Systems and methods for production of mixed fatty esters

Patent metadata

Field	Value
Publication number	US-2016208295-A1
Application number	US-201514599438-A
Country	US
Kind code	A1
Filing date	Jan 16, 2015
Priority date	Aug 18, 2008
Publication date	Jul 21, 2016
Grant date	—

Abstract

Disclosed herein are various embodiments regarding the production of fatty acid methyl esters. Disclosed herein are various embodiments regarding the use of methanol compositions for the production of fatty esters.

First claim

1 - 41 . (canceled)

42 . A genetically modified bacterial cell for production of a fatty ester, comprising an exogenous nucleic acid sequence encoding an alcohol forming polypeptide and an alcohol acetyltransferase polypeptide capable of catalyzing a conversion of a thioester and an alcohol, wherein said bacterial cell converts a renewable carbon source into a fatty ester.

43 . The bacterial cell of claim 42 , wherein said alcohol acetyltransferase polypeptide is alcohol acetyltransferase of EC 2.3.1.84.

44 . The bacterial cell of claim 43 , wherein said alcohol forming polypeptide is fatty alcohol forming acyl-CoA reductase of EC 1.1.1.*.

45 . The bacterial cell of claim 44 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding acyl-CoA synthase.

46 . The bacterial cell of claim 45 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding a thioesterase.

47 . The bacterial cell of claim 42 , wherein said alcohol forming polypeptide is acyl-CoA reductase of EC 1.1.1.50.

48 . The bacterial cell of claim 47 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding acyl-CoA synthase.

49 . The bacterial cell of claim 48 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding a thioesterase.

50 . The bacterial cell of claim 49 , wherein said cell is further genetically modified to express an exogenous nucleic acid sequence encoding an alcohol dehydrogenase of E.C. 1.1.1.1.

51 . The bacterial cell of claim 42 , wherein said bacterial cell is selected from the group consisting of Escherichia coli, Arthrobacter, Rhodotorula glutinins, Acinetobacter, Botryococcus braunii, Vibrio furnissii, Micrococcus leuteus, Stenotrophomonas maltophilia, Bacillus subtilis and cyanobacteria.

52 . The bacterial cell of claim 42 , wherein the fatty ester is released into a fermentation media.

Assignees

• Reg Life Sciences Llc

Inventors

• Lee Grace J
• Hu Zhihao

Classifications

• C07C69/587

  Monocarboxylic acid esters having at least two carbon-to-carbon double bonds · CPC title

• C10L1/026

  for compression ignition · CPC title

• C12P7/649 Primary

  Biodiesel, i.e. fatty acid alkyl esters · CPC title

• C10L2200/0476

  Biodiesel, i.e. defined lower alkyl esters of fatty acids first generation biodiesel · CPC title

• C07C69/533

  Monocarboxylic acid esters having only one carbon-to-carbon double bond · CPC title