Plant growth-promoting bacteria and methods of use

Patent metadata

Field	Value
Publication number	US-2016262402-A1
Application number	US-201414775858-A
Country	US
Kind code	A1
Filing date	Mar 17, 2014
Priority date	Mar 15, 2013
Publication date	Sep 15, 2016
Grant date	—

Abstract

The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.

First claim

1 . A biologically pure bacterial culture wherein the bacteria in the bacterial culture is:
(a) Bacillus mycoides strain BT155 (NRRL No. B-50291); (b) Bacillus mycoides strain EE118 (NRRL No. B-50918); (c) Bacillus subtilis strain EE148 (NRRL No. B-50927); (d) Alcaligenes faecalis strain EE 107 (NRRL No. B-50920); (e) Bacillus mycoides strain EE141 (NRRL No. B-50916); (f) Bacillus mycoides strain BT46-3 (NRRL No. B-50922); (g) Bacillus cereus family member strain EE128 (NRRL No. B-50917); (h) Paenibacillus massiliensis strain BT23 (NRRL No. B-50923); (i) Bacillus cereus family member strain EE349 (NRRL No. B-50928); (j) Bacillus subtilis strain EE218 (NRRL No. B-50926); (k) Bacillus megaterium strain EE281 (NRRL No. B-50925) (l) salt-tolerant and thiram-resistant Paracoccus sp. strain NC35 (NRRL No. B-50948); (m) salt-tolerant and thiram resistant Bacillus mycoides strain BT155 (NRRL No. B-50949); (n) thiram-resistant Bacillus aryabhattai strain CAP53 (NRRL No. B-50946); (o) thiram-resistant Bacillus thuringiensis strain BT013A (NRRL No. B-50947); or (p) glyphosate-tolerant Bacillus aryabhattai strain CAP53 (NRRL No. B-50945).

2 .- 19 . (canceled)

20 . A method of claim 68 , wherein the bacteria in the bacterial culture are mutants of the bacteria of any of strains (a) through (1) comprising one or more mutations which retain the ability to promote plant growth.

21 . A method of claim 20 , wherein the mutant comprises a salt-tolerant mutant, a thiram-resistant mutant, a glyphosate-tolerant mutant, or a mutant with a combination of mutations to impart salt tolerance, thiram resistance and/or glyphosate tolerance.

22 .- 26 . (canceled)

27 . An inoculum for application to plants, plant seeds, or a plant growth medium, wherein the inoculum comprises an effective amount of a biologically pure bacterial culture of claim 1 and an agriculturally acceptable carrier.

28 . A method of claim 112 , wherein the inoculum comprises an effective amount of a mixture comprising at least two of the bacterial cultures.

29 .- 40 . (canceled)

41 . A method of claim 112 , wherein the inoculum further comprises an effective amount of a rhizobacteria.

42 . (canceled)

43 . A method of claim 41 , wherein the rhizobacteria comprises Bradyrhizobium genus bacteria, Rhizobium genus bacteria, or a combination thereof.

44 . A method of claim 43 , wherein the Bradyrhizobium genus bacteria comprises Bradyrhizobium japonicum.

45 . A method of claim 43 , wherein the Rhizobium genus bacteria comprises Rhizobium phaseoli, Rhizobium leguminosarum , or a combination thereof.

46 . A method of claim 112 , wherein the agriculturally acceptable carrier comprises a dispersant, a surfactant, an additive, water, a thickener, an anti-caking agent, residue breakdown, a composting formulation, a granular application, diatomaceous earth, an oil, a coloring agent, a stabilizer, a preservative, a polymer, a coating, or a combination thereof.

47 .- 52 . (canceled)

53 . A method of claim 112 , wherein the agriculturally acceptable carrier comprises vermiculite, charcoal, sugar factory carbonation press mud, rice husk, carboxymethyl cellulose, peat, perlite, fine sand, calcium carbonate, flour, alum, a starch, talc, polyvinyl pyrrolidone, or a combination thereof.

54 . A method of claim 112 , wherein the inoculum is formulated as a seed coating formulation, a liquid formulation for application to plants or to a plant growth medium, or a solid formulation for application to plants or to a plant growth medium.

55 . A method of claim 54 , wherein the seed coating formulation is an aqueous or oil-based solution for application to seeds.

