Chemiluminescence Measurement Device and Method for Same

Patent metadata

Field	Value
Publication number	US-2016245756-A1
Application number	US-201415027297-A
Country	US
Kind code	A1
Filing date	Oct 7, 2014
Priority date	Oct 7, 2013
Publication date	Aug 25, 2016
Grant date	—

Abstract

The present invention relates to a device and method for measuring chemiluminescence, and an object is to provide a compact and reliable device and method for measuring chemiluminescence.
The device includes a vessel group, a nozzle head having a gas suctioning and discharging mechanism, one or more nozzles, which are in communication with the suctioning and discharging mechanism, and to a front end of which a dispensing tip is attachable, and a magnetic force means capable of causing a magnetic field inside the attached dispensing tip, a nozzle moving mechanism, a linkage part provided to the nozzle head, linkable to the reaction vessel and capable of forming a confined space shielded from external light, a linkage part moving mechanism capable of moving the linkage part, a photometer, a shutter, and a reagent injection flow channel capable of injecting a reagent for chemiluminescence.

First claim

1 . A device for measuring chemiluminescence, comprising:
a vessel group at least having one or more liquid reception parts and one or more reaction vessels; a nozzle head having a suctioning and discharging mechanism for carrying out suctioning and discharging of gas, one or more nozzles, which are in communication with the suctioning and discharging mechanism, and to a front end of which a dispensing tip is attachable, and a magnetic force means capable of causing a magnetic field inside the attached dispensing tip; a nozzle moving mechanism capable of moving the nozzle relative to the vessel group; a linkage part provided to the nozzle head, linkable to the reaction vessel through an opening thereof and capable of forming a confined space shielded from external light by means of linkage with the reaction vessel; a linkage part moving mechanism capable of moving the linkage part relative to the reaction vessel; a photometer; a reagent injection flow channel capable of injecting a reagent for chemiluminescence for producing chemiluminescence, which is provided to the linkage part, and a front end of which is located in the reaction vessel by means of the linkage of the linkage part to the reaction vessel; and a shutter for bringing into a light guiding state between inside the reaction vessel and the photometer in relation to the linkage of the linkage part to the reaction vessel and bringing into a light shielding state between inside the reaction vessel and the photometer after the linkage, before release of the linkage, and after a predetermined measuring time depending on one or more factors selected from the group consisting of sensitivity of the photometer, kind of a substance for chemiluminescence, lifetime of the chemiluminescence and amount of the substance for chemiluminescence passes from injection of the reagent for chemiluminescence.

2 . The device for measuring chemiluminescence according to claim 1 , further comprising:
one or more light guiding part capable of guiding light between the reaction vessel and the photometer, wherein the shutter switches the light guiding part between a light guiding state and a light shielding state.

3 . The device for measuring chemiluminescence according to claim 2 , further comprising a connection end arrangement body in which two or more connection ends are arranged along a predetermined course on a predetermined arrangement surface, and a light reception switching mechanism for sequentially guiding light between inside the reaction vessels and the photometer by relatively moving the photometer along the predetermined course, wherein the linkage part moving mechanism is capable of relatively moving the linkage part so as to be sequentially linkable to the two or more reaction vessels through the openings, and front ends of the two or more guiding parts are provided so as to be directly or indirectly in proximity to or in contact with the reaction vessels, and rear ends thereof are provided to the corresponding connection ends.

4 . The device for measuring chemiluminescence according to claim 1 , comprising a reagent injecting mechanism, which comprises the reagent injection flow channel, for injecting the reagent for chemiluminescence through the reagent injection flow channel when or after the shutter brings into a light guiding state to optically connect the photometer to inside the reaction vessel.

5 . The device for measuring chemiluminescence according to claim 3 , wherein the light reception switching mechanism optically connects, depending on the linkage of the linkage part to each of the reaction vessels, each of the connection ends to the photometer correspondingly, and depending on the release of the linkage, releases the connection.

6 . A method for measuring chemiluminescence, comprising the steps of:
attaching a dispensing tip to a nozzle of a nozzle head, dispensing through the attached dispensing tip a sample solution, a solution of a substance for chemiluminescence or a solution containing magnetic particles capable of having a substance of interest bound, which are each received in a corresponding liquid reception part, labeling the substance of interest with the substance for chemiluminescence, causing a magnetic field in the dispensing tip, carrying out suctioning and discharging to separate the magnetic particles to which the substance of interest binds, and dispensing into a reaction vessel a solution containing the labeled substance of interest dissociated from the separated magnetic particles; moving a linkage part relative to the one reaction vessel to link through an opening to the linkage part to form a confined space shielded from external light; switching from a light shielding state to a light guiding state between inside the reaction vessel and a photometer in relation to the linkage of the linkage part to the reaction vessel; measuring an optical state in the reaction vessel by the photometer after the linkage, and before release of the linage, for a predetermined measuring time depending on one or more factors selected from the group consisting of sensitivity of the photometer, kind of the substance for chemiluminescence, lifetime of the chemiluminescence and amount of the substance for chemiluminescence from injection of the reagent for chemiluminescence for producing chemiluminescence; and switching from the light guiding state to a light shielding state between inside the reaction vessel and the photometer after the predetermined measuring time passes and before the release of the linkage of the linkage part to the reaction vessel.

7 . The method for measuring chemiluminescence according to claim 6 , further comprising the step of:
injecting a reagent for chemiluminescence for producing chemiluminescence into the reaction vessel through a reagent injection flow channel, which is provided to the linkage part, and a front end of which is located inside the reaction vessel by means of the linkage of the linkage part to the reaction vessel, when or after switching from a light shielding state to a light guiding state between inside the reaction vessel and the photometer, wherein the measuring step measures an optical state in the reaction vessel by the photometer for a predetermined measuring time after injection.

8 . The method for measuring chemiluminescence according to claim 6 , wherein
the switching step from a light shielding state to a light guiding state switches from a light shielding state to a light guiding state between the reaction vessel and a connection end corresponding to the reaction vessel arranged along a predetermined course on a predetermined arrangement surface interlocking with the linkage of the linkage part to the reaction vessel, and carrying out an optical connection, interlocks with the linkage of the linkage part to the reaction vessel, between the corresponding connection end and the photometer, and the switching step from a light guiding state to a light shielding state switches from the light guiding state to a light shielding state between the reaction vessel and the corresponding connection end interlocking with the release of the linkage of the linkage part to the reaction vessel, or releases the optical connection of the connection end to the photometer.

9 . The method for measuring chemiluminescence according to claim 7 , wherein
the switching step from a light shielding state to a light guiding state switches from a light shielding state to a light guiding state between the reaction vessel and a connection end corresponding to the reaction vessel arranged along a predetermined course on a predetermined arrangement surface interlocking with the linkage of the linkage part to the reaction vessel, and carrying

Assignees

• Universal Bio Research Co Ltd

Inventors

• Tajima Hideji

Classifications

• G01N21/76 Primary

  Chemiluminescence; Bioluminescence · CPC title

• G01N35/0098

  involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C) · CPC title

• G01N35/1002

  Reagent dispensers · CPC title

• C12Q1/6816

  characterised by the detection means (C12Q1/6804 takes precedence) · CPC title

• Y10T436/119163

  with aspirator of claimed structure · CPC title