Probe set for isothermal one-pot reaction for detecting strains with biologically active biosynthetic pathway and uses thereof
US-2024376553-A1 · Nov 14, 2024 · US
US2016237478A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016237478-A1 |
| Application number | US-201415027862-A |
| Country | US |
| Kind code | A1 |
| Filing date | Oct 7, 2014 |
| Priority date | Oct 7, 2013 |
| Publication date | Aug 18, 2016 |
| Grant date | — |
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Disclosed herein are method, materials and kits for detection of B. Burgdorferi infection or determining stage of Lyme disease in a subject. Exemlified is a method of diagnosing an infection in a subject, the method involving exposing a biological sample from the subject to a capture substrate under conditions for an infection marker in said biological sample to associate with the capture substrate to form a capture complex; associating said capture complex with a marker complex, said marker complex comprising an oligonucleotide; and amplifying said oligonucleotide of marker complex associated with said capture complex to produce an amplification signal; wherein an amplification signal above a predetermined signal threshold indicates that said subject is infected.
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What is claimed is: 1 . A method of diagnosing an infection in a subject, the method comprising: exposing a biological sample from the subject to a capture substrate under conditions for an infection marker in said biological sample to associate with the capture substrate to form a capture complex; associating said capture complex with a marker complex, said marker complex comprising an oligonucleotide; and amplifying said oligonucleotide of marker complex associated with sa…
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