Chromatography column assembly

Patent metadata

Field	Value
Publication number	US-2016228792-A1
Application number	US-201415022813-A
Country	US
Kind code	A1
Filing date	Aug 15, 2014
Priority date	Sep 27, 2013
Publication date	Aug 11, 2016
Grant date	—

Abstract

The invention relates to a chromatography column assembly that offers an affordable self-packed column for performing chromatographic separation. The chromatography column assembly comprises a column tube having a first and a second opening at substantially opposite ends, and an end cap of either a snap fit design or a bolted design. The column assembly may further include an adaptor shaft inserted into the column through the end cap for achieving a desired bed volume. The column assembly may also include a second end cap enclosing the second opening, and an optional adaptor shaft may be inserted into the column through the second end cap, enabling further adjustment of the bed volume.

First claim

1 . A liquid chromatography column assembly comprising:
a column tube having a first opening and a second opening at substantially opposite ends; and a snap fit end cap that encloses the first opening.

2 . The liquid chromatography column assembly of claim 1 , wherein the assembly may take a backpressure of up to 2 bars.

3 . The liquid chromatography column assembly of claim 1 , further comprising:
an adaptor shaft inserted into the column from the first opening.

4 . The liquid chromatography column assembly of claim 3 , wherein the snap fit end cap contains internal thread and the adaptor shaft contains complementary thread.

5 . The liquid chromatography column assembly of claim 4 , further comprising a concealed pin across a part of the rim of the column tube near the first opening such that rotation of the adaptor shaft is prevented along with the end cap.

6 . The liquid chromatography column assembly of claim 4 , further comprising an O-ring to introduce friction between the adapter shaft and the column tube to prevent rotation of the adaptor shaft.

7 . The liquid chromatography column assembly of claim 1 , further comprising a second adaptor shaft inserted into the column from the second opening.

8 . The liquid chromatography column assembly of claim 1 , further comprising a second snap fit end cap that encloses the second opening.

9 . A liquid chromatography column assembly comprising:
a column tube having a first opening and a second opening at substantially opposite ends, each with a rim thereof; an end cap, bolted onto the rim of the column with a bolt thus enclosing the first opening; an end cap nut with internal threads with a retainer ring; and an adapter shaft with complementary threads and engaged with the end cap nut.

10 . The liquid chromatography column assembly of claim 9 , wherein the assembly may take a backpressure of up to 5 bars.

11 . The liquid chromatography column assembly of claim 9 , further comprising a pin stopper on the end cap such that rotation of the adaptor shaft is prevented while the end cap nut is been rotated.

12 . The liquid chromatography column assembly of any of claim 9 , further comprising a second adaptor shaft inserted into the column from the second opening.

13 . The liquid chromatography column assembly of any of claim 9 , further comprising a second end cap, bolted onto the rim of the column with a bolt thus enclosing the second opening.

14 . The liquid chromatography column assembly of any of claim 9 , wherein the adaptor shaft, at the end that is inserted into the column, includes a distribution plate to allow homogenous distribution of in-coming liquid in the column.

15 . A method for the separation of a biomolecule of interest from a mixture, which method comprising
(a) packing a chromatography column assembly of any of claim 1 with a suitable chromatography matrix; (b) passing the mixture over the chromatography column to adsorb the biomolecule of interest; (c) contacting the matrix with an elution buffer that releases the adsorbed biomolecule of interest; and (d) collecting the biomolecule of interest.

16 . A kit for making a chromatography column assembly, comprising, in separate compartments, a snap fit end cap and a column tube.

17 . The kit according to claim 16 , wherein the snap fit end cap includes internal thread, and the kit further comprises an adaptor shaft that includes complementary thread.

18 . The kit according to claim 17 , wherein the column tube contains a pin stopper on its rim.

19 . A kit for making a chromatography column assembly; comprising, in separate compartments, an end cap, a column tube, a bolt, an end cap nut with internal thread, a retainer ring, an adapter shaft complementary thread, wherein both the end cap and the column tube contains an opening for the bolt.

20 . The kit according to claim 19 , wherein the end cap includes a pin stopper that prevents rotation of the adaptor shaft while the end cap nut is been rotated in relation to the column tube.

21 . A kit according to any one of claim 19 , further comprises written or electronically saved instructions.

Assignees

• Ge Healthcare Bio Sciences Ab
• Ge Healthcare Bio-Science Ab

Inventors

• Rao Veena
• Ramakrishna Manoj
• Kamble Nikhil

Classifications

• G01N30/6021

  Adjustable pistons · CPC title

• B01D15/424 Primary

  Elution mode · CPC title

• B01D15/22

  relating to the construction of the column · CPC title

• G01N30/6004

  end pieces · CPC title

• C07K1/16

  by chromatography · CPC title