Accumulation of omega-7 fatty acids in plant seeds

Patent metadata

Field	Value
Publication number	US-2016272986-A1
Application number	US-201615130788-A
Country	US
Kind code	A1
Filing date	Apr 15, 2016
Priority date	Jun 24, 2010
Publication date	Sep 22, 2016
Grant date	—

Abstract

Compositions and methods include genetically encoding and expressing a novel Δ 9 -18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel Δ 9 -18:0-ACP desaturase. In other embodiments, amino acid sequences have Δ 9 -18:0-ACP desaturase activity. Methods can involve expression of Δ 9 -18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

First claim

What is claimed is:

1 . A method for increasing the amount of unusual fatty acids in plant material, the method comprising:
transforming plant material with a nucleotide acid molecule encoding a 49 desaturase enzyme comprising a polynucleotide that is at least 60% identical to SEQ ID NO:1, such that the amount of unusual fatty acids in said plant material is increased, wherein the plant material comprises three means for increasing levels of 16:0 ACP in the plant material.

2 . The method of claim 1 , wherein the nucleic acid molecule encodes a plastidial delta-9 desaturase having at least 90% identity to SEQ ID NO:2

3 . The method of claim 1 , wherein the first means for increasing levels of 16:0-ACP in the plant material is suppression of KASII, wherein the second means for increasing levels of 16:0-ACP in the plant material is decreasing the elongation of 16:0 fatty acids in the plant material, and wherein the third means for increasing levels of 16:0-ACP in the plant material is expression of an extraplastidial desaturase.

4 . The method of claim 1 , further comprising culturing the transformed plant material to obtain a plant.

5 . The method of claim 1 , wherein the plant is selected from a genus selected from the group comprising Arabidopsis, Borago, Canola, Ricinus, Theobroma, Zea ), Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes, Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis, rapeseed, Carthamus, Glycine, Soja, Helianthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale, or other members of the Gramineae.

6 . The method of claim 1 , wherein the plant material comprises a mutant fab1 gene or a mutant fae1 gene.

7 . The method of claim 1 , wherein the plant material comprises a mutant fab1 gene and a mutant fae1 gene.

8 . The method of claim 1 , wherein the plant material is a plant or a seed.

9 . The method of claim 1 , wherein the nucleic acid molecule of claim 1 further comprises a gene regulatory element.

10 . The method of claim 1 , wherein the nucleic acid molecule of claim 9 , wherein the gene regulatory element is the phaseolin promoter or the LTP170 promoter.

11 . The method of claim 1 , wherein the 49 desaturase enzyme comprises an amino acid sequence at least 80% identical to SEQ ID NO:2.

12 . The method of claim 1 , wherein the 49 desaturase enzyme of claim 11 , wherein the amino acid at least 80% identical to SEQ ID NO:2 comprises a serine at the position analogous to position 114 in SEQ ID NO:2; an arginine at the position analogous to position117 in SEQ ID NO:2; a cysteine at the position analogous to position118 in SEQ ID NO:2, a leucine at the position analogous to position 179 in SEQ ID NO:2; or a threonine at the position analogous to position 188 in SEQ ID NO:2.

13 . The method of claim 1 , wherein the 49 desaturase enzyme of claim 11 , wherein the amino acid at least 80% identical to SEQ ID NO:2 comprises a serine at the position analogous to position 114 in SEQ ID NO:2; an arginine at the position analogous to position117 in SEQ ID NO:2; a cysteine at the position analogous to position118 in SEQ ID NO:2, a leucine at the position analogous to position 179 in SEQ ID NO:2; and a threonine at the position analogous to position 188 in SEQ ID NO:2.

14 . A plant obtained by the method of claim 4 .

15 . A plant material obtained from the plant of claim 14 .

16 . The plant material of claim 15 , wherein the plant material is a seed.

Assignees

• Brookhaven Science Ass Llc
• Dow Agrosciences Llc

Inventors

• Shanklin John
• Nguyen Tam Huu
• Walsh Terence A
• Pidkowich Mark S
• Whittle Edward J

Classifications

• C12N9/0083

  Miscellaneous (1.14.99) · CPC title

• C12Y114/19001

  Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title