Method for isolating a sample well of a test card for analysis, and resulting test card

Patent metadata

Field	Value
Publication number	US-2016236193-A1
Application number	US-201615140678-A
Country	US
Kind code	A1
Filing date	Apr 28, 2016
Priority date	Oct 22, 2010
Publication date	Aug 18, 2016
Grant date	—

Abstract

The invention relates to a method for isolating at least one fluid sample well laid out in a test card for analyses, said test card including a plate ( 3 ) having at least one first main face from which is laid out at least one channel communicating with at least said well, this first face being coated with a membrane ( 17 ) provided with an adhesive ( 18 ) and which will cover said channel with a covering area ( 17 1 ), the method for isolation including a phase for introducing a fluid sample into at least said well and a phase for isolating said well consisting of exerting a force on at least one portion of the covering area ( 17 1 ) of the adhesive membrane ( 17 ) for ensuring displacement of the adhesive into an obturation area for the sample well.

First claim

1 - 11 . (canceled)

12 . An obturation device for applying a method for isolating at least one sample well ( 2 ) laid out in a test card ( 1 ) for analysis, said test card including a support ( 3 ) having at least one first main face ( 4 , 5 ) from which is laid out at least one channel ( 9 a, 9 b, 9 c ) communicating with at least said well ( 2 ), this first face ( 4 , 5 ) being coated with a membrane ( 17 ) provided with an adhesive ( 18 ) and which will cover said channel ( 9 a, 9 c ), with a covering area ( 17 1 ), characterized in that the device includes a system ( 22 ) for exerting a force on at least one portion of the covering area ( 17 1 ) of the adhesive membrane ( 17 ) for displacing it until causing it to at least locally fit the shape of the wall ( 9 1 ) of the channel ( 9 a, 9 c ) so as to isolate said well ( 2 ) with respect to the channel by driving out the adhesive by applying the force on the adhesive membrane.

13 . The obturation device according to claim 12 , characterized in that the system ( 22 ) for exerting a force includes a supporting member ( 21 ) with a section mating the section of the channel.

14 . The obturation device according to claim 13 , characterized in that the supporting member ( 21 ) has a rounded supporting end.

15 . The obturation device according to claim 12 , characterized in that the supporting member ( 21 ) is driven for moving closer/moving away relatively to the test card ( 1 ) in order to allow application of at least one portion of the covering area ( 17 1 ) of the adhesive membrane ( 17 ) on the wall ( 9 1 ) of the channel.

16 . The obturation device according to claim 13 , characterized in that the supporting member ( 21 ) is mounted so as to be mobile in rotation in order to roll on at least one portion of the length of the channel ( 9 a, 9 c ).

17 . A machine for processing test cards, characterized in that it includes an obturation device ( 22 ) according to claim 12 .

18 . The processing machine according to claim 17 , characterized in that the obturation device ( 22 ) is mounted for acting on the test card ( 1 ) during a translational movement of conveying means belonging to the processing machine.

Assignees

• Biomerieux Sa

Inventors

• Colin Bruno
• Paris Cecile

Classifications

• C12Q1/24

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• B01L2400/0638

  membrane valves, flap valves · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B01L2200/0689

  Sealing · CPC title