPC title

• G01N2333/35

  from Mycobacteriaceae (F) · CPC title

• G01N27/3277

  being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48) · CPC title

• G01N2800/26

  Infectious diseases, e.g. generalised sepsis · CPC title

• G01N2333/57

  IFN-gamma · CPC title