Particle manipulation method and particle manipulation device

Patent metadata

Field	Value
Publication number	US-2016201114-A1
Application number	US-201314901791-A
Country	US
Kind code	A1
Filing date	Jul 3, 2013
Priority date	Jul 3, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

Disclosed is a particle manipulation method including steps of moving particles, which exist in a water-based liquid, into a gelled medium that is insoluble or hardly soluble in the water-based liquid in the water-based liquid, and moving the particles, which exist in the gelled medium, to the outside of the gelled medium. Preferably, the gelled medium is a gel that contains chemically crosslinking polymer. Preferably, the gelled medium has a consistency of 340 to 475. In one embodiment, the movement of the particles existing in the water-based liquid to the gelled medium and the movement of the particles existing inside the gelled medium to the water-based liquid are carried out inside a device, the device being loaded with a plurality of water-based liquids and a gelled medium interposed among the water-based liquids.

First claim

1 . A particle manipulation method comprising the steps of:
moving particles, which exist in a water-based liquid, into a gelled medium that is insoluble or hardly soluble in the water-based liquid; and moving the particles, which exist in the gelled medium, to the outside of the gelled medium, wherein the particles are capable of selectively immobilizing a specific substance selected from a group consisting of nucleic acids, proteins, sugars, lipids, antibodies, receptors, antigens, ligands and cells, and the gelled medium is a silicone gel containing chemically crosslinked organopolysiloxane.

2 . The particle manipulation method according to claim 1 wherein
the gelled medium has a consistency of 340 to 475.

3 - 4 . (canceled)

5 . The particle manipulation method according to claim 1 , wherein the gelled medium further contains an oil solution.

6 . The particle manipulation method according to claim 1 , wherein in movement of the particles into the gelled medium from the water-based liquid and in movement of the particles in the gelled medium, the water-based liquid associated with the particles is separated from the particles.

7 . The particle manipulation method according to claim 1 , wherein the particles are moved into a different water-based liquid in the step of moving the particles to the outside of the gelled medium.

8 . The particle manipulation method according to claim 7 , wherein
in a device loaded with a plurality of water-based liquids with the gelled medium interposed among the water-based liquids, the step of moving particles, which exist in a water-based liquid, into a gelled medium, and the step of moving the particles, which exist in the gelled medium, into a different water-based liquid are carried out two or more times.

9 . The particle manipulation method according to claim 8 , wherein at least two of the plurality of water-based liquids have mutually different compositions.

10 . The particle manipulation method according to claim 1 , wherein the particles are magnetic particles, and moving the particles are performed by magnetic field manipulation.

11 - 12 . (canceled)

13 . The particle manipulation method according to claim 1 , wherein the nucleic acid immobilized on the particles is eluted in at least one water-based liquid.

14 . The particle manipulation method according to claim 1 , wherein an antigen-antibody reaction is performed in at least one water-based liquid.

15 . A particle manipulation device comprising: a water-based liquid; a gelled medium that is insoluble or hardly soluble in the water-based liquid; a container for holding the water-based liquid and the gelled medium; and particles to be moved in the container, wherein
the particles are capable of selectively immobilizing a specific substance selected from a group consisting of nucleic acids, proteins, sugars, lipids, antibodies, receptors, antigens, ligands and cells, and the gelled medium is a silicone gel containing chemically crosslinked organopolysiloxane.

16 . The particle manipulation device according to claim 15 , wherein
the gelled medium has a consistency of 340 to 475.

17 . A particle manipulation device preparation kit for preparing the particle manipulation device of claim 15 , comprising: a water-based liquid; a gelled medium that is insoluble or hardly soluble in the water-based liquid; and particles, wherein
the particles are capable of selectively immobilizing a specific substance selected from a group consisting of nucleic acids, proteins, sugars, lipids, antibodies, receptors, antigens, ligands and cells, and the gelled medium is a silicone gel containing chemically crosslinked organopolysiloxane.

18 . The particle manipulation device preparation kit according to claim 17 , wherein the gelled medium has a consistency of 340 to 475.

Assignees

• Shimadzu Corp
• Kazusa Dna Res Inst

Inventors

• Ohashi Tetsuo
• Ohara Osamu
• Nonaka Ken

Classifications

• B03C1/0332 Primary

  using permanent magnets · CPC title

• C12N15/1013

  by using magnetic beads · CPC title

• B03C1/01

  by addition of magnetic adjuvants · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B01D15/1892

  the sorbent material moving as a whole, e.g. continuous annular chromatography, true moving beds or centrifugal chromatography · CPC title