56 . A method of claim 54 , wherein the seed coating formulation is a powder or granular formulation for application to seeds.

57 . A method of claim 54 , wherein the liquid formulation for application to plants or to a plant growth medium is in a concentrated formulation or a ready-to-use formulation.

58 . A method of claim 54 , wherein the solid formulation for application to plants or to a plant growth medium is a granular formulation or a powder agent.

59 . A method of claim 112 , wherein the inoculum further comprises a fertilizer, a micronutrient fertilizer material, an insecticide, a herbicide, a plant growth amendment, a fungicide, a molluscicide, an algicide, a bacterial inoculant, a fungal inoculant, or a combination thereof.

60 .- 62 . (canceled)

63 . A method of claim 59 , wherein the insecticide comprises an organophosphate, a carbamate, a pyrethroid, an acaricide, an alkyl phthalate, boric acid, a borate, a fluoride, sulfur, a haloaromatic substituted urea, a hydrocarbon ester, a biologically-based insecticide, or a combination thereof.

64 . (canceled)

65 . A method of claim 59 , wherein the fungicide comprises a substituted benzene, a thiocarbamate, an ethylene bis dithiocarbamate, a thiophthalidamide, a copper compound, an organomercury compound, an organotin compound, a cadmium compound, anilazine, benomyl, cyclohexamide, dodine, etridiazole, iprodione, metlaxyl, thiamimefon, triforine, or a combination thereof.

66 . (canceled)

67 . A method of claim 59 , wherein the bacterial inoculant comprises a bacterial inoculant of the genus Rhizobium , a bacterial inoculant of the genus Bradyrhizobium , a bacterial inoculant of the genus Mesorhizobium , a bacterial inoculant of the genus Azorhizobium , a bacterial inoculant of the genus Allorhizobium , a bacterial inoculant of the genus Sinorhizobium , a bacterial inoculant of the genus Kluyvera , a bacterial inoculant of the genus Azotobacter , a bacterial inoculant of the genus Pseudomonas , a bacterial inoculant of the genus Azospirillium , a bacterial inoculant of the genus Bacillus , a bacterial inoculant of the genus Streptomyces , a bacterial inoculant of the genus Paenibacillus , a bacterial inoculant of the genus Paracoccus , a bacterial inoculant of the genus Enterobacter , a bacterial inoculant of the genus Alcaligenes , a bacterial inoculant of the genus Mycobacterium , a bacterial inoculant of the genus Trichoderma , a bacterial inoculant of the genus Gliocladium , a bacterial inoculant of the genus Glomus , a bacterial inoculant of the genus Klebsiella , or a combination thereof.

68 . A method for stimulating plant growth comprising applying a bacterial culture to a plant, plant seed, or plant growth medium, wherein the bacteria in the bacterial culture comprises:
(a) Bacillus mycoides strain BT155 (NRRL No. B-50291); (b) Bacillus mycoides strain EE118 (NRRL No. B-50918); (c) Bacillus subtilis strain EE148 (NRRL No. B-50927); (d) Alcaligenes faecalis strain EE107 (NRRL No. B-50920); (e) Bacillus mycoides strain EE141 (NRRL No. B-50916); (f) Bacillus mycoides strain BT46-3 (NRRL No. B-50922); (g) Bacillus cereus family member strain EE128 (NRRL No. B-50917); (h) Bacillus thuringiensis strain BT013A (NRRL No. B-50924); (i) Paenibacillus massiliensis strain BT23 (NRRL No. B-50923); (j) Bacillus cereus family member strain EE349 (NRRL No. B-50928); (k) Bacillus subtilis strain EE218 (NRRL No. B-50926); of (l) Bacillus megaterium strain EE281 (NRRL No. B-50925); (m) salt-tolerant and thiram-resistant Paracoccus sp. strain NC35 (NRRL No. B-5

Assignees

• Spogen Biotech Inc

Inventors

• Thompson Brian
• Thompson Katie
• Angle Brittany

Classifications

• C12R1/125

  Chemistry & Metallurgy · mapped topic

• C12R1/085

  Chemistry & Metallurgy · mapped topic

• C12R1/075

  Chemistry & Metallurgy · mapped topic

• A01N63/02 Primary

  Human Necessities · mapped topic

• C12R1/05

  Chemistry & Metallurgy · mapped topic