Sulfated Cellulose Hydrate Membrane, Method for Producing Same, and Use of the Membrane as an Adsorption Membrane for a Virus Purification Process

Patent metadata

Field	Value
Publication number	US-2016257941-A1
Application number	US-201415029203-A
Country	US
Kind code	A1
Filing date	Sep 11, 2014
Priority date	Oct 14, 2013
Publication date	Sep 8, 2016
Grant date	—

Abstract

The present invention relates to a sulfated cellulose hydrate membrane, a method for the preparation thereof and the use of the membrane as adsorption membrane for the purification of viruses.

First claim

1 . A sulfated cellulose hydrate membrane comprising a crosslinked cellulose hydrate matrix with pores which extend from one main surface to another main surface of the membrane, wherein the cellulose hydrate membrane has sulfate ligands on its inner and outer surfaces for adsorptive substance separation.

2 . The sulfated cellulose membrane as claimed in claim 1 , wherein the degree of sulfation of the cellulose hydrate matrix is 10% by weight or more.

3 . The sulfated cellulose hydrate membrane a claimed in claim 1 , wherein the mean pore size of the membrane is between 0.5 and 5.0 μm.

4 . The sulfated cellulose hydrate membrane as claimed in claim 13 , wherein the degree of crosslinking of the cellulose hydrate matrix is 0.05 to 0.5.

5 . A method for preparing a sulfated cellulose hydrate membrane as claimed in claim 1 , comprising the steps of:
providing a cellulose membrane with a pore size of 0.1 to 20 μm; crosslinking the cellulose hydrate matrix using a crosslinker having at least two functional groups in the molecule which react with the hydroxyl groups of the cellulose hydrate matrix; and sulfating the crosslinked cellulose hydrate matrix.

6 . The method as claimed in claim 5 , wherein the crosslinker is selected from the group consisting of diepoxide compounds, diisocyanates, epichlorohydrin, epibromohydrin, dimethylurea, dimethylethyleneurea, dimethylchlorsilan, bis(2-hydroxyethylsulfone), divinylsulfone, alkylene dihalides, hydroxyalkylene dihalides and diglycidyl ethers or a mixture thereof.

7 . The method as claimed in claim 5 , wherein 1,4-butanediol diglycidyl ether or ethylene glycol diglycidyl ether is used as crosslinker.

8 . The method as claimed in claim 5 , wherein the concentration of the crosslinker in the crosslinking solution of 10 to 30% by weights.

9 . The method as claimed in claim 5 , wherein the sulfation is effected by reacting the crosslinked cellulose hydrate matrix having a Lewis base-SO 3 complex.

10 . The method as claimed in claim 5 , wherein the sulfation is effected by reaction with a SO 3 -pyridine complex.

11 . The method as claimed in claim 10 , wherein the concentration of SO 3 -pyridine-complex in the sulfation solution is 1 to 40% by weight.

12 . The method as claimed in claim 5 , wherein the sulfation is effected at a temperature of 20 to 90° C.

13 . A method of purifying viruses or virus fragments comprising:
preparing a sulfated cellulose hydrate membrane as claimed in claim 1 ; and contacting the membrane with a solution containing the viruses or virus fragments.

14 . The method of claim 13 wherein the viruses have a molecular mass of greater than 10 7 Da.

15 . The method of claim 13 wherein the viruses are influenza viruses.

Assignees

• Sartorius Stedim Biotech Gmbh

Inventors

• Villain Louis
• Hörl Hans-Heinrich
• Brumm Christian

Classifications

• C12N2760/16151

  Methods of production or purification of viral material · CPC title

• B01D2325/24

  Mechanical properties, e.g. strength · CPC title

• B01D71/20

  Esters of inorganic acids, e.g. cellulose nitrate · CPC title

• B01D69/02 Primary

  characterised by their properties · CPC title

• B01D2323/30

  Cross-linking · CPC title