Milling Process

Patent metadata

Field	Value
Publication number	US-2016257981-A1
Application number	US-201415033751-A
Country	US
Kind code	A1
Filing date	Nov 26, 2014
Priority date	Nov 26, 2013
Publication date	Sep 8, 2016
Grant date	—

Abstract

Provided are processes for treating crop kernels which comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of a polypeptide having protease activity.

First claim

1 . A process for treating crop kernels, comprising the steps of:
a) soaking kernels to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of a polypeptide having protease activity, wherein step c) is performed before, simultaneously with or after step b), and said polypeptide is a serine protease of the peptidase family S53 or selected from the group consisting of: (a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 5, SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the mature polypeptide coding sequence of SEQ ID NO: 3, (iii) the full-length complementary strand of (i) or (ii); (c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3; (d) a variant of the polypeptide of SEQ ID NO: 5, SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (several) positions; and (e) a fragment of a polypeptide of (a), (b), (c) or (d) having protease activity.

2 . The process of claim 1 , wherein the said polypeptide is a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO: 5, SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

3 . The process of claim 1 , wherein the said polypeptide is a polypeptide encoded by a polynucleotide having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3.

4 . The process of claim 1 , wherein the said polypeptide having protease activity comprising or consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5 or SEQ ID NO: 6, or SEQ ID NO: 24 or SEQ ID NO: 25 disclosed in WO 2014/037438.

5 . The process of claim 1 , wherein the polypeptide having protease activity is a serine protease, such as a serine protease of family S53.

6 . The process of claim 1 , wherein the polypeptide having protease activity is from the genus Meripilus , genus Dichomitus , or genus Trametes.

7 . The process of claim 1 , further comprising treating the soaked kernels in the presence of a beta-xylosidase.

8 . The process of claim 1 , further comprising treating the soaked kernels in the presence of a cellulase and/or a hemicelluase.

9 . The process of claim 1 , further comprising treating the soaked kernels in the presence of a xylanase.

10 . The process of claim 1 , further comprising treating the soaked kernels in the presence of a cellulolytic composition comprising: 1) a cellulase or a hemicellulase, and 2) a GH61 polypeptide.

11 . The process of claim 1 , further comprising treating the soaked kernels in the presence of an acetylxylan esterase.

12 . The process of claim 1 , further comprising treating the soaked kernels in the presence of an enzyme selected from the group consisting of an endoglucanase, a xylanase, a cellobiohydrolase I, a cellobiohydrolase II, a GH61 polypeptide, or a combination thereof.

13 . The process of claim 1 , wherein said polypeptide is present in an amount of preferably 0.0005 to 1.5 mg enzyme protein per g DS kernels, preferably 0.001 to 1 mg enzyme protein per g DS kernels, preferably 0.01 to 0.5 mg enzyme protein per g DS kernels, preferably 0.025 to 0.25 mg enzyme protein per g DS kernels.

14 . The process of claim 1 , wherein the crop kernels are from corn (maize), rice, barley, sorghum bean, or fruit hulls, or wheat.

15 . An enzyme composition comprising a polypeptide having protease activity, wherein said polypeptide is selected from the group consisting of:
(a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 5, SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the mature polypeptide coding sequence of SEQ ID NO: 3, (iii) the full-length complementary strand of (i) or (ii); (c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or SEQ ID NO: 3; (d) a variant of the polypeptide of SEQ ID NO: 5, SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (several) positions; and (e) a fragment of a polypeptide of (a), (b), (c) or (d) having protease activity.

16 . The composition of claim 15 , wherein the polypeptide having protease activity comprising or consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO:5 or SEQ ID NO: 6.

17 . The composition of claim 15 , wherein the polypeptide having protease activity is a serine protease.

18 . The composition of claim 1 , wherein the said polypeptide having protease activity is from the genus Meripilus.

19 . The composition of claim 1 , further comprising of an enzyme selected from the group consisting of a beta-xylosidase, a cellulase, a hemicelluase, a xylanase, an endoglucanase, a GH61 polypeptide, or a combination thereof.

20 . The composition of claim 1 , further comprising a beta-xylosidase, a xylanase and an endoglucanase.

21 . (canceled)

Assignees

• Novozymes As

Inventors

• Long Zhen
• Xu Wanghui
• Han Wang
• Mclaughlin Scott R
• Deinhammer Randall
• Saunders Paria
• Vidal Jr Bernardo
• Shen Xinyu
• Akerman Michael John
• Gibbons Tom

Classifications

• C12P19/04 Primary

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C08B30/042

  from cereals or grains · CPC title

• C08B30/02

  Preparatory treatment, e.g. crushing of raw materials {or steeping process (machines for preliminary washing A23N)} · CPC title

• C12N9/6408

  Serine endopeptidases (3.4.21) · CPC title

• C08B30/044

  from corn or maize · CPC title