Plant resistant to helminthosporium turcicum

Patent metadata

Field	Value
Publication number	US-2016201080-A1
Application number	US-201414916671-A
Country	US
Kind code	A1
Filing date	Sep 3, 2014
Priority date	Sep 4, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

The present invention provides an improved Helminthosporium turcicum -resistant plant, in particular a maize plant which comprises a polynucleotide with one or more resistance-conferring genes, for example on a truncated chromosome fragment from the accession Pepitilla, as well as a cell, a tissue, a part, grain and seeds thereof, an isolated polynucleotide which comprises one or more resistance-conferring genes against Helminthosporium turcicum , a vector, a transgenic plant cell and a transgenic plant containing this polynucleotide. Furthermore, the invention encompasses suitable markers and their use in introducing resistance or the transgene into a plant, as well as the identification of improved maize plants which comprise a truncated chromosome fragment.

First claim

1 . A maize plant into the genome of which a chromosome fragment from a donor Pepitilla has been integrated,
wherein the chromosome fragment comprises an interval of the donor which exhibits donor alleles in accordance with a haplotype of Table 2 and comprises a polynucleotide which confers resistance to Helminthosporium turcicum in the maize plant; and
wherein the chromosome fragment:
a) does not contain an interval of the donor between a marker in a first marker region which is flanked by the markers SYN14136 and PZE-108076510 and a marker in a second marker region which is flanked by the markers SYN24931 and PZE-108077560; and/or b) does not contain an interval of the donor between a marker in a third marker region which is flanked by the markers PZE-108093423 and PZE-108093748 and a marker in a fourth marker region which is flanked by the markers MA0004 and MA0005; and/or c) does not contain an interval of the donor between a marker in a fifth marker region which is flanked by the markers MA0006 and PZE-108097482 and a marker in a sixth marker region which is flanked by the markers PZE-108107671 and SYN4196.

2 . The maize plant as claimed in claim 1 , wherein the chromosome fragment furthermore does not contain an interval of the donor between a marker in the second marker region and a marker in the third marker region.

3 . The maize plant as claimed in claim 1 , wherein a flowering time for the maize plant and/or a silage yield of the maize plant corresponds to that of a comparative maize plant into the genome of which the chromosome fragment from the donor Pepitilla has not been integrated.

4 . The maize plant as claimed in claim 1 , wherein the polynucleotide which confers resistance to Helminthosporium turcicum comprises:
(a) a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, and 15; (b) a nucleotide sequence which is at least 80% identical to the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, and 15; (c) a nucleic acid molecule hybridizing with the complementary strand of a nucleic acid molecule in accordance with (a) or (b) under stringent conditions; (d) a nucleotide sequence coding for a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, and 16; (e) a nucleotide sequence coding for a polypeptide with an amino acid sequence which has at least 80% identity with an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, and 16; or (f) a nucleotide sequence comprising a part of the nucleotide sequence in accordance with any one of (a) to (e).

5 . Cells, tissues or parts of the maize plant as claimed in claim 1 .

6 . Grain or seeds from the maize plant as claimed in claim 1 .

7 . A method for identifying a Helminthosporium turcicum -resistant maize plant, the genome of which maize plant has a chromosome fragment from a donor Pepitilla integrated therein, comprising:
detecting at least two alleles in the genome of the plant; (i) wherein at least one allele is localized in a genomic segment which is flanked by a marker in a first marker region which is flanked by the markers SYN14136 and PZE-108076510, a second marker region which is flanked by the markers SYN24931 and PZE-108077560, a third marker region which is flanked by the markers PZE-108093423 and PZE-108093748, or a fourth marker region which is flanked by the markers MA0004 and MA0005 and by a polynucleotide which confers resistance against Helminthosporium turcicum in the maize plant; and (ii) wherein at least one allele is localized in a genomic segment which is flanked by the polynucleotide which confers resistance against Helminthosporium turcicum and by a marker in a sixth marker region which is flanked by the markers PZE-108107671 and SYN4196 or a fifth marker region which is flanked by the markers MA0006 and PZE-108097482.

8 . A method for increasing the yield of a H. turcicum -resistant maize plant as claimed in claim 1 , the method comprising removing:
a) an interval of the donor Pepitilla between a marker in a first marker region which is flanked by the markers SYN14136 and PZE-108076510 and a marker in a second marker region which is flanked by the markers SYN24931 and PZE-108077560; and/or b) an interval of the donor Pepitilla between a marker in a third marker region which is flanked by the markers PZE-108093423 and PZE-108093748 and a marker in a fourth marker region which is flanked by the markers MA0004 and MA0005; and/or c) an interval of the donor Pepitilla between a marker in a fifth marker region which is flanked by the markers MA0006 and PZE-108097482 and a marker in a sixth marker region which is flanked by the markers PZE-108107671 and SYN4196.

9 . An isolated oligonucleotide which comprises a nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 41-49, 53-100, and 229-250, wherein the oligonucleotide corresponds to at least one of the first marker region which is flanked by the markers SYN14136 and PZE-108076510, the second marker region which is flanked by the markers SYN24931 and PZE-108077560, the third marker region which is flanked by the markers PZE-108093423 and PZE-108093748, the fourth marker region which is flanked by the markers MA0004 and MA0005, the fifth marker region, which is flanked by the markers MA0006 and PZE-108097482, and the sixth marker region which is flanked by the markers PZE-108107671 and SYN4196 as claimed in claim 8 .

10 . The method of claim 7 , wherein the identification of a Helminthosporium turcicum -resistant maize plant comprises the use of an oligonucleotide which comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 17-250.

11 . An isolated polynucleotide which comprises:
(a) a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, and 15; (b) a nucleotide sequence which is at least 80% identical to the nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, and 15; (c) a nucleic acid molecule which hybridizes with the complementary strand of a nucleic acid molecule in accordance with (a) or (b) under stringent conditions; (d) a nucleotide sequence which codes for a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, and 16; or (e) a nucleotide sequence which codes for a polypeptide with an amino acid sequence which has at least 80% identity with an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, and 16.

12 . A vector or a mobile genetic element comprising the polynucleotide of claim 11 .

13 . A plant cell comprising the polynucleotide of claim 11 , wherein said polynucleotide is heterologous to said plant cell.

14 . A plant comprising the polynucleotide of claim 11 , wherein said polynucleotide is heterologous to said plant.

15 . Grain or seeds from the plant of claim 14 .

16 . A plant cell comprising the vector or mobile genetic element of claim 12 .

17 . A plant comprising the vector or mobile genetic element of claim 12 .

18 . A plant comprising the plant cell of claim 13 .

19 . A plant comprising the plant cell of claim 16 .

20 . Grain or seeds comprising the polynucleotide of claim 11 , wherein said polynucleotide is heterologous to said grain or seeds.

Assignees

• Ouzunova Milenaö
• Scheuermann Daniela
• Keller Beat
• Krattinger Simon
• Wicker Thomas
• Herren Gerhard
• Hurni Severine
• Kessel Bettina
• Presterl Thomas
• Knaak Carsten
• Kws Saat Se & Co Kgaa
• Univ Zuerich

Inventors

• Ouzunova Milena
• Scheuermann Daniela
• Keller Beat
• Krattinger Simon
• Wicker Thomas
• Herren Gerhard
• Hurni Severine
• Kessel Bettina
• Presterl Thomas
• Knaak Carsten

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C07K14/415

  from plants · CPC title

• C12Q2600/158

  Expression markers · CPC title