Magnetic microspheres for use in fluorescence-based applications

Patent metadata

Field	Value
Publication number	US-2016266103-A1
Application number	US-201615165046-A
Country	US
Kind code	A1
Filing date	May 26, 2016
Priority date	Jan 20, 2005
Publication date	Sep 15, 2016
Grant date	—

Abstract

Microspheres, populations of microspheres, and methods for forming microspheres are provided. One microsphere configured to exhibit fluorescent and magnetic properties includes a core microsphere and a magnetic material coupled to a surface of the core microsphere. About 50% or less of the surface of the core microsphere is covered by the magnetic material. The microsphere also includes a polymer layer surrounding the magnetic material and the core microsphere. One population of microspheres configured to exhibit fluorescent and magnetic properties includes two or more subsets of microspheres. The two or more subsets of microspheres are configured to exhibit different fluorescent and/or magnetic properties. Individual microspheres in the two or more subsets are configured as described above.

First claim

1 - 24 . (canceled)

25 . A population of magnetically responsive microspheres comprising:
at least a first and a second subset of microspheres, wherein microspheres in the first and second subsets of microspheres comprise:
a core microsphere;
a magnetic material coupled to a surface of the core microsphere, wherein 50% or less of the surface of the core microsphere is covered by the magnetic material; and
a polymer layer that surrounds the magnetic material;
and further wherein the first subset of microspheres exhibits different magnetic properties as compared to the second subset of microspheres such that the microspheres in the first and second subsets of microspheres can be distinguished from each other based on their magnetic properties.

26 . The population of magnetically responsive microspheres of claim 25 , wherein the microspheres in the first and second subsets of microspheres further comprise a fluorochrome.

27 . The population of magnetically responsive microspheres of claim 26 , wherein the microspheres in the first and second subsets of microspheres further comprise two different fluorochromes.

28 . The population of magnetically responsive microspheres of claim 25 , wherein the core microsphere comprises a polymer of styrene.

29 . The population of magnetically responsive microspheres of claim 25 , wherein the core microsphere comprises a polymer of silica.

30 . The population of magnetically responsive microspheres of claim 25 , wherein the magnetic material is Fe 3 O 4 .

31 . The population of magnetically responsive microspheres of claim 25 , wherein the magnetic material comprises particles having a size of about 10 nm to about 1000 nm or aggregates of particles having a size of about 10 nm to about 1000 nm.

32 . The population of magnetically responsive microspheres of claim 25 , wherein the polymer layer comprises a polymer of styrene.

33 . The population of magnetically responsive microspheres of claim 25 , wherein the microspheres in the first and second subsets of microspheres contains greater than 2% by weight of the magnetic material.

34 . The population of magnetically responsive microspheres of claim 25 , wherein the microspheres in the first and second subsets of microspheres further comprise an additional layer of magnetic material coupled to an outer surface of the polymer layer and an additional polymer layer surrounding the additional magnetic material.

35 . The population of magnetically responsive microspheres of claim 25 , wherein the core microsphere further comprises a functional group.

36 . The population of magnetically responsive microspheres of claim 35 , wherein the functional group is selected from the group consisting of amines, thiols, carboxylic acids, hydrazine, halides, alcohols, and aldehydes.

Assignees

• Luminex Corp

Inventors

• Chandler Don J
• Bedre Jason

Classifications

• H01F1/0036

  showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity, (H01F1/153, H01F1/42 and H01F10/00 take precedence; magnetoresistive sensors G01D5/16, G01R33/06; magnetoresistive recording G11B5/39; magnetic-field-controlled resistors H10N50/10) · CPC title

• Y10T428/32

  Composite [nonstructural laminate] of inorganic material having metal-compound-containing layer and having defined magnetic layer · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• H01F1/36

  in the form of particles {(H01F1/346, H01F1/348 and H01F1/38 take precedence)} · CPC title

• Y10T428/2996

  Glass particles or spheres · CPC title