Methods for selecting competent oocytes and competent embryos with high potential for pregnancy outcome

Patent metadata

Field	Value
Publication number	US-2016201128-A1
Application number	US-201514808204-A
Country	US
Kind code	A1
Filing date	Jul 24, 2015
Priority date	Aug 6, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

The present invention relates to a method for selecting a competent oocyte or a competent embryo.

First claim

1 . A method for selecting an oocyte that will produce, upon fertilization, a viable embryo with a high implantation rate leading to pregnancy, comprising a step of measuring in a cumulus cell surrounding said oocyte the expression level of at least 5 microRNA selected from the group consisting of hsa-mir-103-1, hsa-mir-103-2, hsa-mir-1826, hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7f, hsa-mir-1244, hsa-mir-182, hsa-mir-21, hsa-mir-30a, hsa-mir-30d, hsa-mir-320a, hsa-mir-508, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-1974, hsa-mir-146b, hsa-mir-886, hsa-mir-210, hsa-mir-1979, hsa-mir-125a, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21.

2 - 10 . (canceled)

11 . The method according to claim 1 wherein said at least 5 microRNA are selected from the group consisting of hsa-mir-508, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-103-1, hsa-mir-103-2, hsa-mir-1974, hsa-mir-1826, SEQ ID NO: 7, SEQ ID NO: 8 and SEQ ID NO: 12.

12 . The method according to claim 1 wherein said at least 5 microRNA are selected from the group consisting of hsa-mir-508, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-103-1, has-mir-103-2, hsa-mir-1974, hsa-mir-1826, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 12, hsa-let-7b, hsa-let-7c, hsa-mir-182, hsa-mir-21, hsa-mir-30a and hsa-mir-30d.

13 . The method according to claim 1 wherein said at least 5 microRNA are selected from the group consisting of hsa-mir-182, hsa-mir-320a, hsa-mir-210, hsa-mir-21 and has-let-7a-1.

14 . The method according to claim 1 comprising a further step of measuring in a cumulus cell surrounding said oocyte the expression level of one or more genes selected from the group consisting of ATF3, SIAT6, PRKACA, PLA2G5, GPC6, GOS2, RBMS1, NFIC, SLC40A1 and WNT6.

15 . A method for selecting an embryo with a high implantation rate leading to pregnancy, comprising a step of measuring in a cumulus cell surrounding said embryo the expression level of at least 5 microRNA selected from the group consisting of hsa-mir-103-1, hsa-mir-103-2, hsa-mir-1826, hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7f, hsa-mir-1244, hsa-mir-182, hsa-mir-21, hsa-mir-30a, hsa-mir-30d, hsa-mir-320a, hsa-mir-508, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-1974, hsa-mir-146b, hsa-mir-886, hsa-mir-210, hsa-mir-1979, hsa-mir-125a, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21.

16 . The method according to claim 15 wherein said at least 5 microRNA are selected from the group consisting of hsa-mir-508, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-103-1, has-mir-103-2, hsa-mir-1974, hsa-mir-1826, SEQ ID NO: 7, SEQ ID NO: 8 and SEQ ID NO: 12.

17 . The method according to claim 15 wherein said at least 5 microRNA are selected from the group consisting of hsa-mir-508, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-103-1, has-mir-103-2, hsa-mir-1974, hsa-mir-1826, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 12, hsa-let-7b, hsa-let-7c, hsa-mir-182, hsa-mir-21, hsa-mir-30a and hsa-mir-30d.

18 . The method according to claim 15 wherein said at least 5 microRNA are selected from the group consisting of hsa-mir-182, hsa-mir-320a, hsa-mir-210, hsa-mir-21 and has-let-7a-1.

19 . The method according to claim 15 comprising a further step of measuring in a cumulus cell surrounding said embryo the expression level of one or more genes selected from the group consisting of ATF3, SIAT6, PRKACA, PLA2G5, GPC6, GOS2, RBMS1, NFIC, SLC40A1 and WNT6.

20 . A method for selecting an oocyte that will produce, upon fertilization, a viable embryo with a high implantation rate leading to pregnancy or an embryo with a high implantation rate leading to pregnancy, comprising a step of measuring in the culture medium of cumulus cell having surrounded said oocyte or said embryo the expression level of at least 5 microRNA selected from the group consisting of hsa-mir-103-1, hsa-mir-103-2, hsa-mir-1826, hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7f, hsa-mir-1244, hsa-mir-182, hsa-mir-21, hsa-mir-30a, hsa-mir-30d, hsa-mir-320a, hsa-mir-508, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-1974, hsa-mir-146b, hsa-mir-886, hsa-mir-210, hsa-mir-1979, hsa-mir-125a, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20 and SEQ ID NO: 21.

21 . A method for selecting an oocyte that will produce, upon fertilization, a viable embryo with a high implantation rate leading to pregnancy or an embryo with a high implantation rate leading to pregnancy, comprising a step of measuring in a bodily fluid the expression level of at least 5 microRNA selected from the group consisting of hsa-mir-103-1, hsa-mir-103-2, hsa-mir-1826, hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7f, hsa-mir-1244, hsa-mir-182, hsa-mir-21, hsa-mir-30a, hsa-mir-30d, hsa-mir-320a, hsa-mir-508, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-16-1, hsa-mir-16-2, hsa-mir-1974, hsa-mir-146b, hsa-mir-886, hsa-mir-210, hsa-mir-1979, hsa-mir-125a, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO:24 and SEQ ID NO: 25.

22 . An isolated nucleic acid molecule having the nucleotide sequence as set forth in SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO:24 and SEQ ID NO: 25.

23 . A kit comprising means for measuring the expression level of the microRNA of Table I.

Assignees

• Hamamah Samir
• Assou Said
• Inst Nat Sante Rech Med
• Univ Montpellier 1
• Ct Hospitalier Universitaire Montpellier

Inventors

• Hamamah Samir
• Assou Said

Classifications

• C12N2320/10

  in screening processes · CPC title

• C12N2310/141

  MicroRNAs, miRNAs · CPC title

• C12N15/111

  General methods applicable to biologically active non-coding nucleic acids · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12Q1/6881 Primary

  for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes · CPC title