Transformed synechococcus elongatus having capability of producing acetone from carbon dioxide

Patent metadata

Field	Value
Publication number	US-2016257966-A1
Application number	US-201615057551-A
Country	US
Kind code	A1
Filing date	Mar 1, 2016
Priority date	Mar 4, 2015
Publication date	Sep 8, 2016
Grant date	—

Abstract

Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

First claim

What is claimed is:

1 . A Synechococcus elongatus strain having an acetone selectivity, defined as the molar ratio of acetone in the total product produced by the strain, of 0.8 or greater under a condition of 30° C. and 5% carbon dioxide.

2 . The strain according to claim 1 , wherein the strain comprises:
one or more selected from a group consisting of an acetyl-CoA transferase gene and an acetyl-CoA synthase gene; an acetoacetyl-CoA transferase gene; and an acetoacetate decarboxylase gene.

3 . The strain according to claim 2 , wherein the acetyl-CoA transferase gene comprises a sequence of SEQ ID NO 1, the acetyl-CoA synthase gene comprises a sequence of SEQ ID NO 2, the acetoacetyl-CoA transferase gene comprises a sequence of sequence of SEQ ID NO 3 or SEQ ID NO 4, and the acetoacetate decarboxylase gene comprises a sequence of sequence of SEQ ID NO 5.

4 . The strain according to claim 1 , wherein the strain is one transformed with a vector comprising: a gene comprising a sequence derived from an atoB gene or an nphT7 gene; a gene comprising a sequence derived from an atoDA gene or a cftAB gene; and a gene comprising a sequence derived from an adc gene.

5 . The strain according to claim 4 , wherein the strain is Synechococcus elongatus PCC7942 transformed with the vector.

6 . The strain according to claim 4 , wherein the vector further comprises:
a pUC replication origin as a replication origin; neutral sites located upstream and downstream of the replication origin; a spectinomycin resistance gene as a selection marker; a repressor selected from a group consisting of a lac I repressor, a tetR repressor and an AraC repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter; and a BglII site, a BamHI site, an EcoRI site and an XhoI site as restriction enzyme sites.

7 . The strain according to claim 6 , wherein the gene comprising a sequence derived from the atoB gene, the gene comprising a sequence derived from the nphT7 gene, the gene comprising a sequence derived from the atoDA gene, the gene comprising a sequence derived from the cftAB gene and the gene comprising a sequence derived from the adc gene are located between the BglII site and the BamHI site.

8 . The strain according to claim 4 , wherein the vector comprises a sequence from SEQ ID NOS 6-9.

9 . The strain according to claim 3 , wherein the strain is a Synechococcus elongatus strain of accession number KCTC12758BP, KCTC12759BP, KCTC12760BP or KCTC12761BP.

10 . The strain according to claim 1 , wherein the strain absorbs and fixes carbon dioxide.

Assignees

• Korea Inst Sci & Tech

Inventors

• Woo Han Min
• Um Youngsoon
• Chwa Jun Won
• Gong Gyeongtaek

Classifications

• C12N9/88 Primary

  Lyases (4.) · CPC title

• C12N15/82 Primary

  for plant cells {, e.g. plant artificial chromosomes (PACs)} · CPC title

• C12Y401/01004

  Acetoacetate decarboxylase (4.1.1.4) · CPC title

• C12N9/13

  transferring sulfur containing groups (2.8) · CPC title

• C12Y208/03009

  Butyrate--acetoacetate CoA-transferase (2.8.3.9) · CPC title