Yeast cell having decreased rgt1 activity, method of producing the same, and method of producing product using the same

Patent metadata

Field	Value
Publication number	US-2016208270-A1
Application number	US-201514980983-A
Country	US
Kind code	A1
Filing date	Dec 28, 2015
Priority date	Dec 26, 2014
Publication date	Jul 21, 2016
Grant date	—

Abstract

A recombinant yeast cell having a decreased RGT1 protein activity and an increased ability to produce a glycolytic intermediate or a glycolytic intermediate-derived substance, compared to a parent cell; methods of producing the same; and methods of producing the glycolytic intermediate or the glycolytic intermediate-derived substance using the same.

First claim

What is claimed is:

1 . A genetically engineered yeast cell comprising a genetic modification that decreases RGT1 protein activity compared to the RGT1 protein activity of a parent cell thereof, wherein the genetically engineered yeast cell has increased productivity of a glycolytic intermediate compared to a parent cell thereof.

2 . The genetically engineered yeast cell of claim 1 , wherein the genetic modification is a disruption mutation of a gene encoding RGT1.

3 . The genetically engineered yeast cell of claim 1 , wherein the genetically engineered yeast cell further comprises a genetic modification that decreases HXK2 protein activity compared to the HXK2 protein activity of a parent cell thereof.

4 . The genetically engineered yeast cell of claim 3 , wherein the genetic modification that decreases the HXK2 protein activity is a disruption mutation of a gene encoding HXK2.

5 . The genetically engineered yeast cell of claim 1 , wherein the yeast cell consumes glucose at an increased glucose consumption rate compared to the parent cell.

6 . The genetically engineered yeast cell of claim 1 , wherein the glycolytic intermediate is dihydroxyacetone phosphate (DHAP), glyceraldehyde-3-phosphate (GAP), pyruvate, or a combination thereof.

7 . The genetically engineered yeast cell of claim 1 , wherein the genetically engineered yeast cell has increased productivity of glycerol-3-phosphate (G3P), glycerol, acetyl-CoA, ethanol, acetic acid, lactate, a TCA cycle intermediate, or a combination thereof.

8 . The genetically engineered yeast cell of claim 8 , wherein the genetically engineered yeast cell has increased productivity of a TCA cycle intermediate comprising citric acid, itaconic acid, isocitrate, oxalosuccinate, alpha-ketoglutarate, succinic acid, succinyl-CoA, fumaric acid, malate, oxaloacetate, or a combination thereof, and the pyruvate-derived substance is a product derived from the TCA cycle intermediate an the product is 1,3-butanediol (1,3-BDO), 1,4-butanediol (1,4-BDO), butanol, isobutanol, or a combination thereof.

9 . The genetically engineered yeast cell of claim 1 , wherein the genetically engineered yeast cell further comprises an exogenous gene that encodes an enzyme that catalyzes conversion of pyruvate to lactate.

10 . The genetically engineered yeast cell of claim 1 , wherein the RGT1 protein has an amino acid sequence of SEQ ID NO. 1.

11 . The genetically engineered yeast cell of claim 3 , wherein the HXK2 protein has an amino acid sequence of SEQ ID NO. 3.

12 . The genetically engineered yeast cell of claim 1 , wherein the genetically engineered yeast cell is a strain belonging to Saccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Shizosaccharomyces, Issachenkia , or Hansenula.

13 . The genetically engineered yeast cell of claim 1 , wherein the genetically engineered yeast cell further comprises a genetic modification that decreases the activity of an enzyme that catalyzes conversion of acetaldehyde to ethanol, an enzyme that catalyzes conversion of pyruvate to acetaldehyde, an enzyme that catalyzes conversion of lactate to pyruvate, an enzyme that catalyzes conversion of dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate (G3P), an enzyme that catalyzes conversion of glycerol-3-phosphate (G3P) to glycerol, an enzyme that catalyzes conversion of acetaldehyde to acetate, or a combination thereof.

14 . The genetically engineered yeast cell of claim 13 , wherein the enzyme that catalyzes conversion of acetaldehyde to ethanol belongs to EC 1.1.1.1, the enzyme that catalyzes conversion of pyruvate to acetaldehyde belongs to EC 4.1.1.1, the enzyme that catalyzes conversion of lactate to pyruvate belongs to EC 1.1.2.4 or EC 1.1.2.3, the enzyme that catalyzes conversion of DHAP to G3P belongs to EC 1.1.1.8, the enzyme that catalyzes conversion of glycerol-3-phosphate (G3P) to glycerol belongs to EC 3.1.3.21, and the enzyme that catalyzes conversion of acetaldehyde to acetate belongs to EC 1.2.1.4.

15 . The genetically engineered yeast cell of claim 13 , wherein the enzyme that catalyzes conversion of acetaldehyde to ethanol is alcohol dehydrogenase (ADH), the enzyme that catalyzes conversion of pyruvate to acetaldehyde is pyruvate decarboxylase (PDC), the enzyme that catalyzes conversion of lactate to pyruvate is lactate cytochrome-c oxidoreductase (CYB2), the enzyme that catalyzes conversion of DHAP to G3P is NAD-dependent glycerol-3-phosphate dehydrogenase (GPD), the enzyme that catalyzes conversion of glycerol-3-phosphate to glycerol is glycerol phosphate phosphatase (GPP), and the enzyme that catalyzes conversion of acetaldehyde to acetate is acetaldehyde dehydrogenase.

16 . The genetically engineered yeast cell of claim 13 , wherein a gene encoding the enzyme that catalyzes conversion of acetaldehyde to ethanol, a gene encoding the enzyme that catalyzes conversion of pyruvate to acetaldehyde, a gene encoding the enzyme that catalyzes conversion of lactate to pyruvate, a gene encoding the enzyme that catalyzes conversion of dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate (G3P), a gene encoding the enzyme that catalyzes conversion of glycerol-3-phosphate to glycerol, a gene encoding the enzyme that catalyzes conversion of acetaldehyde to acetate, or a combination thereof comprises a disruption mutation.

17 . A method of producing a glycolytic intermediate or glycolytic intermediate-derived substance, the method comprising:
culturing the genetically engineered yeast cell of claim 1 in a cell culture medium to obtain a culture, whereby the genetically engineered yeast cell produces a glycolytic intermediate or glycolytic intermediate-derived substance in the culture; and isolating the glycolytic intermediate from the culture.

18 . The method of claim 17 , wherein the culturing is performed under microaerobic conditions.

19 . A method of producing a genetically engineered yeast cell having an ability to produce lactate, the method comprising:
introducing a gene encoding an enzyme that catalyzes the conversion of pyruvate into lactate into a yeast cell; and disrupting a gene encoding an RGT1 protein in the yeast cell.

20 . The method of claim 19 , further comprising disrupting a gene encoding an HXK2 protein in the yeast cell.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Lee Jinsuk
• Kang Changduk
• Lee Seunghyun
• Cho Kwangmyung

Classifications

• C12P7/00 Primary

  Preparation of oxygen-containing organic compounds · CPC title

• C12N15/81 Primary

  for yeasts · CPC title

• C12P7/62 Primary

  Carboxylic acid esters · CPC title

• C12Y301/03021

  Glycerol-1-phosphatase (3.1.3.21) · CPC title

• C07K14/395

  from Saccharomyces · CPC title