Cell analysis system, cell analysis program and cell analysis method

Patent metadata

Field	Value
Publication number	US-2016284081-A1
Application number	US-201415033089-A
Country	US
Kind code	A1
Filing date	Sep 18, 2014
Priority date	Nov 8, 2013
Publication date	Sep 29, 2016
Grant date	—

Abstract

[Object] To provide a cell analysis system, a cell analysis program and a cell analysis method suitable for analyzing the movement of ions or molecules across cell membranes.
[Solving Means] A cell analysis system according to the present disclosure includes a motion information extracting unit and a motion characteristics calculating unit. The motion information extracting unit extracts motion information arising from a movement of ions or molecules across a cell membrane, out of a cell image obtained from imaging a cell in time series. The motion characteristics calculating unit calculates motion characteristics of the motion information.

First claim

1 . A cell analysis system comprising:
a motion information extracting unit to extract motion information arising from a movement of ions or molecules across a cell membrane, out of a cell image obtained from imaging a cell in time series; and a motion characteristics calculating unit to calculate motion characteristics of the motion information.

2 . The cell analysis system according to claim 1 , further comprising
an image generating unit to superpose the motion characteristics and the cell image, and generate a motion characteristics displaying image.

3 . The cell analysis system according to claim 1 , further comprising
an evaluating unit to evaluate the movement of the ions or the molecules across the cell membrane, on the basis of the motion characteristics.

4 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates a motion amount as the motion characteristics, and the evaluating unit evaluates an amount of moved ions or molecules, on the basis of the motion amount.

5 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates a motion amount as the motion characteristics, and the evaluating unit evaluates an activity of an ion channel or an ionotropic receptor, on the basis of the motion amount.

6 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates as the motion characteristics a change in a movement before and after addition of an activator or an inhibitor of an ion channel or an ionotropic receptor, and the evaluating unit evaluates the presence or absence, or an amount, of the ion channel or the ionotropic receptor, on the basis of the change in the movement.

7 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates as the motion characteristics a change in a movement before and after addition of an inhibitor of an ionotropic receptor, and the evaluating unit evaluates a type or a strength of a synapse formed between cells, on the basis of the change in the movement.

8 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates a motion amount as the motion characteristics, and the evaluating unit evaluates a type or an effectiveness of a substance that acts on an ion channel or an ionotropic receptor, on the basis of the motion amount.

9 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates a motion amount as the motion characteristics, and the evaluating unit evaluates a movement, a swell, a shrink and a vibration of the cell membrane due to a movement of ions across the cell membrane or a movement of water involved, on the basis of the motion amount.

10 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates a motion direction as the motion characteristics, and the motion characteristics calculating unit evaluates a flow direction of the ions or the molecules across the cell membrane, on the basis of the motion direction.

11 . The cell analysis system according to claim 3 , wherein
the motion characteristics calculating unit calculates a duration time or a spatial distribution of a movement, and an evaluating unit evaluates a migration time of the ions or the molecules across the cell membrane or a spatial distribution of the ions or the molecules, on the basis of the duration time or the spatial distribution of the movement.

12 . The cell analysis system according to claim 1 , further comprising:
a range specifying unit to specify an extraction range in the cell image by using a luminance difference in the cell image; the motion information extracting unit extracting the motion information out of the extraction range.

13 . A cell analysis program which operates an information processing apparatus as:
a motion information extracting unit extracting motion information arising from a movement of ions or molecules across a cell membrane, out of a cell image obtained from imaging a cell in time series; and a motion characteristics calculating unit calculating motion characteristics of the motion information.

14 . A cell analysis method comprising:
extracting motion information arising from a movement of ions or molecules across a cell membrane, out of a cell image obtained from imaging a cell in time series, by a motion information extracting unit; and calculating motion characteristics of the motion information by a motion characteristics calculating unit.

Assignees

• Sony Corp

Inventors

• Nakagawa Kazuhiro
• Kishimoto Takuya
• Matsui Eriko

Classifications

• G01N33/6872

  Intracellular protein regulatory factors and their receptors, e.g. including ion channels · CPC title

• C12M41/46 Primary

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• G01N2333/705

  Assays involving receptors, cell surface antigens or cell surface determinants · CPC title

• G06T2207/30024

  Cell structures in vitro; Tissue sections in vitro · CPC title

• G06T7/0012 Primary

  Biomedical image inspection · CPC title