Method of producing retinal pigment epithelial cell

Patent metadata

Field	Value
Publication number	US-2016237403-A1
Application number	US-201415028076-A
Country	US
Kind code	A1
Filing date	Oct 9, 2014
Priority date	Oct 9, 2013
Publication date	Aug 18, 2016
Grant date	—

Abstract

Provided are a production method of retinal pigment epithelial (RPE) cells that improves differentiation induction efficiency of pluripotent stem cells into RPE cells, and can provide highly pure RPE cells by a simple and easy operation in a short period, a culture method of RPE cells that can stably grow and culture a cell, a toxicity/efficacy evaluation method using RPE cells useful for transplantation therapy, and a therapeutic drug for a retinal disease. The invention relates to a production method of RPE cells, comprising adhesion culture of human pluripotent stem cells using a culture substrate coated with a laminin-E8 fragment, a culture method of RPE cells, comprising adhesion culture of RPE cells using a culture substrate coated with a laminin-E8 fragment, a toxicity or efficacy evaluation method using RPE cells obtained by producing or culturing by the method, and a therapeutic drug for a retinal disease, containing the RPE cells.

First claim

1 . A production method of retinal pigment epithelial cells, comprising a step of adhesion culture of human pluripotent stem cells on a culture substrate coated with a laminin-E8 fragment.

2 . The method according to claim 1 , wherein the laminin-E8 fragment is laminin-511E8 fragment.

3 . The method according to claim 1 , wherein the step of adhesion culture is performed in the presence of a differentiation-inducing factor.

4 . The method according to claim 1 , wherein the human pluripotent stem cells are human iPS cells.

5 . A retinal pigment epithelial cell produced by the method according to claim 1 .

6 . A retinal pigment epithelial cell sheet produced by the method according to claim 1 .

7 . An amplification method of retinal pigment epithelial cells, comprising a step of adhesion culture of retinal pigment epithelial cells on a culture substrate coated with a laminin-E8 fragment.

8 . A purification method of retinal pigment epithelial cells, comprising a step of adhesion culture of retinal pigment epithelial cells on a culture substrate coated with a laminin-E8 fragment.

9 . A therapeutic drug for a retinal disease, comprising retinal pigment epithelial cells produced by the method according to claim 1 .

10 . A therapeutic drug for a retinal disease, comprising retinal pigment epithelial cells cultured by the method according to claim 7 .

11 . A method of treating a retinal disease in a human subject, comprising transplanting retinal pigment epithelial cells produced by the method according to claim 1 to the subject, thereby treating the retinal disease.

12 . A method of treating a retinal disease in a human subject, comprising transplanting retinal pigment epithelial cells cultured by the method according to claim 7 to the subject, thereby treating the retinal disease.

13 . A method of treating a retinal disease in a human subject, comprising transplanting a retinal pigment epithelial cell sheet produced by the method according to claim 1 to the subject, thereby treating the retinal disease.

Assignees

• Healios Kk
• Riken
• Univ Osaka

Inventors

• Sawada Masanori
• Takahashi Masayo
• Sekiguchi Kiyotoshi

Classifications

• A61P27/02

  Ophthalmic agents · CPC title

• C12N2506/45

  from artificially induced pluripotent stem cells · CPC title

• C12N5/0621 Primary

  Eye cells, e.g. cornea, iris pigmented cells (photoreceptors C12N5/062) · CPC title

• A61K35/30

  Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue · CPC title

• C12N2533/52

  Fibronectin; Laminin · CPC title