Microfluidic Assay Devices And Methods For Making And Using The Same

Patent metadata

Field	Value
Publication number	US-2016238595-A1
Application number	US-201415030232-A
Country	US
Kind code	A1
Filing date	Oct 21, 2014
Priority date	Oct 22, 2013
Publication date	Aug 18, 2016
Grant date	—

Abstract

Microfluidic devices for determining whether an analyte is present in a sample are provided. The microfluidic devices include a polymeric medium that includes a first analyte detection domain having a first covalently bound capture member that specifically binds to a first analyte, and a second analyte detection domain having a second covalently bound capture member that specifically binds to a second analyte. Also provided are methods of using the subject microfluidic device, systems and kits that use the subject microfluidic devices, as well as methods of producing the same.

First claim

1 . A microfluidic device comprising:
a polymeric medium comprising:
a first analyte detection domain comprising a first covalently bound capture member that specifically binds to a first analyte; and
a second analyte detection domain comprising a second covalently bound capture member that specifically binds to a second analyte.

2 . The microfluidic device of claim 1 , wherein the first and second covalently bound capture members are different.

3 . The microfluidic device of claim 1 , wherein the first and second covalently bound capture members are the same, and the microfluidic device further comprises a spacer domain between the first and second analyte detection domains.

4 . The microfluidic device of claim 1 , wherein the polymeric medium comprises a polyacrylamide gel.

5 . The microfluidic device of claim 1 , wherein the first covalently bound capture member is covalently bound to the polymeric medium through a linker group.

6 . The microfluidic device of claim 1 , wherein the second covalently bound capture member is covalently bound to the polymeric medium through a linker group.

7 . The microfluidic device of claim 5 , wherein the linker group comprises a benzophenone functional group.

8 . The microfluidic device of claim 5 , wherein the linker group comprises N-(3-[(4-benzoylphenyl)formamido]propyl) methacrylamide or 3-benzoyl-N-[3-(2-methyl-acryloylamino)-propyl]-benzamide.

9 . The microfluidic device of claim 1 , wherein the first capture member comprises an antigen.

10 . The microfluidic device of claim 1 , wherein the second capture member comprises an antigen.

11 . The microfluidic device of claim 1 , wherein the microfluidic device comprises two or more polymeric media, each of which comprises:
(a) a first analyte detection domain comprising a first covalently bound capture member that specifically binds to a first analyte; and (b) a second analyte detection domain comprising a second covalently bound capture member that specifically binds to a second analyte.

12 . A method of determining whether an analyte is present in a sample, the method comprising:
(a) introducing a sample into a microfluidic device comprising a polymeric medium, wherein the polymeric medium comprises:
(i) a first analyte detection domain comprising a first covalently bound capture member that specifically binds to a first analyte; and
(ii) a second analyte detection domain comprising a second covalently bound capture member that specifically binds to a second analyte;
(b) applying a directional electric field to the polymeric medium in a manner sufficient to move components in the sample through the polymeric medium; and (c) obtaining a signal from one or more of the first and second analyte detection domains to determine whether the analyte is present in the sample.

13 . The method of claim 12 , wherein the first capture member comprises a first antigen and the first analyte comprises a first specific binding member that specifically binds to the first antigen.

14 . The method of claim 12 , wherein the second capture member comprises a second antigen and the second analyte comprises a second specific binding member that specifically binds to the second antigen.

15 . The method of claim 13 , wherein the first specific binding member comprises a fluorescent label.

16 . The method of claim 14 , wherein the second specific binding member comprises a fluorescent label.

17 . The method of claim 12 , further comprising introducing a label into the microfluidic device after introducing the sample into the microfluidic device.

18 . The method of claim 17 , wherein the label comprises a secondary specific binding member that specifically binds to the first analyte.

19 . The method of claim 17 , wherein the label comprises a secondary specific binding member that specifically binds to the second analyte.

20 . The method of claim 17 , wherein the label comprises a fluorescent moiety.

21 . The method of claim 12 , wherein the sample comprises blood or a blood product.

22 . A system for determining whether an analyte is present in a sample, the system comprising:
(a) a microfluidic device according to claim 1 ; and (b) a detector.

23 . The system according to claim 22 , further comprising one or more microfluidic components configured to direct a fluid through the microfluidic device.

24 . A kit comprising:
(a) a microfluidic device according to claim 1 ; (b) a packaging configured to contain the microfluidic device.

25 . A method of producing a microfluidic assay device, the method comprising:
producing a polymeric medium comprising functional groups that covalently bond to a capture member upon application of an applied stimulus; introducing into the polymeric medium a first capture member that specifically binds to a first analyte; exposing a first region of the polymeric medium to the applied stimulus to produce a first analyte detection domain that comprises the first capture member covalently bound to the polymeric medium; introducing into the polymeric medium a second capture member that specifically binds to a second analyte; and exposing a second region of the polymeric medium to the applied stimulus to produce a second analyte detection domain that comprises the second capture member covalently bound to the polymeric medium, to produce the microfluidic assay device.

Assignees

• Univ California

Inventors

• Herr Amy E
• Lin Robert

Classifications

• B01L2300/0627

  Sensor or part of a sensor is integrated · CPC title

• G01N33/545

  Synthetic resin · CPC title

• G01N33/561

  Immunoelectrophoresis · CPC title

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title