Method for producing porous cellulose particles, and porous cellulose particles

Patent metadata

Field	Value
Publication number	US-2016200835-A1
Application number	US-201414913374-A
Country	US
Kind code	A1
Filing date	Aug 13, 2014
Priority date	Sep 2, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

One embodiment shows a method for producing porous cellulose particles, including:
(a) dissolving cellulose diacetate into a solvent to prepare a cellulose diacetate solution; (b) dispersing the cellulose diacetate solution into a medium immiscible with the cellulose diacetate solution to obtain a dispersed system; (c) cooling the dispersed system; (d) adding a poor solvent to the cooled dispersed system to precipitate cellulose diacetate particles; and (e) saponifying the cellulose diacetate particles.

First claim

1 . A method for producing porous cellulose particles, comprising:
(a) dissolving cellulose diacetate into a solvent to prepare a cellulose diacetate solution; (b) dispersing the cellulose diacetate solution into a medium immiscible with the cellulose diacetate solution to obtain a dispersed system; (c) cooling the dispersed system; (d) adding a poor solvent to the cooled dispersed system to precipitate cellulose diacetate particles; and (e) saponifying the cellulose diacetate particles.

2 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (a), the solvent for the cellulose diacetate solution is an acetic acid aqueous solution or cyclohexanone.

3 . The method for producing porous cellulose particles according to claim 2 , wherein, in the (a), the solvent for the cellulose diacetate solution is an acetic acid aqueous solution and a content of acetic acid in the acetic acid aqueous solution is 80 to 95% by weight based on the acetic acid aqueous solution.

4 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (d), the poor solvent is water, alcohols, glycols or a mixed liquid thereof.

5 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (b), the medium immiscible with the cellulose diacetate solution is water or an organic medium.

6 . The method for producing porous cellulose particles according to claim 5 , wherein, in the (b), the medium immiscible with the cellulose diacetate solution is an organic medium, and the organic medium is toluene or o-dichlorobenzene.

7 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (a), the cellulose diacetate has an acetylation degree of 45 to 57%.

8 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (a), a content of cellulose diacetate in the cellulose diacetate solution is 3 to 20% by weight based on the cellulose diacetate solution.

9 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (a), the cellulose diacetate is dissolved into the solvent at a temperature of 25° C. to 100° C.

10 . The method for producing porous cellulose particles according to claim 9 , wherein, in the (a), the cellulose diacetate is dissolved into the solvent at a temperature of 40° C. to 100° C.

11 . The method for producing porous cellulose particles according to claim 1 , wherein, in the (c), the cooling is performed by cooling the system to a temperature of 0° C. to 40° C.

12 . Porous cellulose particles, which may be produced by the method for producing porous cellulose particles according to claim 1 .

13 . The porous cellulose particles according to claim 12 , wherein a content of cellulose diacetate in the cellulose diacetate solution in the (a) is 6 to 12% by weight based on the cellulose diacetate solution, the cooling in the (c) is performed by cooling the system to a temperature of 0° C. to 30° C., a Kav value measured using PEG having a molecular weight of 8,000 is 0.01 or more and 0.52 or less, and a Kav value measured using PEG having a molecular weight of 12,000 is 0.001 or more and 0.45 or less.

14 . A media for chromatography, containing the porous cellulose particles according to claim 12 or the porous cellulose particles modified.

15 . The media for chromatography according to claim 14 , used for isolating or purifying virus particles.

16 . The media for chromatography according to claim 15 , used for isolating or purifying influenza virus particles or hepatitis B virus particles, wherein the media contains porous cellulose particles modified with a sulfate group-containing group or a sulfonate group-containing group, and an S content is 800 to 5,000 μg/g based on the porous cellulose particles modified.

Assignees

• Jnc Corp

Inventors

• Kurabayashi Toru
• Aoyama Shigeyuki
• Uchida Akihiro

Classifications

• C08J3/14

  by precipitation from solutions {(C08J3/122 takes precedence)} · CPC title

• B01J20/3085

  Chemical treatments not covered by groups B01J20/3007 - B01J20/3078 · CPC title

• B01J39/22

  Cellulose or wood; Derivatives thereof · CPC title

• B01J39/26

  Cation exchangers for chromatographic processes · CPC title

• B01J20/24

  Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives · CPC title