Process for the Bioconversion of C3-C13 Alkanes to C3-C13 Primary Alcohols

Patent metadata

Field	Value
Publication number	US-2016201093-A1
Application number	US-201414908293-A
Country	US
Kind code	A1
Filing date	Jul 21, 2014
Priority date	Jul 31, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

A process for preparing linear or branched primary alcohols with 3 to 13 carbon atoms from linear or branched alkanes with 3 to 13 carbon atoms by incubating a host organism having a functional P153 enzyme under elevated pressure in the presence of oxygen.

First claim

1 . A process for preparing a linear or branched primary alcohol with 3 to 13 carbon atoms from a linear or branched alkane with 3 to 13 carbon atoms by incubating a host organism having a functional P153 enzyme under elevated pressure in the presence of oxygen.

2 . The process according to claim 1 , wherein the host organism is unable to use linear or branched primary alcohols with 3 to 13 carbon atoms as a carbon source.

3 . The process according to claim 2 , wherein the host organism is E. coli.

4 . The process according to claim 1 , wherein the pressure is from 2-20 bar.

5 . The process according to claim 1 , wherein the incubation temperature is from 0-50° C.

6 . The process according to claim 1 , wherein the P153 enzyme is isolated from the organism selected from the group of Pseudomonas, Polaromonas and Mycobacterium.

7 . The process according to claim 6 , wherein the P153 enzyme has a polypeptide sequence selected from the group which is formed by SEQ ID NO: 1, SEQ ID NO:2 and derivatives of SEQ ID NOS: 1 and 2 wherein the derivatives have up to three amino acid exchanges compared to SEQ ID NOS: 1 and 2.

8 . The process according to claim 1 , wherein a minor amount of a linear or branched secondary alcohol with 3 to 13 carbon atoms is produced in addition to the linear or branched primary alcohol with 3 to 13 carbon atoms.

9 . The process according to claim 1 , wherein the alkane is n-butane, n-octane, 2-ethylhexane, n-nonane, n-decane, a mixture of n-nonane, methyloctane, a dimethylheptane, and ethylheptane, or a mixture of propylhexane, isopropylhexane, and methylpropylpentane, and the linear or branched primary alcohol with 3 to 13 carbon atoms is 1-butanol, 1-octanol, 2-ethyl-1-hexanol, 1-nonanol, 1-decanol, a mixture of 1-nonanol, methyl-1-octanol, dimethyl-1-heptanol, and ethyl-1-heptanol, or a mixture of propyl-1-hexanol, isopropyl-1-hexanol, and methylpropyl-1-pentanol.

10 . The process according to claim 1 , wherein the alkane is n-butane, n-octane, 2-ethylhexane, n-nonane or n-decane, and the linear or branched primary alcohol is 1-butanol, 1-octanol, 2-ethyl-1-hexanol, 1-nonanol or 1-decanol.

Assignees

• Basf Se

Inventors

• Breuer Michael
• Breitscheidel Boris
• Wagner Hans-Günter
• Kratz Detlef
• Hauer Bernhard
• Scheps Daniel
• Nebel Bernd

Classifications

• C12P7/16 Primary

  Butanols · CPC title

• C12P7/04

  acyclic · CPC title

• C12Y118/01001

  Rubredoxin--NAD+ reductase (1.18.1.1) · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12Y114/15003

  Alkane 1-monooxygenase (1.14.15.3) · CPC title