Sample preparation for spectroscopy analysis

Patent metadata

Field	Value
Publication number	US-2016274101-A1
Application number	US-201615168070-A
Country	US
Kind code	A1
Filing date	May 29, 2016
Priority date	Mar 3, 2010
Publication date	Sep 22, 2016
Grant date	—

Abstract

A Raman silent substrate includes a base and a biologically compatible material layer over the base. The biologically compatible material layer is Raman silent, provides a predetermined capture efficiency corresponding to a desired application, and supports biological culturing. In certain implementations, the base comprises a glass layer and an aluminum layer between the glass layer and the biologically compatible material layer.

First claim

What is claimed is:

1 . A Raman silent substrate comprising:
a base; and a biologically compatible material layer over the base; wherein the biologically compatible material layer:
is Raman silent to the extent that the Raman silent substrate does not contribute to a Raman signature collected during a spectroscopic evaluation of a biological target collected upon the Raman silent substrate;
provides a predetermined capture efficiency of the Raman signature collected during the spectroscopic evaluation; and
supports biological culturing of the biological target collected upon the substrate.

2 . The Raman silent substrate of claim 1 , wherein the base comprises:
a glass layer; and an aluminum layer between the glass layer and the biologically compatible material layer.

3 . The Raman silent substrate according to claim 2 , wherein:
a flatness and roughness of the Raman silent substrate mimics a flatness and roughness of the glass layer.

4 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a biologically compatible thin film having a thickness of 1 to 150 nanometers.

5 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a thicknesses less than a wavelength of light divided by two times a numerical aperture of collection optics of a corresponding spectroscopic system.

6 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a biologically compatible material having a Raman cross-section smaller than a predetermined Raman cross-section selected according to the spectroscopic evaluation.

7 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a biologically compatible material that is free of symmetrical molecular moieties and is further free of unsaturated chemical bonds.

8 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a biologically compatible material having a low opacity at an excitation laser wavelength of a laser source in a corresponding spectroscopic system.

9 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer has no fluorescence response.

10 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a non-selective binding material that promotes adhesion.

11 . The Raman silent substrate according to claim 10 , wherein:
the non-selective binding material comprises at least one of collagen, poly-lysine, poly-ornithine, fibronection, and laminin.

12 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a selective binding material including an antibody.

13 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a biofilm that is associated with a biological material under examination.

14 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a hydrophilic material.

15 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a hydrophobic material corresponding to a hydrophobicity of a biological material of interest.

16 . The Raman silent substrate according to claim 1 , wherein:
the base comprises a metalized portion; and the biologically compatible material layer is a material configured to prevent an exchange of metal ions between the metallized portion and biological material collected upon the substrate.

17 . The Raman silent substrate according to claim 1 , wherein:
the biologically compatible material layer comprises a biological compatible material that does not selectively bind calcium ions, sodium ions, and potassium ions.

18 . The Raman silent substrate according to claim 17 , wherein:
the biological compatible material does not significantly suppress ion diffusion in condensed phase solutions.

19 . A Raman silent substrate comprising:
a base; and a biologically compatible material layer over the base; wherein the biologically compatible material layer:
is Raman silent to the extent that the Raman silent substrate does not contribute to a Raman signature collected during a spectroscopic evaluation of a biological target collected upon the Raman silent substrate;
provides a predetermined capture efficiency of the Raman signature collected during the spectroscopic evaluation;
supports biological culturing of the biological target collected upon the substrate;
includes a thickness less than a wavelength of light divided by two times a numerical aperture of collection optics of a corresponding spectroscopic system;
is free of symmetrical molecular moieties and is further free of unsaturated chemical bonds; and
includes a hydrophobic material corresponding to a hydrophobicity of the biological target.

20 . A Raman silent substrate comprising:
a base; and a biologically compatible material layer over the base; wherein the biologically compatible material layer:
is Raman silent to the extent that the Raman silent substrate does not contribute to a Raman signature collected during a spectroscopic evaluation of a biological target collected upon the Raman silent substrate;
provides a predetermined capture efficiency of the Raman signature collected during the spectroscopic evaluation;
supports biological culturing of the biological target collected upon the substrate is free of symmetrical molecular moieties and is further free of unsaturated chemical bonds;
includes a hydrophilic material that is a non-selective binding material that promotes adhesion and is less than 150 nanometers thick.

Assignees

• Battelle Memorial Institute

Inventors

• Bartko Andrew P
• Ronningen Theodore J

Classifications

• G01N2035/00356

  Holding samples at elevated temperature (incubation) · CPC title

• G01N1/312 Primary

  for samples mounted on planar substrates · CPC title

• G01N1/2202

  involving separation of sample components during sampling · CPC title

• G01N2035/0441

  for samples · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title