Device for capturing circulating cells

Patent metadata

Field	Value
Publication number	US-2016209418-A1
Application number	US-201514834024-A
Country	US
Kind code	A1
Filing date	Aug 24, 2015
Priority date	Mar 18, 2009
Publication date	Jul 21, 2016
Grant date	—

Abstract

The present invention provides devices and methods for capturing rare cells. The devices and methods described herein can be used to facilitate the diagnosis and monitoring of metastatic cancers.

First claim

1 - 47 . (canceled)

48 . A method of selectively isolating biological cells from a cell sample comprising:
contacting the cell sample to a device for capturing biological cells under conditions that permit binding agents of the device to selectively bind a subpopulation of biological cells, resulting in bound biological cells; and removing unbound biological cells from the device, wherein the device comprises:
a substrate comprising a nanostructured surface region; and
a plurality of binding agents attached to the nanostructured surface region of the substrate,
wherein the nanostructured surface region comprises a plurality of nanostructures each having a longitudinal dimension and a lateral dimension, the longitudinal dimension being at least ten times greater than the lateral dimension, and
wherein biological cells are selectively captured by the binding agents and the plurality of nanostructures acting in cooperation.

49 . A method according to claim 48 , further comprising washing the captured biological cells with an aqueous medium.

50 . A method according to claim 48 , wherein the cell sample comprises one or more of body fluid, plasma, saliva, spinal fluid, and urine.

51 . A method according to claim 48 , wherein the subpopulation of biological cells comprises circulating tumor cells.

52 . A method of selectively isolating at least one target biological cell from a cell sample comprising:
providing a cell sample comprising at least one target biological cell; and contacting the cell sample to a plurality of nanostructures to result in the target biological cell(s) being captured, wherein a plurality of binding agents specific for and which bind to the target biological cell(s) are attached to the nanostructures; under conditions effective for the target biological cell(s) to bind to the binding agents attached to the nanostructures, resulting in bound biological cell(s).

53 . A method according to claim 52 , further comprising washing the bound biological cell(s) with an aqueous medium.

54 . A method according to claim 52 , further comprising detecting the presence of the target biological cell(s).

55 . A method according to claim 52 , wherein the cell sample comprises one or more of body fluid, plasma, saliva, spinal fluid, and urine.

56 . A method according to claim 52 , wherein the target biological cell(s) comprises a circulating tumor cell(s).

57 . A method according to claim 52 , wherein the plurality of binding agents comprises one or more of DNA, peptides, aptamers, and antibodies.

58 . A method according to claim 52 , wherein said plurality of binding agents comprises a plurality of over-expressed antibodies for tumor cells.

59 . A method according to claim 52 , wherein said plurality of binding agents is a plurality of anti-epithelial-cell adhesion molecule antibodies (anti-EpCAM).

60 . A method according to claim 52 , wherein said plurality of binding agents is a plurality of antibodies for immune cells.

61 - 63 . (canceled)

Assignees

• Tseng Hsian-Rong
• Wang Shutao
• Wang Hao
• Liu Kan
• Univ California

Inventors

• Tseng Hsian-Rong
• Wang Shutao
• Wang Hao
• Liu Kan

Classifications

• G01N33/5759 Primary

  involving compounds localised on the membrane of tumour or cancer cells · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/0877

  Flow chambers · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title

• B01L2300/0887

  Laminated structure · CPC title