Method for preparing a chitosan-based matrix comprising a fiber reinforcement member

Patent metadata

Field	Value
Publication number	US-2016237228-A1
Application number	US-201615003975-A
Country	US
Kind code	A1
Filing date	Jan 22, 2016
Priority date	Feb 17, 2015
Publication date	Aug 18, 2016
Grant date	—

Abstract

The present invention relates to a method for preparing a chitosan-based matrix ( 4 ) comprising the following steps:
a) two separate chitosan porous layers ( 1, 2 ) obtained from lyophilisation of layers of one or more solutions of chitosan are provided, b) the chitosan porous layers of a) are neutralized and washed, c) a face ( 1 a, 2 a ) of each chitosan porous layer is acidified with an acid, d) a fiber reinforcement member ( 3 ) is provided and positioned between the two acidified faces of the two chitosan porous layers, e) the two chitosan porous layers and the fiber reinforcement member are let to dry to obtain a matrix having the fiber reinforcement member embedded therein.

The invention also relates to an implant comprising a matrix obtained according to such a method.

First claim

1 - 15 . (canceled)

16 . A method for preparing a chitosan-based matrix comprising a fiber reinforcement member embedded in the matrix comprising:
a) providing one or more solutions including chitosan, b) obtaining two separate chitosan porous layers from lyophilisation of the one or more solutions of chitosan, c) neutralizing the chitosan porous layers of b), d) washing the chitosan porous layers of c), e) applying an acid to a face of each of the chitosan porous layers of d), f) positioning a fiber reinforcement member between the two acidified faces of the two chitosan porous layers of e), g) drying the two chitosan porous layers and the fiber reinforcement member of f) to obtain a matrix having the fiber reinforcement member embedded therein.

17 . The method according to claim 16 , wherein providing one or more solutions including chitosan comprises a solution of chitosan in water, the chitosan including a concentration in the solution ranging from 0.1% to 10% by weight, relative to a total weight of the solution.

18 . The method according to claim 16 , wherein providing one or more solutions including chitosan comprises a solution of chitosan in water, the chitosan including a concentration in the solution ranging from 0.5% to 3% by weight, relative to a total weight of the solution.

19 . The method according to claim 16 , wherein providing one or more solutions including chitosan comprises a solution of chitosan in water, the chitosan including a concentration in the solution ranging from 0.8% to 1% by weight, relative to a total weight of the solution.

20 . The method according to claim 16 , further comprising adjusting a pH of the one or more solutions in a) to a value ranging from 3 to 5.

21 . The method according to claim 16 , further comprising adjusting a pH of the one or more solutions in a) to a value of 3.5.

22 . The method according to claim 20 , wherein adjusting a pH of the one or more solutions in a) comprises adding acetic acid to the one or more of solutions.

23 . The method according to claim 16 , wherein applying an acid according to e) comprises spraying a solution of the acid on the face.

24 . The method according to claim 16 , wherein applying an acid according to e) comprises applying an acid selected from acetic acid, hydrochloric acid, citric acid, sulphuric acid, lactic acid, and mixtures thereof.

25 . The method according to claim 16 , wherein applying an acid according to e) comprises applying acetic acid.

26 . The method according to claim 25 , wherein applying an acid according to e) comprises spraying a solution of acetic acid at 0.1 M on the face of each of the chitosan porous layers.

27 . The method according to claim 26 , wherein the spraying is repeated twice.

28 . The method according to claim 16 , further comprising impregnating the fiber reinforcement member with a solution of an acid before being positioned between the two acidified faces of the two chitosan porous layers of e).

29 . The method according to claim 28 , wherein the solution of an acid comprises an acid selected from acetic acid, hydrochloric acid, citric acid, sulphuric acid, lactic acid, and mixtures thereof.

30 . The method according to claim 28 , wherein the solution of an acid comprises acetic acid.

31 . The method according to claim 30 , wherein the solution of an acid comprises a solution of acetic acid at 0.1 M.

32 . The method according to claim 16 , wherein the chitosan porous layers and the fiber reinforcement member have substantially bidimensional structures, and an area of the fiber reinforcement member is smaller than an area of each chitosan porous layer.

33 . The method according to claim 16 , wherein the fiber reinforcement member is a porous mesh.

34 . The method according to claims 16 , further comprising applying a pressure on the chitosan porous layers in a direction of the fiber reinforcement member during and/or after the positioning a fiber reinforcement member between the two acidified faces of the two chitosan porous layers of e).

35 . The method according to claims 16 , wherein neutralizing the chitosan porous layers of b) comprises immersing the chitosan porous layers of a) in a basic solution.

36 . The method according to claim 35 , wherein the basic solution comprises NaOH or NH 4 OH in water or an alcohol.

37 . The method according to claims 16 , wherein washing the chitosan porous layers of c) comprises dipping the chitosan porous layers of c) in one or more baths of sterile water until a washing water with a pH close to 7 is obtained.

38 . The method according to claim 16 , wherein applying an acid to a face of each of the chitosan porous layers of d) further comprises obtaining a superficial layer on the face of each of the chitosan porous layers so as to cause a slight resolubilization of the superficial layer of each face.

39 . The method according to claim 38 , wherein the superficial layer comprises a thickness up to 5% of a total thickness of each chitosan porous layer.

40 . A chitosan-based matrix which has been obtained according to the method of claim 16 .

41 . An implant for reinforcing the abdominal wall, comprising at least one chitosan-based matrix obtained by the method according to claims 16 .

Assignees

• Sofradim Production

Inventors

• Serrero Aurelie
• Desorme Mylene

Classifications

• C08L5/08

  Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof · CPC title

• C08B37/003

  Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof · CPC title

• C08J5/046 Primary

  with synthetic macromolecular fibrous material · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• C08J2305/08

  Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof · CPC title