Essential Oils Or Volatile Organics Thereof Electrospun In Chitosan Nanofiber Mats

Patent metadata

Field	Value
Publication number	US-2016243271-A1
Application number	US-201415027359-A
Country	US
Kind code	A1
Filing date	Oct 8, 2014
Priority date	Oct 8, 2013
Publication date	Aug 25, 2016
Grant date	—

Abstract

Nanofiber mats comprising chitosan, chitosan-essential oils and, in particular, chitosan-cinnamaldehyde (chitosan-CA) are described, including methods of making the nanofiber mats and methods of using same, for example, for inactivating bacteria such as E. coli and P. aeruginosa , and as therapeutic wound dressings to treat nosocomial pseudomonas infections.

First claim

What is claimed is:

1 . A method of preparing a nanofiber mat, the mat comprising an essential oil or a volatile organic thereof, the method comprising electrospinning a mixture of chitosan, poly(ethylene oxide), an acid, and an essential oil or a volatile organic thereof, wherein the mixture of has a pH of approximately 4, thereby preparing a nanofiber mat comprising an essential oil.

2 . The method of claim 1 , wherein the ratio of chitosan to poly(ethylene oxide) is approximately 1:1.

3 . The method of claim 1 , further comprising the step of mixing the chitosan and poly(ethylene oxide) for approximately 24 hours.

4 . The method of claim 1 , wherein the acid is acetic acid.

5 . The method of claim 1 , wherein the applied voltage for the electrospinning is approximately 25 kV.

6 . The method of claim 1 , wherein the molecular weight of the chitosan is between about 460,000 Da and about 1,000,000 Da.

7 . The method of claim 1 wherein the essential oil is cinnamaldehyde.

8 . The method of claim 7 , wherein the amount of cinnamaldehyde is approximately 0.5 v/v %.

9 . The method of claim 7 , wherein the amount of cinnamaldehyde is approximately 5.0 v/v %.

10 . The method of claim 1 wherein the essential oil or volatile organic thereof is hydrocinnamic alcohol.

11 . A nanofiber mat prepared by the method of claim 1 .

12 . The nanofiber mat of claim 11 , wherein the ratio of chitosan to poly(ethylene oxide) is approximately 1:1.

13 . The nanofiber mat of claim 11 , further comprising the step of mixing the chitosan and poly(ethylene oxide) for approximately 24 hours.

14 . The nanofiber mat of claim 11 , wherein the acid is acetic acid.

15 . The nanofiber mat of claim 11 , wherein the essential oil is cinnamaldehyde and the amount of cinnamaldehyde is approximately 0.5 v/v %.

16 . The nanofiber mat of claim 11 , wherein the essential oil is cinnamaldehyde and the amount of cinnamaldehyde is approximately 5.0 v/v %.

17 . The nanofiber mat of claim 11 , wherein the applied voltage for the electrospinning is approximately 25 kV.

18 . The nanofiber mat of claim 11 , wherein the nanofibers have a diameter of between approximately 38 nm and 55 nm.

19 . A method of inactivating microbes comprising contacting a nanofiber mat prepared as in claim 1 with the microbe under time and conditions sufficient for the microbe to be partially, or completely, inactivated.

20 . The method of claim 19 wherein the microbe is E. coli or P. aeruginosa.

21 . The method of claim 19 wherein the bacteria has formed a biofilm and the time and conditions the nanofiber mat is contacted with the microbial biofilm is sufficient for the dispersion of the biofilm.

Assignees

• Univ Massachusetts

Inventors

• Schiffman Jessica D
• Rieger Katrina A

Classifications

• A61L15/225 Primary

  Mixtures of macromolecular compounds · CPC title

• A61L2300/404

  Biocides, antimicrobial agents, antiseptic agents · CPC title

• A61L15/425

  Porous materials, e.g. foams or sponges · CPC title

• A61L15/26

  Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof {(A61L15/225 takes precedence)} · CPC title

• A61L15/20 Primary

  containing organic materials · CPC title