Slip Chip Device and Methods

Patent metadata

Field	Value
Publication number	US-2016288121-A1
Application number	US-201615164798-A
Country	US
Kind code	A1
Filing date	May 25, 2016
Priority date	Mar 24, 2009
Publication date	Oct 6, 2016
Grant date	—

Abstract

A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

First claim

1 . An apparatus for determining the quantity of an analyte present in a sample, the apparatus comprising: a top plate comprising a plurality of areas arranged to form a plurality of rows extending parallel to one another; and a bottom plate comprising a plurality of areas arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate; wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the areas of the top plate communicate with the areas of the bottom plate so as to form a plurality of rows; wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels; and wherein the areas in the top plate and the areas in the bottom plate extend at a 45 degree angle relative to the axis of a row.

2 . The apparatus of claim 1 , wherein the top plate is transparent.

3 . The apparatus of claim 1 , wherein at least one of the plurality of rows formed in the top plate comprises an inlet and an outlet.

4 . The apparatus of claim 1 , wherein each of the plurality of channels is connected to an area formed in the bottom plate.

5 . The apparatus of claim 1 , wherein the top plate is preloaded with one or more capture agents which absorb, adsorb or react with the analyte present in the sample.

6 . The apparatus of claim 5 , wherein the top plate is preloaded with at least two different capture agents in separate areas.

7 . The apparatus of claim 5 , wherein the capture agent is a nucleic acid, a peptide, a protein, an antibody, an aptamer, a bead, a particle or a cell.

8 . The apparatus of claim 1 , further comprising ink positioned in an area in a row adjacent to the plurality of channels.

9 . The apparatus of claim 1 , wherein the top plate and the bottom plate are made from a material selected from the group consisting of glass, silicon, plastics, ceramics and metal oxide.

10 . The apparatus of claim 1 , wherein the analyte is a nucleic acid, a peptide, a protein, an antibody, a cell, an organism, an allergen, a drug or its metabolites, a toxin, or an environmental pollutant.

11 . An apparatus for determining the quantity f an analyte present in a sample, the apparatus comprising: a top plate comprising a plurality of areas arranged to form a plurality of rows extending parallel to one another; and a bottom plate comprising a plurality of areas arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate; wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the areas of the top plate communicate with the areas of the bottom plate so as to forma plurality of rows; wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels; and wherein each of the plurality of channels is connected to an area formed in the bottom plate.

12 . The apparatus of claim 11 , wherein top plate is transparent.

13 . The apparatus of claim 11 , wherein at least one of the plurality of rows formed in the top plate comprises an inlet and an outlet.

14 . The apparatus of claim 11 , wherein the areas in the top plate and the areas in the bottom plate extend at a 45 degree angle relative to the axis of a row.

15 . The apparatus of claim 11 , wherein the top plate is preloaded with one or more capture agents which absorb, adsorb or react with the analyte present in the sample.

16 . The apparatus of claim 15 , wherein the top plate is preloaded with at least two different capture agents in separate areas.

17 . The apparatus of claim 15 , wherein the capture agent is a nucleic acid, a peptide, a protein, an antibody, an aptamer, a bead, a particle or a cell.

18 . The apparatus of claim 11 , further comprising ink positioned in an area in a row adjacent to the plurality of channels.

19 . The apparatus of claim 11 , wherein the top plate and the bottom plate are made from a material selected from the group consisting of glass, silicon, plastics, ceramics and metal oxide.

20 . The apparatus of claim 11 , wherein the analyte is a nucleic acid, a peptide, a protein, an antibody, a cell, an organism, an allergen, a drug or its metabolites, a toxin, or an environmental pollutant.

21 . A method for determining the quantity of an analyte present in a sample, the method comprising: providing an apparatus comprising: atop plate comprising a plurality of areas arranged to form a plurality of rows extending parallel to one another; and a bottom plate comprising a plurality of areas arranged to form a plurality of rows extending parallel to one another, and a plurality of channels extending perpendicularly to the plurality of rows of the bottom plate; wherein the top plate and the bottom plate are assembled together so that the top plate is on top of the bottom plate and the areas of the top plate communicate with the areas of the bottom plate so as to form a plurality of rows; wherein at least one of the top plate and the bottom plate is configured to slide relative to the other of the top plate and the bottom plate in order to form a plurality of columns, with each of the plurality of columns in communication with each of the plurality of channels; binding a capture agent in at least one area forming one of the plurality of rows of the top plate; introducing a sample into the at least one area so that an analyte contained in the sample is bound to the capture agent, and binding a probe to the bound analyte; and positioning a reagent in an area adjacent to the row containing the capture agent, bound analyte and bound probe; and positioning ink in an area in a row adjacent to the plurality of channels; sliding one of the top plate and the bottom plate relative to the other of the top plate and the bottom plate so as to form the plurality of columns, with each column being in communication with one of the plurality of channels; and determining the quantity of the analyte present in the sample by detecting the longitudinal position of the ink contained in the plurality of channels.

22 . The method of claim 21 , wherein the top plate is transparent.

23 . The method of claim 21 , wherein at least one of the plurality of rows formed in the top plate comprises an inlet and an outlet.

24 . The method of claim 21 , wherein the areas in the top plate and the areas in the bottom plate extend at a 45 degree angle relative to the axis of a row.

25 . The method of claim 21 , wherein each of the plurality of Channels is connected to an area formed in the bottom plate.

26 . The method of claim 21 , wherein the top plate is preloaded with one or more capture agents which absorb, adsorb or react with the analyte present in the sample.

27 . The method of claim 26 , wherein the top plate is preloaded with at least two different capture agents in separate areas.

Assignees

• Univ Chicago

Inventors

• Ismagilov Rustem F
• Du Wenbin
• Li Liang
• Shen Feng
• Nichols Kevin Paul Flood
• Chen Delai
• Kreutz Jason Eugene

Classifications

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• G01N21/78

  producing a change of colour · CPC title

• B01L2200/027

  for microfluidic devices · CPC title

• B01L7/52

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title